ELISA Kit DIY Materials for Aquaporin 1 (AQP1)

AQP-CHIP; CHIP28; CO; Channel-Forming Integral Protein,28kDa; Aquaporin-CHIP; Urine water channel; Water channel protein for red blood cells and kidney proximal tubule

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Aquaporin 1, Colton Blood Group (AQP1). No significant cross-reactivity or interference between Aquaporin 1, Colton Blood Group (AQP1) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Neuroscience Letters Aquaporins AQP1 and AQP4 in the cerebrospinal fluid of bacterial meningitis patients ScienceDirect: S0304394011012341
Cephalalgia Decreased levels of aquaporin-4 in the cerebrospinal fluid of patients with idiopathic intracranial hypertension Pubmed:26853804
Clin Neurol Neurosurg.  Cerebrospinal fluid levels of tumor necrosis factor alpha and aquaporin 1 in patients with mild cognitive impairment and idiopathic normal pressure hydrocephalus Pubmed:27155076
Journal of Neurotrauma Neuron-derived Plasma Exosome Proteins after Remote Traumatic Brain Injury Pubmed: 31441374
PLoS One Evaluation of aquaporins in the cerebrospinal fluid in patients with idiopathic normal pressure hydrocephalus 34597351
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