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ELISA Kit DIY Materials for Substance P (SP)

  • Product No.KSA393Hu11
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range12.35-1000pg/mL
  • Applicable Sensitivity5.24pg/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 716  US$ 1194  US$ 2149  US$ 4179  US$ 7164 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Substance P (SP). No significant cross-reactivity or interference between Substance P (SP) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Journal of Veterinary Internal Medicine A Potential Role for Substance P and Interleukin-6 in the Cerebrospinal Fluid of Cavalier King Charles Spaniels with Neuropathic Pain Pubmed: 23659719
Pharmacol Res Oral curcumin has anti-arthritic efficacy through somatostatin generation PubMed: 25836921
Applied Biological Chemistry Positive enhancement of Lactobacillus fermentum HY01 on intestinal movements of mice having constipation 10.1007/s13765-017-0327-3
CNS Neuroscience & Therapeutics  Direct activation of tachykinin receptors within baroreflex afferent pathway and neurocontrol of blood pressure regulation Pubmed:29900692
Journal of Veterinary Science Serum Levels of Neuropeptides in Cows with Left Abomasal Displacement Pubmed: 30562932
Respiratory Physiology & Neurobiology Effects of nanoparticles on Neuroinflammation in a Mouse Model of Asthma Pubmed: 31542455
Chinese Medicine Hejie Zhitong prescription promotes sleep and inhibits nociceptive transmission-associated neurotransmitter activity in a rodent migraine model Pubmed: 33014123
Medical Research Journal Changes of substance P, NGF and CGRP salivary levels among patients undergoing physical therapy
Int Urol Nephrol Evaluation of the causes affecting the development of pruritus in patients with peritoneal dialysis 34213714
J Chem Neuroanat Surfactant protein C is associated with perineuronal nets and shows age-dependent changes of brain content and hippocampal deposits in wildtype and 3xTg mice 34626771
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
SPA393Hu01 Synthetic Substance P (SP) Immunogen; Blocking Peptide.
PAA393Hu01 Polyclonal Antibody to Substance P (SP) WB; IHC; ICC; IP.
CEA393Hu ELISA Kit for Substance P (SP) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA393Hu CLIA Kit for Substance P (SP) Chemiluminescent immunoassay for Antigen Detection.
LMA393Hu Multiplex Assay Kit for Substance P (SP) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA393Hu11 ELISA Kit DIY Materials for Substance P (SP) Main materials for "Do It (ELISA Kit) Yourself".