ELISA Kit DIY Materials for Interleukin 8 (IL8)

CXCL8; AMCF-I; GCP1; K60; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP1; SCYB8; TSG1; B-ENAP; Neutrophil-Activating Protein 1; Granulocyte Chemotactic Protein 1

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Interleukin 8 (IL8).No significant cross-reactivity or interference between Interleukin 8 (IL8) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Reproductive Biology and Endocrinology Evidence that polymorphonuclear neutrophils infiltrate into the developing corpus luteum and promote angiogenesis with interleukin-8 in the cow Rbej: 14777827
Acta Otolaryngol Serial cytokine levels during wound healing in rabbit maxillary sinus mucosa PubMed: 19958244
Journal of Clinical Periodontology Experimental peri-implant mucositis at different implant surfaces. Pubmed: 24521508
Physiological Research Budesonide added to modified porcine surfactant Curosurf may additionally improve the lung functions in meconium aspiration syndrome. Pubmed: 2432969
Rheumatology International Serum levels of selected chemokines in systemic lupus erythematosus patients Pubmed: 22461186
J Med Life. Expression of interleukine-8 as an independent prognostic factor for sporadic colon cancer dissemination Pubmed:Pmc4197484
J Clin Periodontol. Experimental peri‐implant mucositis at different implant surfaces Pubmed:24521508
British Journal of Nutrition Grape seed extract supplementation attenuates the heat stress-induced responses of jejunum epithelial cells in Simmental?×?Qinchuan steers Pubmed:24846452
Theriogenology Inflammatory cytokine concentrations in uterine flush and serum samples from dairy cows with clinical or subclinical endometritis Theriojournal:Source
Anim Reprod Sci. Alteration in peripheral blood concentration of certain pro-inflammatory cytokines in cows developing retention of fetal membranes PubMed: 25851495
Медицина и здравоохранение ДИНАМИКА ЦИТОКИНОВОГО ПРОФИЛЯ В ОЦЕНКЕ ЭФФЕКТИВНОСТИ РАЗНЫХ МЕТОДОВ СТИМУЛЯЦИИ РЕПАРАТИВНОГО ОСТЕОГЕНЕЗА В ЭКСПЕРИМЕНТЕ Article: N
BMC Anesthesiology Hyperinflation deteriorates arterial oxygenation and lung injury in a rabbit model of ARDS with repeated open endotracheal suctioning PubMed: 25943099
J Breath Res Effects of different ventilation strategies on exhaled nitric oxide in geriatric abdominal surgery PubMed: 25719610
ARTICLE The Predictive Diagnostic and Prognostic Cut-off Values for Interleukin 8 in Patients with Meningitis in Egypt Profile: Mohmad_Mosaad
Veterinary Immunology and Immunopathology Neutrophil gene dynamics and plasma cytokine levels in dairy cattle during peri-implantation period Pubmed:27090626
Infection, Genetics and Evolution Infection of chicken bone marrow mononuclear cells with subgroup J avian leukosis virus inhibits dendritic cell differentiation and alters cytokine expression Pubmed:27349993
Asian Pacific Journal of Tropical Medicine Perfusion of gastrodin in abdominal aorta for alleviating spinal cord ischemia reperfusion injury science:S1995764516300955
Archives of Biological Sciences Diabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrol publication:299492265_Diabetes-induced_renal_failu
Journal of Agricultural Biotechnology Gln Attenuates LPS-induced Inflammatory Injury of Bos taurus Testis Sustentacular Cells 10610
journal of molecular and cellular cardiology Mutant DD genotype of NFKB1 gene is associated with the susceptibility and severity of coronary artery disease. pubmed:28088561
Physiological Research Intravenous dexamethasone attenuated inflammation and influenced apoptosis of lung cells in an experimental model of acute lung injury. pubmed:28006948
PLOS ONE Mechanical Ventilation Alters the Development of Staphylococcus aureus Pneumonia in Rabbit. pubmed:27391952
The Journal of Comenius University in Bratislava Effects of Conventional Mechanical Ventilation Performed by Two Neonatal Ventilators on the Lung Functions of Rabbits with Meconium-Induced Acute Lung Injury acm.2017.16.issue-3
Journal of Physiology and Pharmacology Effects of budesonide on the lung functions, inflammation and apoptosis in a saline-lavagemodel of acute lung injury. pubmed:28195073
中国药物警戒 小剂量阿奇霉素对稳定期老年慢性阻塞性肺疾病患者外周血内TNF-α、IL-8、CRP水平及肺功能的影响 Periodical_zgywjj201608004
Veterinary Immunology and Immunopathology Cytokine levels in colostrum and in foals' serum pre- and post-suckling pubmed:28242000
Journal of Cellular Physiology Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Models pubmed:27739057
European Journal of Pharmacology Cardioprotective time-window of Penehyclidine hydrochloride postconditioning: A rat study pubmed:28684235
PLoS One Linezolid and atorvastatin impact on pneumonia caused by Staphyloccocus aureus in rabbits with or without mechanical ventilation pubmed:29149185
Archives of Oral Biology The effect of restorative materials on cytokines in gingival crevicular fluid pubmed:28992599
Experimentelle periimplantäre Mukositis an unterschiedlichen Implantatoberflächen doi: 10.1111/jcpe.12240.
Molecular Medicine Reports CagA promotes proliferation and inhibits apoptosis of GES-1 cells by upregulating TRAF1/4-1BB pubmed:28627614
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY  Dexamethasone reduces serum level of IL-17 in Bleomycin-A5-induced rats model of pulmonary fibrosis pubmed:28608724
Biological Rhythm Research Alteration in some pro and anti-inflammatory cytokines associated with complete and incomplete gestation cycle of cows 10.1080/09291016.2017.1319636
Scientific Reports Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheets pubmed:28811665
Journal of Ultrasound in Medicine Inhibitory Effect of Low-Intensity Pulsed Ultrasound on the Expression of Lipopolysaccharide-Induced Inflammatory Factors in U937 Cells. pubmed:28600899
Veterinary Immunology and Immunopathology Integrated effect of seasons and lactation stages on the plasma inflammatory cytokines, function and receptor expression of milk neutrophils in Sahiwal (Bos indicus) cows. pubmed:28895861
Biomedicine hub Comparison of Polymyxin E and Polymyxin B as an Additive to Pulmonary Surfactant in Escherichia coli Pneumonia of Ventilated Neonatal Rabbits 10.1159/000475877
Anticancer Research Association Between Endothelial Progenitor Cells and Treatment Response in Non-Squamous Non-small Cell Lung Cancer Treated with Bevacizumab. pubmed:28982871
Chronic Respiratory Disease Inflammatory biomarkers and radiologic measurements in never-smokers with COPD: A cross-sectional study from the CODA cohort. pubmed:29117798
Inflammopharmacology Polar extract of protects cartilage homeostasis: possible mechanism of action Pubmed:29313174
Life Sciences Dexmedetomidine attenuates renal fibrosis via α2-adrenergic receptor-dependent inhibition of cellular senescence after renal ischemia/reperfusion Pubmed:29729264
Microbial Pathogenesis Pathogen-dependent modulation of milk neutrophils competence, plasma inflammatory cytokines and milk quality during intramammary infection of Sahiwal (Bos … Pubmed:29787791
Lasers in Medical Science Evaluation of the therapeutic effect of micro-plasma radio frequency on hypertrophic scars in rabbit ears Pubmed:30003425
Medical Science Monitor Rho Kinase Type 1 (ROCK1) Promotes Lipopolysaccharide-induced Inflammation in Corneal Epithelial Cells by Activating Toll-Like Receptor 4 (TLR4) … Pubmed:29804125
Journal of Veterinary Science Rifaximin anti‐inflammatory activity on bovine endometrium primary cell cultures: a preliminary study Pubmed:29984902
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