ELISA Kit DIY Materials for Interleukin 8 (IL8)

CXCL8; AMCF-I; GCP1; K60; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP1; SCYB8; TSG1; B-ENAP; Neutrophil-Activating Protein 1; Granulocyte Chemotactic Protein 1

  • Product No.KSA080Ca01
  • Organism SpeciesCanis familiaris; Canine (Dog) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.058ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 801  US$ 1335  US$ 2403  US$ 4673  US$ 8010 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Interleukin 8 (IL8). No significant cross-reactivity or interference between Interleukin 8 (IL8) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Reproductive Biology and Endocrinology Evidence that polymorphonuclear neutrophils infiltrate into the developing corpus luteum and promote angiogenesis with interleukin-8 in the cow Rbej: 14777827
Acta Otolaryngol Serial cytokine levels during wound healing in rabbit maxillary sinus mucosa PubMed: 19958244
Journal of Clinical Periodontology Experimental peri-implant mucositis at different implant surfaces. Pubmed: 24521508
Physiological Research Budesonide added to modified porcine surfactant Curosurf may additionally improve the lung functions in meconium aspiration syndrome. Pubmed: 2432969
Rheumatology International Serum levels of selected chemokines in systemic lupus erythematosus patients Pubmed: 22461186
J Med Life. Expression of interleukine-8 as an independent prognostic factor for sporadic colon cancer dissemination Pubmed:Pmc4197484
J Clin Periodontol. Experimental peri‐implant mucositis at different implant surfaces Pubmed:24521508
British Journal of Nutrition Grape seed extract supplementation attenuates the heat stress-induced responses of jejunum epithelial cells in Simmental?×?Qinchuan steers Pubmed:24846452
Theriogenology Inflammatory cytokine concentrations in uterine flush and serum samples from dairy cows with clinical or subclinical endometritis Theriojournal:Source
Anim Reprod Sci. Alteration in peripheral blood concentration of certain pro-inflammatory cytokines in cows developing retention of fetal membranes PubMed: 25851495
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ARTICLE The Predictive Diagnostic and Prognostic Cut-off Values for Interleukin 8 in Patients with Meningitis in Egypt Profile: Mohmad_Mosaad
Veterinary Immunology and Immunopathology Neutrophil gene dynamics and plasma cytokine levels in dairy cattle during peri-implantation period Pubmed:27090626
Infection, Genetics and Evolution Infection of chicken bone marrow mononuclear cells with subgroup J avian leukosis virus inhibits dendritic cell differentiation and alters cytokine expression Pubmed:27349993
Asian Pacific Journal of Tropical Medicine Perfusion of gastrodin in abdominal aorta for alleviating spinal cord ischemia reperfusion injury science:S1995764516300955
Archives of Biological Sciences Diabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrol publication:299492265_Diabetes-induced_renal_failu
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PLOS ONE Mechanical Ventilation Alters the Development of Staphylococcus aureus Pneumonia in Rabbit. pubmed:27391952
The Journal of Comenius University in Bratislava Effects of Conventional Mechanical Ventilation Performed by Two Neonatal Ventilators on the Lung Functions of Rabbits with Meconium-Induced Acute Lung Injury acm.2017.16.issue-3
Journal of Physiology and Pharmacology Effects of budesonide on the lung functions, inflammation and apoptosis in a saline-lavagemodel of acute lung injury. pubmed:28195073
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Veterinary Immunology and Immunopathology Cytokine levels in colostrum and in foals' serum pre- and post-suckling pubmed:28242000
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European Journal of Pharmacology Cardioprotective time-window of Penehyclidine hydrochloride postconditioning: A rat study pubmed:28684235
PLoS One Linezolid and atorvastatin impact on pneumonia caused by Staphyloccocus aureus in rabbits with or without mechanical ventilation pubmed:29149185
Archives of Oral Biology The effect of restorative materials on cytokines in gingival crevicular fluid pubmed:28992599
Experimentelle periimplantäre Mukositis an unterschiedlichen Implantatoberflächen doi: 10.1111/jcpe.12240.
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ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY  Dexamethasone reduces serum level of IL-17 in Bleomycin-A5-induced rats model of pulmonary fibrosis pubmed:28608724
Biological Rhythm Research Alteration in some pro and anti-inflammatory cytokines associated with complete and incomplete gestation cycle of cows 10.1080/09291016.2017.1319636
Scientific Reports Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheets pubmed:28811665
Journal of Ultrasound in Medicine Inhibitory Effect of Low-Intensity Pulsed Ultrasound on the Expression of Lipopolysaccharide-Induced Inflammatory Factors in U937 Cells. pubmed:28600899
Veterinary Immunology and Immunopathology Integrated effect of seasons and lactation stages on the plasma inflammatory cytokines, function and receptor expression of milk neutrophils in Sahiwal (Bos indicus) cows. pubmed:28895861
Biomedicine hub Comparison of Polymyxin E and Polymyxin B as an Additive to Pulmonary Surfactant in Escherichia coli Pneumonia of Ventilated Neonatal Rabbits 10.1159/000475877
Anticancer Research Association Between Endothelial Progenitor Cells and Treatment Response in Non-Squamous Non-small Cell Lung Cancer Treated with Bevacizumab. pubmed:28982871
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Medical Science Monitor Rho Kinase Type 1 (ROCK1) Promotes Lipopolysaccharide-induced Inflammation in Corneal Epithelial Cells by Activating Toll-Like Receptor 4 (TLR4) … Pubmed:29804125
Journal of Veterinary Science Rifaximin anti‐inflammatory activity on bovine endometrium primary cell cultures: a preliminary study Pubmed:29984902
Bioscience Reports N-Acetylcysteine inhalation improves pulmonary function in patients received Liver Transplantation. Doi: 10.1042/BSR20180858
Acta Medica Mediterranea Effects of Conventional Mechanical Ventilation Performed by Two Neonatal Ventilators on the Lung Functions of Rabbits with Meconium-Induced Acute Lung … Doi: 10.1515/acm-2016-0012
Journal of Dairy Science Metabolism and immune status during transition period influences the lactation performance in Zebu (Bos indicus () cows Pubmed: 17430930
Biomedicine & Pharmacotherapy Interleukin 8 targeted contrast echocardiography is effective to evaluate myocardial ischemia-reperfusion injury in the rabbits
Emerging Microbes & Infections Immunogenicity and protective efficacy against Treponema pallidum in New Zealand rabbits immunized with plasmid DNA encoding flagellin Pubmed: 30405111
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International Journal of Chronic Obstructive Pulmonary Disease Altered serum levels of type I collagen turnover indicators accompanied by IL-6 and IL-8 release in stable COPD Pubmed: 30655663
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Respiratory Research Synthetic surfactant with a recombinant surfactant protein C analogue improves lung function and attenuates inflammation in a model of acute respiratory … Pubmed: 31694668
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KSA080Ca01 ELISA Kit DIY Materials for Interleukin 8 (IL8) Main materials for "Do It (ELISA Kit) Yourself".