ELISA Kit DIY Materials for Interleukin 18 (IL18)

IGIF; IL-1g; IL1F4; IL1-F4; Interferon-Gamma-Inducing Factor; Interleukin-1 Family Member 4; Iboctadekin; Interleukin-1 Gamma

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Interleukin 18 (IL18). No significant cross-reactivity or interference between Interleukin 18 (IL18) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
International Immunopharmacology Curcumin immune-mediated and anti-apoptotic mechanisms protect against renal ischemia/reperfusion and distant organ induced injuries ScienceDirect: S1567576911001068
Atherosclerosis IL-18 overexpression promotes vascular inflammation and remodeling in a rat model of metabolic syndrome ScienceDirect: S0021915009006200
PLoS One. HIF-1α Is Essential for Effective PMN Bacterial Killing, Antimicrobial Peptide Production and Apoptosis in Pseudomonas aeruginosa Keratitis Plos: Source
临床儿科杂志 中性粒细胞明胶酶相关脂质运载蛋白、肾损伤分子1和白介素18 在诊断心肺分流术后急性肾损伤中的意义 Jcp-Sh:Source
Cell Death Dis. MicroRNA-30d regulates cardiomyocyte pyroptosis by directly targeting foxo3a in diabetic cardiomyopathy Pubmed:Pmc4237254
PLoS One. Cilostazol Renoprotective Effect: Modulation of PPAR-γ, NGAL, KIM-1 and IL-18 Underlies Its Novel Effect in a Model of Ischemia-Reperfusion Pubmed:24816434
J Korean Med Sci. Acute Kidney Injury after Using Contrast during Cardiac Catheterization in Children with Heart Disease Pubmed:25120320
PLOS ONE Protective Effect of Leaf Essential Oil from PubMed: 25794175
Fertil Steril Inflammosome in the human endometrium: further step in the evaluation of the “maternal side” PubMed: 26474737
United States Patent Application GLUCAN-ENCAPSULATED SIRNA FOR TREATING TYPE 2 DIABETES MELLITUS y2016:0138028.html
Front Pharmacol Poria Attenuates Idiosyncratic Liver Injury Induced by Polygoni Multiflori Radix Praeparata pubmed:27803670
Pharmacological reports The Role of PON-1, GR, IL-18, and oxLDL in Depression With and Without Posttraumatic Stress Disorder science:article
International Journal of Molecular Sciences PEDF Inhibits the Activation of NLRP3 Inflammasome in Hypoxia Cardiomyocytes through PEDF Receptor/Phospholipase A2 pubmed:27973457
Cellular Physiology and Biochemistry MiR-155 is Involved in Renal Ischemia-Reperfusion Injury via Direct Targeting of FoxO3a andRegulating Renal Tubular Cell Pyroptosis. pubmed:28006785
Journal of Food and Drug Analysis Anti-inflammatory effect of cinnamaldehyde and linalool from the leaf essential oil of Cinnamomum osmophloeum Kanehira in endotoxin-induced … S1021-9498(17)30081-9
Journal of Microbiology, Immunology and Infection Identification of a panel of serum protein markers in early stage of sepsis and its validation in a cohort of patients pubmed:28655573
Discovery and Validation of Immunological Biomarkers in Milk for Health Monitoring of Dairy Cows - Results from a Multiomics Approach doi:10.4172/2329-888X.1000182
Cellular Physiology and Biochemistry Dexmedetomidine Alleviates HyperoxiaInduced Acute Lung Injury via Inhibiting NLRP3 Inflammasome Activation pubmed:28873369
20 Melatonin prevents endothelial cell pyroptosis via regulation of long noncoding RNA MEG3/miR-223/NLRP3 axis pubmed:29024030
Pharmacological Reports The role of PON-1, GR, IL-18, and OxLDL in depression with and without posttraumatic stress disorder. pubmed:28623707
World journal of gastroenterology Hypothermic machine perfusion with metformin-University of Wisconsin solution for ex vivo preservation of standard and marginal liver grafts in a rat model. pubmed:29142469
Veterinary microbiology Secondary Haemophilus parasuis infection enhances highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) infection-mediated inflammatory responses. pubmed:28532803
International immunopharmacology Chronic dexamethasone treatment results in hippocampal neurons injury due to activate NLRP1 inflammasome in vitro pubmed:28605710
Journal of Neuroinflammation Chronic glucocorticoid exposure activates BK-NLRP1 signal involving in hippocampal neuron damage. pubmed:28732502
Journal of Neuroinflammation Unconjugated bilirubin induces pyroptosis in cultured rat cortical astrocytes Pubmed:29357878
Oxidative Medicine and Cellular Longevity The Effects of Sinapic Acid on the Development of Metabolic Disorders Induced by Estrogen Deficiency in Rats Pubmed:29967666
Journal of Translational Medicine Recurrent pregnancy loss is associated to leaky gut: a novel pathogenic model of endometrium inflammation? Pubmed:29665864
Chemico-Biological Interactions Nifuroxazide, a STAT3 inhibitor, mitigates inflammatory burden and protects against diabetes-induced nephropathy in rats Pubmed:29291386
Journal of Food and Drug Analysis Anti-inflammatory effect of cinnamaldehyde and linalool from the leaf essential oil of Cinnamomum osmophloeum Kanehira in endotoxin-induced mice Pubmed:29389558
International Journal of Molecular Medicine MCMV triggers ROS/NLRP3-associated inflammasome activation in the inner ear of mice and cultured spiral ganglion neurons, contributing to sensorineural hearing … Pubmed:29512778
Archives of Virology Genetic and immunogenicity analysis of porcine circovirus type 2 strains isolated in central China Pubmed:29305646
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KSA064Mu01 ELISA Kit DIY Materials for Interleukin 18 (IL18) Main materials for "Do It (ELISA Kit) Yourself".