High Sensitive ELISA Kit for Testosterone (Testo)

T; Testesteron; Testeronun

Specificity

This assay has high sensitivity and excellent specificity for detection of High Sensitive Testosterone (Testo).
No significant cross-reactivity or interference between High Sensitive Testosterone (Testo) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of High Sensitive Testosterone (Testo) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Testosterone (Testo) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 91-98 95
EDTA plasma(n=5) 80-96 86
heparin plasma(n=5) 97-105 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Testosterone (Testo) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Testosterone (Testo) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Testosterone (Testo) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-94% 80-90% 93-102% 82-98%
EDTA plasma(n=5) 78-99% 87-99% 78-91% 98-105%
heparin plasma(n=5) 96-103% 83-96% 94-105% 86-93%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

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Magazine Citations
The Tohoku Journal of Experimental Medicine Androgen Deprivation Induces Bladder Histological Abnormalities and Dysfunction via TGF-β in Orchiectomized Mature Rats Jstage: source
Plos one Insulin Receptor Substrate 2 Is Required for Testicular Development PubMed: PMC3669358
International Journal of Molecular and Cellular Medicine Central effects of camphor on GnRH and sexual hormones in male rat Pubmed: 24551777
Fertility and Sterility Growth differential factor-9 inhibits testosterone production in mouse theca interstitial cells Pubmed: 23954356
Urologia internationalis Effects of Tadalafil on Hemorrhagic Cystitis and Testicular Dysfunction Induced by Cyclophosphamide in Rats Pubmed: 24051982
International Braz J Urol The effect of sertraline, paroxetine, fluoxetine and escitalopram on testicular tissue and oxidative stress parameters in rats. Pubmed: 24642156
Journal of Materials Chemistry B Nanocomposites of bio-based hyperbranched polyurethane/funtionalized MWCNT as non-immunogenic, osteoconductive, biodegradable and biocompatible scaffolds in bone tissue engineering Rsc: Source
Biochemical and Biophysical Research Communications Critical function of RA-GEF-2/Rapgef6, a guanine nucleotide exchange factor for Rap1, in mouse spermatogenesis ScienceDirect: S0006291X14001843
PLoS One. Diets rich in saturated and polyunsaturated fatty acids induce morphological alterations in the rat ventral prostate Pubmed:Pmc4100933
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Physiology &amp; Behavior The impact of maternal consumption of cafeteria diet on reproductive function in the offspring Pubmed:24631302
Int Braz J Urol. The effect of Sertraline, Paroxetine, Fluoxetine and Escitalopram on testicular tissue and oxidative stress parameters in rats Pubmed:24642156
Environmental Research Perfluorooctane sulfonate effects on the reproductive axis in adult male rats Pubmed:25171141
Acta Cir Bras. Lard and/or canola oil-rich diets induce penile morphological alterations in a rat model. Pubmed:25185055
Genet Mol Biol. Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertility Pubmed:25505846
Turkish Journal of Urology Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapy Turkishjournalofurology:Source
Int J Clin Exp Med Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetes PubMed: 26379831
Int Braz J Urol Metabolic and Testicular Effects of the Long-Term Administration of Different High-Fat Diets in Adult Rats PubMed: 26200553
Advances in Environmental Biology Plasma Testosterone and Testis Histological Features of Mice Treated with Averrhoa Bilimbi, Cosmos Caudatus and Pereskia Bleo Ethanolic Extract Aensiweb: Aeb
international journal of environmental research and public health Elevated Serum Bisphenol A Level in Patients with Dilated Cardiomyopathy PubMed: 25996886
Environ Toxicol Effect on the H20 gene methylation of sperm and organs of offspring after chlorpyrifos‐methyl exposure during organogenesis period PubMed: 25782374
J Endocrinol Testosterone replacement attenuates mitochondrial damage in a rat model of myocardial infarction PubMed: 25770118
Acta Pharmaceutica Sinica B Preparation and evaluation of a phospholipid-based injectable gel for the long term delivery of leuprolide acetaterrh science:S2211383516301812
Plos One Association between Polycystic Ovary Syndrome and Cavia (Guinea pig )t Microbiota Pubmed:27093642
Theriogenology Expression of androgen-producing enzyme genes and testosterone concentration in AnCavia (Guinea pig )s and Nellore heifers with high and low ovarian follicle count Pubmed:26948295
PPAR Research Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction byUpreCavia (Guinea pig )lating PPARα Pubmed:27413362
TURKISH JOURNAL OF MEDICAL SCIENCES THE EFFECT OF KISSPEPTIN ON SPERMATOGENESIS AND APOPTOSIS IN RATS fileID:693211
Int J Mol Sci. Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice Pubmed:26771609
Scientific Reports Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus pubmed:27461373
Journal of Applied Life Sciences International Maternal Sleep Deprivation Alters Reproductive Capability of Male Offspring in Wistar Rats Akindele922016JALSI29782.pdf
Integrative Zoology Recovery of fertility in quinestrol-treated or diethylstilbestrol-treated mice: Implications for rodent management. pubmed:27611741
EBioMedicine. Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. pubmed:28336389
environmental pollution Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum. pubmed:27838061
PPAR Research Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction by Upregulating PPARα pubmed:27413362
Andrologia Effects of treatment with Hypoxis hemerocallidea extract on sexual behaviour and reproductiveparameters in male rats. pubmed:28000943
Turkish journal of medical sciences The effect of kisspeptin on spermatogenesis and apoptosis in rats. pubmed:28263511
Kafkas Universitesi Veteriner Fakultesi Dergisi The Angiogenic Effect of Testosterone Supplementation on Brain of Aged Mice. 13006045
Effect of dietary fish oil on mouse testosterone level and the distribution of eicosapentaenoic acid-containing phosphatidylcholine in testicular interstitium S2405580816300991
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