High Sensitive ELISA Kit for Testosterone (Testo)
T; Testesteron; Testeronun
- Product No.HEA458Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- Sample Typeserum, plasma and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range12.35-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 4.65pg/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 438
For more details, please contact local distributors! US$ 1971
For more details, please contact local distributors! US$ 3723
For more details, please contact local distributors! US$ 30660
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of High Sensitive Testosterone (Testo).
No significant cross-reactivity or interference between High Sensitive Testosterone (Testo) and analogues was observed.
Matrices listed below were spiked with certain level of High Sensitive Testosterone (Testo) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Testosterone (Testo) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Testosterone (Testo) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Testosterone (Testo) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Testosterone (Testo) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|The Tohoku Journal of Experimental Medicine||Androgen Deprivation Induces Bladder Histological Abnormalities and Dysfunction via TGF-β in Orchiectomized Mature Rats Jstage: source|
|Plos one||Insulin Receptor Substrate 2 Is Required for Testicular Development PubMed: PMC3669358|
|International Journal of Molecular and Cellular Medicine||Central effects of camphor on GnRH and sexual hormones in male rat Pubmed: 24551777|
|Fertility and Sterility||Growth differential factor-9 inhibits testosterone production in mouse theca interstitial cells Pubmed: 23954356|
|Urologia internationalis||Effects of Tadalafil on Hemorrhagic Cystitis and Testicular Dysfunction Induced by Cyclophosphamide in Rats Pubmed: 24051982|
|International Braz J Urol||The effect of sertraline, paroxetine, fluoxetine and escitalopram on testicular tissue and oxidative stress parameters in rats. Pubmed: 24642156|
|Journal of Materials Chemistry B||Nanocomposites of bio-based hyperbranched polyurethane/funtionalized MWCNT as non-immunogenic, osteoconductive, biodegradable and biocompatible scaffolds in bone tissue engineering Rsc: Source|
|Biochemical and Biophysical Research Communications||Critical function of RA-GEF-2/Rapgef6, a guanine nucleotide exchange factor for Rap1, in mouse spermatogenesis ScienceDirect: S0006291X14001843|
|PLoS One.||Diets rich in saturated and polyunsaturated fatty acids induce morphological alterations in the rat ventral prostate Pubmed:Pmc4100933|
|Reproductive Toxicology||Carbamazepine-exposure during gestation and lactation affects pubertal onset and spermatic parameters in male pubertal offspring Pubmed:24126190|
|Physiology & Behavior||The impact of maternal consumption of cafeteria diet on reproductive function in the offspring Pubmed:24631302|
|Int Braz J Urol.||The effect of Sertraline, Paroxetine, Fluoxetine and Escitalopram on testicular tissue and oxidative stress parameters in rats Pubmed:24642156|
|Environmental Research||Perfluorooctane sulfonate effects on the reproductive axis in adult male rats Pubmed:25171141|
|Acta Cir Bras.||Lard and/or canola oil-rich diets induce penile morphological alterations in a rat model. Pubmed:25185055|
|Genet Mol Biol.||Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertility Pubmed:25505846|
|Turkish Journal of Urology||Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapy Turkishjournalofurology:Source|
|Int J Clin Exp Med||Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetes PubMed: 26379831|
|Int Braz J Urol||Metabolic and Testicular Effects of the Long-Term Administration of Different High-Fat Diets in Adult Rats PubMed: 26200553|
|Advances in Environmental Biology||Plasma Testosterone and Testis Histological Features of Mice Treated with Averrhoa Bilimbi, Cosmos Caudatus and Pereskia Bleo Ethanolic Extract Aensiweb: Aeb|
|international journal of environmental research and public health||Elevated Serum Bisphenol A Level in Patients with Dilated Cardiomyopathy PubMed: 25996886|
|Environ Toxicol||Effect on the H20 gene methylation of sperm and organs of offspring after chlorpyrifos‐methyl exposure during organogenesis period PubMed: 25782374|
|J Endocrinol||Testosterone replacement attenuates mitochondrial damage in a rat model of myocardial infarction PubMed: 25770118|
|Acta Pharmaceutica Sinica B||Preparation and evaluation of a phospholipid-based injectable gel for the long term delivery of leuprolide acetaterrh science:S2211383516301812|
|Plos One||Association between Polycystic Ovary Syndrome and Cavia (Guinea pig )t Microbiota Pubmed:27093642|
|Theriogenology||Expression of androgen-producing enzyme genes and testosterone concentration in AnCavia (Guinea pig )s and Nellore heifers with high and low ovarian follicle count Pubmed:26948295|
|PPAR Research||Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction byUpreCavia (Guinea pig )lating PPARα Pubmed:27413362|
|TURKISH JOURNAL OF MEDICAL SCIENCES||THE EFFECT OF KISSPEPTIN ON SPERMATOGENESIS AND APOPTOSIS IN RATS fileID:693211|
|Int J Mol Sci.||Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice Pubmed:26771609|
|Scientific Reports||Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus pubmed:27461373|
|Journal of Applied Life Sciences International||Maternal Sleep Deprivation Alters Reproductive Capability of Male Offspring in Wistar Rats Akindele922016JALSI29782.pdf|
|Integrative Zoology||Recovery of fertility in quinestrol-treated or diethylstilbestrol-treated mice: Implications for rodent management. pubmed:27611741|
|EBioMedicine.||Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. pubmed:28336389|
|environmental pollution||Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum. pubmed:27838061|
|PPAR Research||Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction by Upregulating PPARα pubmed:27413362|
|Andrologia||Effects of treatment with Hypoxis hemerocallidea extract on sexual behaviour and reproductiveparameters in male rats. pubmed:28000943|
|Turkish journal of medical sciences||The effect of kisspeptin on spermatogenesis and apoptosis in rats. pubmed:28263511|
|Kafkas Universitesi Veteriner Fakultesi Dergisi||The Angiogenic Effect of Testosterone Supplementation on Brain of Aged Mice. 13006045|
|Effect of dietary fish oil on mouse testosterone level and the distribution of eicosapentaenoic acid-containing phosphatidylcholine in testicular interstitium S2405580816300991|
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