ELISA Kit for Testosterone (Testo)

T; Testesteron; Testeronun


This assay has high sensitivity and excellent specificity for detection of Testosterone (Testo).
No significant cross-reactivity or interference between Testosterone (Testo) and analogues was observed.


Matrices listed below were spiked with certain level of Testosterone (Testo) and the recovery rates were calculated by comparing the measured value to the expected amount of Testosterone (Testo) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 98-105 101
EDTA plasma(n=5) 98-105 101
heparin plasma(n=5) 98-105 101


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Testosterone (Testo) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Testosterone (Testo) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Testosterone (Testo) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-104% 88-96% 91-99% 80-98%
EDTA plasma(n=5) 87-98% 99-105% 92-101% 90-99%
heparin plasma(n=5) 80-91% 91-99% 81-91% 90-98%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.



Magazine Citations
The Tohoku Journal of Experimental Medicine Androgen Deprivation Induces Bladder Histological Abnormalities and Dysfunction via TGF-β in Orchiectomized Mature Rats Jstage: source
Plos one Insulin Receptor Substrate 2 Is Required for Testicular Development PubMed: PMC3669358
International Journal of Molecular and Cellular Medicine Central effects of camphor on GnRH and sexual hormones in male rat Pubmed: 24551777
Fertility and Sterility Growth differential factor-9 inhibits testosterone production in mouse theca interstitial cells Pubmed: 23954356
Urologia internationalis Effects of Tadalafil on Hemorrhagic Cystitis and Testicular Dysfunction Induced by Cyclophosphamide in Rats Pubmed: 24051982
International Braz J Urol The effect of sertraline, paroxetine, fluoxetine and escitalopram on testicular tissue and oxidative stress parameters in rats. Pubmed: 24642156
Journal of Materials Chemistry B Nanocomposites of bio-based hyperbranched polyurethane/funtionalized MWCNT as non-immunogenic, osteoconductive, biodegradable and biocompatible scaffolds in bone tissue engineering Rsc: Source
Biochemical and Biophysical Research Communications Critical function of RA-GEF-2/Rapgef6, a guanine nucleotide exchange factor for Rap1, in mouse spermatogenesis ScienceDirect: S0006291X14001843
PLoS One. Diets rich in saturated and polyunsaturated fatty acids induce morphological alterations in the rat ventral prostate Pubmed:Pmc4100933
Reproductive Toxicology Carbamazepine-exposure during gestation and lactation affects pubertal onset and spermatic parameters in male pubertal offspring Pubmed:24126190
Physiology &amp; Behavior The impact of maternal consumption of cafeteria diet on reproductive function in the offspring Pubmed:24631302
Int Braz J Urol. The effect of Sertraline, Paroxetine, Fluoxetine and Escitalopram on testicular tissue and oxidative stress parameters in rats Pubmed:24642156
Environmental Research Perfluorooctane sulfonate effects on the reproductive axis in adult male rats Pubmed:25171141
Acta Cir Bras. Lard and/or canola oil-rich diets induce penile morphological alterations in a rat model. Pubmed:25185055
Genet Mol Biol. Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertility Pubmed:25505846
Turkish Journal of Urology Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapy Turkishjournalofurology:Source
Int J Clin Exp Med Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetes PubMed: 26379831
Int Braz J Urol Metabolic and Testicular Effects of the Long-Term Administration of Different High-Fat Diets in Adult Rats PubMed: 26200553
Advances in Environmental Biology Plasma Testosterone and Testis Histological Features of Mice Treated with Averrhoa Bilimbi, Cosmos Caudatus and Pereskia Bleo Ethanolic Extract Aensiweb: Aeb
international journal of environmental research and public health Elevated Serum Bisphenol A Level in Patients with Dilated Cardiomyopathy PubMed: 25996886
Environ Toxicol Effect on the H20 gene methylation of sperm and organs of offspring after chlorpyrifos‐methyl exposure during organogenesis period PubMed: 25782374
J Endocrinol Testosterone replacement attenuates mitochondrial damage in a rat model of myocardial infarction PubMed: 25770118
Acta Pharmaceutica Sinica B Preparation and evaluation of a phospholipid-based injectable gel for the long term delivery of leuprolide acetaterrh science:S2211383516301812
Plos One Association between Polycystic Ovary Syndrome and Cavia (Guinea pig )t Microbiota Pubmed:27093642
Theriogenology Expression of androgen-producing enzyme genes and testosterone concentration in AnCavia (Guinea pig )s and Nellore heifers with high and low ovarian follicle count Pubmed:26948295
PPAR Research Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction byUpreCavia (Guinea pig )lating PPARα Pubmed:27413362
Int J Mol Sci. Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice Pubmed:26771609
Scientific Reports Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus pubmed:27461373
Journal of Applied Life Sciences International Maternal Sleep Deprivation Alters Reproductive Capability of Male Offspring in Wistar Rats Akindele922016JALSI29782.pdf
Integrative Zoology Recovery of fertility in quinestrol-treated or diethylstilbestrol-treated mice: Implications for rodent management. pubmed:27611741
33 Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. pubmed:28336389
environmental pollution Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum. pubmed:27838061
PPAR Research Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction by Upregulating PPARα pubmed:27413362
Andrologia Effects of treatment with Hypoxis hemerocallidea extract on sexual behaviour and reproductiveparameters in male rats. pubmed:28000943
Turkish journal of medical sciences The effect of kisspeptin on spermatogenesis and apoptosis in rats. pubmed:28263511
Kafkas Universitesi Veteriner Fakultesi Dergisi The Angiogenic Effect of Testosterone Supplementation on Brain of Aged Mice. 13006045
Effect of dietary fish oil on mouse testosterone level and the distribution of eicosapentaenoic acid-containing phosphatidylcholine in testicular interstitium S2405580816300991
Reproductive Sciences Abnormality of Klotho Signaling Is Involved in Polycystic Ovary Syndrome pubmed:28673204
Biomedicine & Pharmacotherapy Toxic effects of Tripterygium wilfordii Hook F on the reproductive system of adolescent male rats pubmed:28938525
Molecular and Cellular Endocrinology Testosterone deficiency prevents left ventricular contractility dysfunction after myocardial infarction pubmed:28606867
Ankara Üniv Vet Fak Derg The effect of testosterone supplementation on morphometry and VEGF expression level of thoracic aorta in aged mice DOI: 10.1501/Vetfak_0000002791
Acta histochemica Impaired steroidogenesis in the testis of leptin-deficient mice (ob/ob -/-) pubmed:28506466
DARU Journal of Pharmaceutical Sciences Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway pubmed:29020999
Environmental Pollution Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population Pubmed:29525625
Aquatic Toxicology Exposure to tris (1, 3-dichloro-2-propyl) phosphate for Two generations decreases fecundity of zebrafish at environmentally relevant concentrations Pubmed:29775925
Molecular Reproduction and Development  deficiency leads to testicular dysfunction and altered expression of genes involved in cell communication, chromatin reorganization, spermatogenesis, and … Pubmed:29411926
Journal of Ovarian Research DHEA-induced ovarian hyperfibrosis is mediated by TGF-β signaling pathway Pubmed:29321035
Medical Science Monitor Eicosapentaenoic Acid Improves Polycystic Ovary Syndrome in Rats via Sterol Regulatory Element-Binding Protein 1 (SREBP-1)/Toll-Like Receptor 4 (TLR4) … Pubmed:29627845
Oncology Letters Prostaglandin E2 triggers cytochrome P450 17α hydroxylase overexpression via signal transducer and activator of transcription 3 phosphorylation and promotes … 10.3892:ol.2018.9165
Biomedicine & Pharmacotherapy Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neurons Doi: 10.1016/j.biopha.2018.08.135
Asian Journal of Andrology Resveratrol attenuates metabolic, sperm, and testicular changes in adult Wistar rats fed a diet rich in lipids and simple carbohydrates Pubmed: 30198494
第三军医大学学报 基因敲除损伤小鼠睾丸睾酮产生与生精功能
Stem Cells Translational Medicine Protective Effects of Uncultured Adipose‐Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion‐Detorsion in Rats Pubmed: 30569668
Brazilian Journal of Pharmaceutical Science Demographic, clinical and lifestyle predictors for severity of erectile dysfunction and biomarkers level in Malaysian patients
Journal of Endocrinology Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome Pubmed: 29535146
Journal of Cellular Physiology Brain‐derived neurotrophic factor: A steroidogenic regulator of Leydig cells Pubmed: 30628054
Experimental and Therapeutic Medicine Tanshinone IIA attenuates estradiol‑induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary
Evidence-Based Complementary and Alternative Medicine iTRAQ-Based Proteomics to Reveal the Mechanism of Hypothalamus in Kidney-Yin Deficiency Rats Induced by Levothyroxine
International Journal of Reproductive Biomedicine The therapeutic potential of amifostine on cyclophosphamide-induced testicular dysfunction in rats: An experimental study.
Kafkas Universitesi Veteriner Fakultesi Dergisi The Effect of Testosterone Supplementation on Capillar Morphometry and VEGF Expression Level of the Heart in Aged Mice
Journal of Cellular and Molecular Medicine Cyclophilin D participates in the inhibitory effect of high‐fat diet on the expression of steroidogenic acute regulatory protein Pubmed: 31373170
Journal of neuroscience research Revisiting the relationships of 2D:4D with androgen receptor (AR) gene and current testosterone levels: Replication study and meta‐analyses Pubmed: 31359506
Andrologia Sperm parameters quality and reproductive effects of methanolic extract of Alchornea cordifolia leaves on senescent male rats Pubmed: 31353623
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