Rat Model for Acute Lung Injury (ALI)
Acute Respiratory Distress Syndrome
- Product No.DSI524Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Prototype SpeciesHuman
- SourceLipopolysaccharide (LPS) induced
- Model Animal StrainsWistar rats (SPF level), male, body weight 180g~220g.
- Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group and Test drug group (three doses)
- Modeling Period24hours
- Modeling MethodRats are fixed into box, wipe rat tail vein with alcohol, press and hold the tail vein at 1/3, inject LPS solution (dissolved in physiological saline, dose of 10 mg/kg). The same operation for rats in the normal control group, but the saline is injected into the tail vein.
- ApplicationsLPS induced Acute Lung Injury Model has a practical value in the research of drug screening and pathogenesis.
- DownloadInstruction Manual
For more details, please contact local distributors!
1.Observation of lung injury:
Lung injury can be seen with the naked eye, large area of bleeding and exudation, can directly reflect the degree of lung injury.
2.Lung wet/dry weight ratio:
Lung wet/dry weight ratio is a direct indicator of pulmonary edema, and it is also a sensitive indicator of the severity of lung injury. After the occurrence acute lung injury (ALI), a large amount of fluid to the alveolar and interstitial fluid, leading to increased weight of the lungs, while the dry weight of the lung is not affected.
After killing the rats, cut trachea from whole lung and wet weight, dry the lung on 65℃ for 24hrs, and then calculate lung wet/ dry weight ratio.
Take the left lung 4% poly formaldehyde fixed, and then the normal lung tissue dehydration, paraffin embedded, sliced (thickness of 5μm), HE staining.
HE staining of lung tissue can directly response the degree of lung injury, ALI tissue pathology mainly in alveolar neutrophils and red blood cells exudation, alveolar wall transparent membrane formation. HE staining after can be in inflammatory cells was observed under low magnification infiltration, edema and injury.
At high magnification, the alveolar structure can be identified, and the degree of lung injury can be assessed and evaluated.
Scoring method: take 6 field of vision for bleeding and edema score.
0 points: no damage
1 points: <25% damage area
2 points: 25%~50% damage area
3 points: 50%~75% damage area
4 points: >75% damage area
Anatomy of left lobectomy trachea, bronchial intubation, precooled sterile normal saline 1 ml, bronchoalveolar lavage 3 times, recycling bronchial alveolar lavage fluid (BALF) to a sterile centrifuge tube, 1500 R/min, 10 mins, collected and stored on 80℃, using ELISA kit for assay of TNF-a, IL-6, IL-1 beta and other cytokins in BALF.
SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison, P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.
- Tissue/Sections Customized Service
- Serums Customized Service
- Immunohistochemistry (IHC) Experiment Service
- Small Animal In Vivo Imaging Experiment Service
- Small Animal Micro CT Imaging Experiment Service
- Small Animal MRI Imaging Experiment Service
- Small Animal Ultrasound Imaging Experiment Service
- Transmission Electron Microscopy (TEM) Experiment Service
- Scanning Electron Microscope (SEM) Experiment Service
- Learning and Memory Behavioral Experiment Service
- Anxiety and Depression Behavioral Experiment Service
- Drug Addiction Behavioral Experiment Service
- Pain Behavioral Experiment Service
- Neuropsychiatric Disorder Behavioral Experiment Service
- Fatigue Behavioral Experiment Service
|Catalog No.||Related products for research use of Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
|DSI524Ra01||Rat Model for Acute Lung Injury (ALI)||LPS induced Acute Lung Injury Model has a practical value in the research of drug screening and pathogenesis.|