ELISA Kit for Neurofilament, Light Polypeptide (NEFL)

CMT1F; CMT2E; NF-L; NF68; NFL; 68 kDa neurofilament protein; Neurofilament triplet L protein

Specificity

This assay has high sensitivity and excellent specificity for detection of Neurofilament, Light Polypeptide (NEFL).
No significant cross-reactivity or interference between Neurofilament, Light Polypeptide (NEFL) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Neurofilament, Light Polypeptide (NEFL) and the recovery rates were calculated by comparing the measured value to the expected amount of Neurofilament, Light Polypeptide (NEFL) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 91-104 97
EDTA plasma(n=5) 87-94 91
heparin plasma(n=5) 90-99 93

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neurofilament, Light Polypeptide (NEFL) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neurofilament, Light Polypeptide (NEFL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Neurofilament, Light Polypeptide (NEFL) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-99% 82-92% 83-93% 95-102%
EDTA plasma(n=5) 78-92% 80-96% 80-88% 80-98%
heparin plasma(n=5) 93-103% 95-102% 93-101% 87-95%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Acta Neurologica Scandinavica Effect of carotid endarterectomy on brain damage markers Pubmed:27126899
Acta Neurologica Scandinavica Effect of carotid endarterectomy on brain damage markers. pubmed:27126899
journal of neuroimmunology Cytokines and biological markers in autoimmune GFAP astrocytopathy: The potential role for pathogenesis and therapeutic implications Pubmed: 31254929
Cerebellum Neurofilament Light Chain Is a Biomarker of Neurodegeneration in Ataxia Telangiectasia 33893614
Medicine (Baltimore) Phase 1b dose-escalation, safety, and pharmacokinetic study of IC14, a monoclonal antibody against CD14, for the treatment of amyotrophic lateral sclerosis 34678870
J Clin Med Fatty Acid-Binding Protein 7 (FABP-7), Glutamic Acid and Neurofilament Light Chain (NFL) as Potential Markers of Neurodegenerative Disorders in Psoriatic Patients … Pubmed:35566558
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
CEE038Ra ELISA Kit for Neurofilament, Light Polypeptide (NEFL) Enzyme-linked immunosorbent assay for Antigen Detection.
LME038Ra Multiplex Assay Kit for Neurofilament, Light Polypeptide (NEFL) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.