ELISA Kit for Kisspeptin 1 (KISS1)
Metastin; KiSS-1 Metastasis-Suppressor
- Product No.CEC559Bo
- Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
- Sample TypeSerum, plasma and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range12.35-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 5.51pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Kisspeptin 1 (KISS1).
No significant cross-reactivity or interference between Kisspeptin 1 (KISS1) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Kisspeptin 1 (KISS1) and the recovery rates were calculated by comparing the measured value to the expected amount of Kisspeptin 1 (KISS1) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kisspeptin 1 (KISS1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kisspeptin 1 (KISS1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Kisspeptin 1 (KISS1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Reagent Diluent||1×300µL||Stop Solution||1×6mL|
|TMB Substrate||1×9mL||Instruction manual||1|
|Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Mol Endocrinol.||LIM-homeodomain transcription factor Isl-1 mediates kisspeptin's effect on insulin secretion in mice. Pubmed:24956377|
|临床儿科杂志||双酚A 与女童性早熟的关系 Fileup: Pdf|
|J Pediatr Endocrinol Metab||The relation of urinary bisphenol A with kisspeptin in girls diagnosed with central precocious puberty and premature thelarche PubMed: 26565542|
|Archives of Physiology and Biochemistry||Circulating levels of leptin, nesfatin-1 and kisspeptin in postmenopausal obese women Pubmed:27011110|
|International Journal of Endocrinology||The Changes of Serum Leptin and Kisspeptin Levels in Chinese Children and Adolescents in Different Pubertal Stages pubmed:27990162|
|Journal of Clinical Research in Pediatric Endocrinology||Basal Serum Neurokinin B Levels in Differentiating Idiopathic Central Precocious Puberty from Premature Thelarche. pubmed:28008860|
|BioMed Research International||Kisspeptin Protein in Seminal Plasma Is Positively Associated with Semen Quality: Results from the MARHCS Study in Chongqing, China|
|OBESITY SURGERY||Lack of Improvement of Sperm Characteristics in Obese Males After Obesity Surgery Despite the Beneficial Changes Observed in Reproductive Hormones Pubmed: 30806913|
|Catalog No.||Related products for research use of Bos taurus; Bovine (Cattle) Organism species||Applications (RESEARCH USE ONLY!)|
|CEC559Bo||ELISA Kit for Kisspeptin 1 (KISS1)||Enzyme-linked immunosorbent assay for Antigen Detection.|