ELISA Kit for Cathepsin D (CTSD)
CPSD; CLN10; Lysosomal Aspartyl Protease; Ceroid-Lipofuscinosis,Neuronal 10
- Product No.CEB280Bo
- Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
- Sample TypeSerum, plasma and other biological fluids.
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range2.47-200ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.88ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Cathepsin D (CTSD).
No significant cross-reactivity or interference between Cathepsin D (CTSD) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Cathepsin D (CTSD) and the recovery rates were calculated by comparing the measured value to the expected amount of Cathepsin D (CTSD) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cathepsin D (CTSD) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cathepsin D (CTSD) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cathepsin D (CTSD) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Molecular Immunology||Cathepsin D is released after severe tissue trauma in vivo and is capable of generating C5a in vitro ScienceDirect: S0161589011008297|
|21||Plasma Cathepsin D Levels: A Novel Tool to Predict Pediatric Hepatic Inflammation PubMed: 25732418|
|Scientific Reportes||Plasma cathepsin D correlates with histological classifications of fatty liver disease in adults and responds to intervention pubmed:27922112|
|Biomedicine & pharmacotherapy||Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanisms pubmed:28061403|
|JGH OPEN||Limited applicability of cathepsin D for the diagnosis and monitoring of non‐alcoholic steatohepatitis|
|Diabetologia||Plasma cathepsin D activity is negatively associated with hepatic insulin sensitivity in overweight and obese humans Pubmed: 31690989|
|Antioxidants||Iron and Advanced Glycation End Products: Emerging Role of Iron in Androgen Deficiency in Obesity Pubmed: 32235809|
|Catalog No.||Related products for research use of Bos taurus; Bovine (Cattle) Organism species||Applications (RESEARCH USE ONLY!)|
|RPB280Bo01||Recombinant Cathepsin D (CTSD)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAB280Bo01||Polyclonal Antibody to Cathepsin D (CTSD)||WB; IHC; ICC; IP.|
|MAB280Bo21||Monoclonal Antibody to Cathepsin D (CTSD)||WB; IHC; ICC; IP.|
|SEB280Bo||ELISA Kit for Cathepsin D (CTSD)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|CEB280Bo||ELISA Kit for Cathepsin D (CTSD)||Enzyme-linked immunosorbent assay for Antigen Detection.|