ELISA Kit for Peptide YY (PYY)

PYY1; Peptide tyrosine tyrosine

Specificity

This assay has high sensitivity and excellent specificity for detection of Peptide YY (PYY).
No significant cross-reactivity or interference between Peptide YY (PYY) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Peptide YY (PYY) and the recovery rates were calculated by comparing the measured value to the expected amount of Peptide YY (PYY) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 92-101 96
EDTA plasma(n=5) 78-93 87
heparin plasma(n=5) 78-94 87

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peptide YY (PYY) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peptide YY (PYY) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Peptide YY (PYY) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-97% 88-95% 97-105% 87-95%
EDTA plasma(n=5) 90-102% 80-95% 79-102% 83-102%
heparin plasma(n=5) 85-104% 96-105% 93-101% 94-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1 Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Reagent Diluent 1×300µL Stop Solution 1×6mL
TMB Substrate 1×9mL Instruction manual 1
Wash Buffer (30 × concentrate) 1×20mL

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

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Magazine Citations
International Journal of Sport Studies The Effects of Concurrent Resistance and Endurance Exercise on Hunger Feelings and PYY in Obese Men Com:Source
Annals of Applied Sport Science Effect of an Acute Incremental Exercise on Plasma Peptide YY, Neuropeptide Y and IGF-1 Concentrations in Young Athletes Aassjournal:Source
Biomedical Reports Calprotectin in serum and zonulin in serum and feces are elevated after introduction of a diet with lower carbohydrate content and higher fiber, fat and protein contents. pubmed:28413639
Acta Diabetologica Effect of lifestyle improvement program on the biomarkersof adiposity, inflammation and gut hormones in overweight/obese Asian Indians with prediabetes pubmed:28620678
Biomedical Reports Calprotectin in serum and zonulin in serum and feces are elevated after introduction of a diet with lower carbohydrate content and higher fiber, fat and protein contents 10.3892/br.2017.865
Journal of Diabetes & Metabolism High Fiber Fat and Protein Contents Lead to Increased Satiety Reduced Sweet Cravings and Decreased Gastrointestinal Symptoms Independently of Anthropometric Hormonal and Metabolic Factors 10.4172/2155-6156.1000733 
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