ELISA Kit for Albumin (ALB)
- Product No.CEB028Ga
- Organism SpeciesChicken (Gallus) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range1.23-100ug/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.49ug/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 722
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For more details, please contact local distributors! US$ 50540
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This assay has high sensitivity and excellent specificity for detection of Albumin (ALB).
No significant cross-reactivity or interference between Albumin (ALB) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Albumin (ALB) and the recovery rates were calculated by comparing the measured value to the expected amount of Albumin (ALB) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Albumin (ALB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Albumin (ALB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Albumin (ALB) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
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|Liver International||Albumin administration protects against bilirubin-induced auditory brainstem dysfunction in Gunn rat pups Onlinelibrary: liv.12219|
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|Mol Cell Biochem||The wnt/β-catenin signaling pathway participates in rhein ameliorating kidney injury in DN mice PubMed: 26346164|
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|Experimental and molecular pathology||Alteration of Connexin43 expression in a rat model of obesity-related glomerulopathy. pubmed:29246788|
|Inflammation||Resolvin D1 Promotes SIRT1 Expression to Counteract the Activation of STAT3 and NF-κB in Mice with Septic-Associated Lung Injury Pubmed:30014231|
|Journal of Developmental Origins of Health and Disease||Maternal low-quality protein diet exerts sex-specific effects on plasma amino acid profile and alters hepatic expression of methyltransferases in adult rat offspring Pubmed:29582727|
|Medicine||Electret filter collects more exhaled albumin than glass condenser: A method comparison based on human study Pubmed:29384875|
|Stem Cell Research & Therapy||Therapeutic efficiency of human amniotic epithelial stem cell-derived functional hepatocyte-like cells in mice with acute hepatic failure Pubmed: 30463600|
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|biomedicine & pharmacotherapy||Treatment with 3, 4-dihydroxyphenylethyl alcohol glycoside ameliorates sepsis-induced ALI in mice by reducing inflammation and regulating M1 polarization Pubmed: 31146107|
|biochemical and biophysical research communications||Anti-proteinuria effect of antibody against ANGPTL3 coil-coiled domain on adriamycin-induced nephropathy in mice Pubmed: 31256934|
|Diabetes, Metabolic Syndrome and Obesity||A small molecule inhibitor MCC950 ameliorates kidney injury in diabetic nephropathy by inhibiting NLRP3 inflammasome activation Pubmed: 31447572|
|Molecular and Cellular Endocrinology||Diiodothyronines regulate metabolic homeostasis in primary human hepatocytes by modulating mTORC1 and mTORC2 activity Pubmed: 31580898|
|BMC Veterinary Research||Preliminary study on the effects of enrofloxacin, flunixin meglumine and pegbovigrastim on Mycoplasma bovis pneumonia Pubmed: 31655595|
|International Immunopharmacology||Ghrelin alleviates traumatic brain injury-induced acute lung injury through pyroptosis/NF-κB pathway Pubmed: 31918060|
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|Catalog No.||Related products for research use of Chicken (Gallus) Organism species||Applications (RESEARCH USE ONLY!)|
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