ELISA Kit for Albumin (ALB)

Serum albumin

Specificity

This assay has high sensitivity and excellent specificity for detection of Albumin (ALB).
No significant cross-reactivity or interference between Albumin (ALB) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Albumin (ALB) and the recovery rates were calculated by comparing the measured value to the expected amount of Albumin (ALB) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 93-101 97
EDTA plasma(n=5) 80-101 94
heparin plasma(n=5) 96-105 102

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Albumin (ALB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Albumin (ALB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Albumin (ALB) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-103% 82-97% 89-102% 78-96%
EDTA plasma(n=5) 87-101% 93-104% 79-93% 96-104%
heparin plasma(n=5) 80-101% 96-104% 80-97% 78-96%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
PLoS One Simvastatin Reduces Endotoxin-Induced Acute Lung Injury by Decreasing Neutrophil Recruitment and Radical Formation PubMed: 22701728
Liver International Albumin administration protects against bilirubin-induced auditory brainstem dysfunction in Gunn rat pups Onlinelibrary: liv.12219
Journal of Infectious Diseases Adjunctive corticosteroid therapy improves lung immunopathology and survival during severe secondary pneumococcal pneumonia in mice Pubmed: 24273183
Toxins (Basel). Increased Circulating Levels of Vitamin D Binding Protein in MS Patients Pubmed:25590278
Mol Cell Biochem The wnt/β-catenin signaling pathway participates in rhein ameliorating kidney injury in DN mice PubMed: 26346164
PLoS One Collecting Protein Biomarkers in Breath Using Electret Filters: A Preliminary Method on New Technical Model and Human Study Pubmed:26934615
Journal of Biomaterials and Tissue Engineering 3D Printing of Differentiated Bone Marrow Mesenchymal Cells as a New Method for Liver Tissue Engineering art00007
Experimental and molecular pathology Alteration of Connexin43 expression in a rat model of obesity-related glomerulopathy. pubmed:29246788
Inflammation Resolvin D1 Promotes SIRT1 Expression to Counteract the Activation of STAT3 and NF-κB in Mice with Septic-Associated Lung Injury Pubmed:30014231
Journal of Developmental Origins of Health and Disease Maternal low-quality protein diet exerts sex-specific effects on plasma amino acid profile and alters hepatic expression of methyltransferases in adult rat offspring Pubmed:29582727
Medicine Electret filter collects more exhaled albumin than glass condenser: A method comparison based on human study Pubmed:29384875
Catalog No. Related products for research use of Chicken (Gallus) Organism species Applications (RESEARCH USE ONLY!)
CEB028Ga ELISA Kit for Albumin (ALB) Enzyme-linked immunosorbent assay for Antigen Detection.