ELISA Kit for Neuropeptide Y (NPY)

NP-Y; PYY4; CPON; Pro-neuropeptide Y; Neuropeptide tyrosine; C-flanking peptide of NPY


This assay has high sensitivity and excellent specificity for detection of Neuropeptide Y (NPY).
No significant cross-reactivity or interference between Neuropeptide Y (NPY) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Neuropeptide Y (NPY) and the recovery rates were calculated by comparing the measured value to the expected amount of Neuropeptide Y (NPY) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 96-105 102
EDTA plasma(n=5) 93-101 96
heparin plasma(n=5) 87-101 92


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuropeptide Y (NPY) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuropeptide Y (NPY) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Neuropeptide Y (NPY) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-97% 84-101% 88-96% 93-105%
EDTA plasma(n=5) 94-102% 95-104% 89-103% 88-102%
heparin plasma(n=5) 95-105% 87-94% 82-103% 78-89%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1 Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Reagent Diluent 1×300µL Stop Solution 1×6mL
TMB Substrate 1×9mL Instruction manual 1
Wash Buffer (30 × concentrate) 1×20mL

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.



Magazine Citations
Hemodialysis International Is there any interaction of resistin and adiponectin levels with protein‐energy wasting among patients with chronic kidney disease Onlinelibrary: hdi.12072
Journal of Pediatric Endocrinology and Metabolism Changes in growth pattern, leptin ghrelin and neuropeptide Y levels after adenotonsillectomy in prepubertal children Degruyter: Source
Ren Fail. The role of adipocytokines on depressive symptoms of patients with chronic kidney disease Pubmed: 23879728
Coronary Artery Disease The relationship of plasma neuropeptide Y levels with coronary collateral development. Pubmed: 24077326
Coronary Artery Disease The relationship of plasma neuropeptide Y levels with coronary collateral development Lww:Source
Toxicology Letters Possible role of serotonin and neuropeptide Y on the disruption of the reproductive axis activity by perfluorooctane sulfonate Pubmed:25623392
J Neurosurg Anesthesiol High Plasma Levels of Neuropeptide Y Correlate With Good Clinical Outcome But are not Correlated to Cerebral Blood Flow or Vasospasm After Subarachnoid Hemorrhage PubMed: 25955865
Journal of Diabetes Research High Intensity Aerobic Exercise Training Improves Deficits of Cardiovascular Autonomic Function in a Rat Model of Type 1 Diabetes Mellitus with Moderate … Pubmed:26885531
Archives of Gynecology and Obstetrics Binge eating and biochemical markers of appetite in new users of the contraceptive depot medroxyprogesterone acetate pubmed:27604241
basic & clinical pharmacology & toxicology Effects of Di-isononyl Phthalate on Neuropeptide Y Expression in Differentiating Human Neuronal Cells. pubmed:27625336
Acta Neurobiologiae Experimentalis Aberrant changes of somatostatin and neuropeptide Y in brain of a genetic rat model for epilepsy: tremor rat pubmed:27685769
Cardiovascular research Nur77 protects against adverse cardiac remodelling by limiting Neuropeptide Y signalling in the sympathoadrenal-cardiac axis Pubmed:29850786
Journal of Periodontology Salivary neuropeptides, stress, and periodontitis Pubmed:28914594
Neuropeptides Mechanisms of sustained long-term weight loss after RYGB: α-MSH is a key factor Pubmed:29685637
Pharmacology Melatonin Induces Osteoblastic Differentiation of Mesenchymal Stem Cells and Promotes Fracture Healing in a Rat Model of Femoral Fracture via Neuropeptide Y … Pubmed: 30227410
Marmara Medical Journal Investigation of the relationship between insulin resistance and neuropeptide Y levels in polycystic ovary syndrome
Marmara Medical Journal Investigation of the relationship between insulin resistance and neuropeptide Y levels in polycystic ovary syndrome.
neurochemical research Sevoflurane Induces Hippocampal Neuronal Apoptosis by Altering the Level of Neuropeptide Y in Neonatal Rats. Pubmed: 32378074
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