ELISA Kit for Amyloid Beta Peptide 1-40 (Ab1-40)
- Product No.CEA864Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range12.35-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 4.58pg/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 630
For more details, please contact local distributors! US$ 2835
For more details, please contact local distributors! US$ 5355
For more details, please contact local distributors! US$ 44100
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Amyloid Beta Peptide 1-40 (Ab1-40).
No significant cross-reactivity or interference between Amyloid Beta Peptide 1-40 (Ab1-40) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Amyloid Beta Peptide 1-40 (Ab1-40) and the recovery rates were calculated by comparing the measured value to the expected amount of Amyloid Beta Peptide 1-40 (Ab1-40) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amyloid Beta Peptide 1-40 (Ab1-40) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amyloid Beta Peptide 1-40 (Ab1-40) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Amyloid Beta Peptide 1-40 (Ab1-40) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Reagent Diluent||1×300µL||Stop Solution||1×6mL|
|TMB Substrate||1×9mL||Instruction manual||1|
|Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|PLoS One||Metabolites of Cerebellar Neurons and Hippocampal Neurons Play Opposite Roles in Pathogenesis of Alzheimer's Disease PubMed: PMC2677455|
|Biochemical and Biophysical Research Communications||Antagonist of peroxisome proliferator-activated receptor γ induces cerebellar amyloid-β levels and motor dysfunction in APP/PS1 transgenic mice ScienceDirect: S0006291X09008468|
|Electromagnetic Biology and Medicine||Effect of 900 MHz Radio Frequency Radiation on Beta Amyloid Protein, Protein Carbonyl, and Malondialdehyde in the Brain Informa: 15368378.2011.624654|
|Electromagnetic Biology and Medicine||Do 100- and 500-μT ELF magnetic fields alter beta-amyloid protein, protein carbonyl and malondialdehyde in rat brains? PubMed: 23324065|
|Indian Journal of Experimental Biology||Intranasal administration of insulin lowers amyloid-levels in rat model of diabetes. PubMed: 22279939|
|European Journal of Pharmacology||The γ-secretase inhibitor 2-[(1R)-1-[(4-chlorophenyl)sulfonyl](2,5-difluorophenyl) amino]ethyl-5-fluorobenzenebutanoic acid (BMS-299897) alleviates Aβ1-42 seeding and short-term memory deficits in the Aβ25-35 mouse model of Alzheimer's disease. ScienceDirect: S0014299912009028|
|Neuropsychopharmacology||Blockade of Tau Hyperphosphorylation and Aβ1–42 Generation by the Aminotetrahydrofuran Derivative ANAVEX2-73, a Mixed Muscarinic and σ1 Receptor Agonist, in a Nontransgenic Mouse Model of Alzheimer’s Disease Pubmed: 23493042|
|世界科技研究与发展(World Sci-tech R & D)||慢病毒介导的ＣＢＳ基因过表达对原代皮质神经元 Globesci:Source|
|Neurochemical research||Effects of Aluminium on β-Amyloid (1–42) and Secretases (APP-Cleaving Enzymes) in Rat Brain Pubmed:24792732|
|Neurochem Int||Geniposide attenuates insulin-deficiency-induced acceleration of β-amyloidosis in an APP/PS1 transgenic model of Alzheimer's disease PubMed: 25882165|
|Journal of Alzheimer’s Parkinsonism & Dementia||The Potential Effect of Epigallocatechin-3-Gallate alone or in Combination with Vitamin E and Selenium on Alzheimer's Disease Induced by Aluminum in Rats open-access:|
|Am J Alzheimers Dis Other Demen.||Tong Luo Jiu Nao, a Chinese Medicine Formula, Reduces Inflammatory Stress in a Mouse Model of Alzheimer's Disease Pubmed:26993489|
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