ELISA Kit for Amyloid Beta Peptide 1-40 (Ab1-40)


This assay has high sensitivity and excellent specificity for detection of Amyloid Beta Peptide 1-40 (Ab1-40).
No significant cross-reactivity or interference between Amyloid Beta Peptide 1-40 (Ab1-40) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Amyloid Beta Peptide 1-40 (Ab1-40) and the recovery rates were calculated by comparing the measured value to the expected amount of Amyloid Beta Peptide 1-40 (Ab1-40) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 84-103 98
EDTA plasma(n=5) 80-101 86
heparin plasma(n=5) 94-101 97


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amyloid Beta Peptide 1-40 (Ab1-40) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amyloid Beta Peptide 1-40 (Ab1-40) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Amyloid Beta Peptide 1-40 (Ab1-40) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-101% 78-91% 89-96% 80-90%
EDTA plasma(n=5) 85-102% 96-104% 85-96% 78-101%
heparin plasma(n=5) 88-95% 78-94% 86-93% 98-105%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.



Magazine Citations
PLoS One Metabolites of Cerebellar Neurons and Hippocampal Neurons Play Opposite Roles in Pathogenesis of Alzheimer's Disease PubMed: PMC2677455
Biochemical and Biophysical Research Communications Antagonist of peroxisome proliferator-activated receptor γ induces cerebellar amyloid-β levels and motor dysfunction in APP/PS1 transgenic mice ScienceDirect: S0006291X09008468
Electromagnetic Biology and Medicine Effect of 900 MHz Radio Frequency Radiation on Beta Amyloid Protein, Protein Carbonyl, and Malondialdehyde in the Brain Informa: 15368378.2011.624654
Electromagnetic Biology and Medicine Do 100- and 500-μT ELF magnetic fields alter beta-amyloid protein, protein carbonyl and malondialdehyde in rat brains? PubMed: 23324065
Indian Journal of Experimental Biology Intranasal administration of insulin lowers amyloid-levels in rat model of diabetes. PubMed: 22279939
European Journal of Pharmacology The γ-secretase inhibitor 2-[(1R)-1-[(4-chlorophenyl)sulfonyl](2,5-difluorophenyl) amino]ethyl-5-fluorobenzenebutanoic acid (BMS-299897) alleviates Aβ1-42 seeding and short-term memory deficits in the Aβ25-35 mouse model of Alzheimer's disease. ScienceDirect: S0014299912009028
Neuropsychopharmacology Blockade of Tau Hyperphosphorylation and Aβ1–42 Generation by the Aminotetrahydrofuran Derivative ANAVEX2-73, a Mixed Muscarinic and σ1 Receptor Agonist, in a Nontransgenic Mouse Model of Alzheimer’s Disease Pubmed: 23493042
世界科技研究与发展(World Sci-tech R & D) 慢病毒介导的CBS基因过表达对原代皮质神经元 Globesci:Source
Neurochemical research Effects of Aluminium on β-Amyloid (1–42) and Secretases (APP-Cleaving Enzymes) in Rat Brain Pubmed:24792732
Neurochem Int Geniposide attenuates insulin-deficiency-induced acceleration of β-amyloidosis in an APP/PS1 transgenic model of Alzheimer&#039;s disease PubMed: 25882165
Journal of Alzheimer’s Parkinsonism & Dementia The Potential Effect of Epigallocatechin-3-Gallate alone or in Combination with Vitamin E and Selenium on Alzheimer's Disease Induced by Aluminum in Rats open-access:
Am J Alzheimers Dis Other Demen. Tong Luo Jiu Nao, a Chinese Medicine Formula, Reduces Inflammatory Stress in a Mouse Model of Alzheimer's Disease Pubmed:26993489
Scientific Reports Norovirus P particle-based active Aβ immunotherapy elicits sufficient immunogenicity and improves cognitive capacity in a mouse model of Alzheimer’s disease articles:srep41041.ris
Journal of Nutritional Biochemistry Mechanisms of DHA-enriched phospholipids in improving cognitive deficits in aged SAMP8 mice with high-fat diet Pubmed:29986309
Journal of Agricultural and Food Chemistry Effects of Astaxanthin and Docosahexaenoic-Acid-Acylated Astaxanthin on Alzheimer's Disease in APP/PS1 Double-Transgenic Mice Pubmed:29695154
Neurotoxicology Amelioration by nitric oxide (NO) mimetics on neurobehavioral and biochemical changes in experimental model of Alzheimer's disease in rats Pubmed:29522778
Oncotarget Deletion of plasma Phospholipid Transfer Protein (PLTP) increases microglial phagocytosis and reduces cerebral amyloid-β deposition in the J20 mouse … Pubmed:29731975
Experimental and Therapeutic Medicine Ellagic acid ameliorates learning and memory impairment in APP/PS1 transgenic mice via inhibition of β‑amyloid production and tau hyperphosphorylation Doi: 10.3892/etm.2018.6860
非编码RNA miR-137 调控tau 蛋白磷酸化和L 型钙离子通道CACNA1C 参与阿尔茨海默病的发生
Neurotoxicity Research Effect of Aluminum-Maltolate on the Content of Aβ Protein and the Expression of ApoER2, VLDLRs, and LRP1 in PC12-ApoE4 Cells Pubmed: 30649678
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