ELISA Kit for Prolactin (PRL)
LTH; Luteotropic Hormone
- Product No.CEA846Ga
- Organism SpeciesChicken (Gallus) Same name, Different species.
- Sample TypeSerum, plasma and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range2.47-200ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.92ng/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 684
For more details, please contact local distributors! US$ 3078
For more details, please contact local distributors! US$ 5814
For more details, please contact local distributors! US$ 47880
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Prolactin (PRL).
No significant cross-reactivity or interference between Prolactin (PRL) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Prolactin (PRL) and the recovery rates were calculated by comparing the measured value to the expected amount of Prolactin (PRL) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prolactin (PRL) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prolactin (PRL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Prolactin (PRL) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Reagent Diluent||1×300µL||Stop Solution||1×6mL|
|TMB Substrate||1×9mL||Instruction manual||1|
|Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|The Journal of Biological Chemistry||In Vivo Evidence for Epidermal Growth Factor Receptor (EGFR)-mediated Release of Prolactin from the Pituitary Gland Jcb: 39297|
|Journal of Clinical Neuroscience||TLR9 expression is associated with prognosis in patients with glioblastoma multiforme ScienceDirect: S0967586811003572|
|Sensors and Actuators B: Chemical||Determination of prolactin hormone in serum and urine using an electrochemical immunosensor based on poly(pyrrolepropionic acid)/carbon nanotubes hybrid modified electrodes Sciencedirect:S0925400514000720|
|Veterinary Word||Effect of extended photoperiod during winter on growth and onset of puberty in Murrah buffalo heifers Pubmed:27051212|
|Journal of Dairy Science||Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calves publication:306322503|
|Catalog No.||Related products for research use of Chicken (Gallus) Organism species||Applications (RESEARCH USE ONLY!)|
|CEA846Ga||ELISA Kit for Prolactin (PRL)||Enzyme-linked immunosorbent assay for Antigen Detection.|