ELISA Kit for Gonadotropin Releasing Hormone (GnRH)
GNRH1; GRH; LNRH; LHRH; LH-RH I; Luliberin; Luteinizing-Hormone Releasing Hormone; Progonadoliberin-1; Gonadoliberin I; Gonadorelin; GnRH-associated peptide 1
- Product No.CEA843Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range12.35-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 4.63pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 684
For more details, please contact local distributors! US$ 3078
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For more details, please contact local distributors! US$ 47880
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Gonadotropin Releasing Hormone (GnRH).
No significant cross-reactivity or interference between Gonadotropin Releasing Hormone (GnRH) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Gonadotropin Releasing Hormone (GnRH) and the recovery rates were calculated by comparing the measured value to the expected amount of Gonadotropin Releasing Hormone (GnRH) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gonadotropin Releasing Hormone (GnRH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gonadotropin Releasing Hormone (GnRH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Gonadotropin Releasing Hormone (GnRH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Mol Pharmacol.||Maternal exposure to dioxin imprints sexual immaturity of the pups through fixing the status of the reduced expression of hypothalamic gonadotropin-releasing … Pubmed:24132183|
|Journal of Sports Science & Medicine||Heavy Resistance Training and Supplementation With the Alleged Testosterone Booster Nmda has No Effect on Body Composition, Muscle Performance, and Serum Hormones Associated With the Hypothalamo-Pituitary-Gonadal Axis in Resistance-Trained Males Pubmed:24570624|
|Environmental Research||Perfluorooctane sulfonate effects on the reproductive axis in adult male rats Pubmed:25171141|
|Toxicology Letters||Possible role of serotonin and neuropeptide Y on the disruption of the reproductive axis activity by perfluorooctane sulfonate Pubmed:25623392|
|Toxicol Sci||Chronic exposure of female mice to an environmental level of perfluorooctane sulfonate suppresses estrogen synthesis through reduced histone H3K14 acetylation of the StAR promoter leading to deficits in follicular development and ovulation PubMed: 26358002|
|Int J Mol Sci.||Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice Pubmed:26771609|
|Oral Presentations||OP0019-HPR Effectiveness of A Progressive Resistance Strength Program on Hand Osteoarthritis: A Randomised Controlled Trial 60.1.abstract|
|Oral Presentations||OP0021-HPR Sleep Disorders in Patients with Rheumatoid Arthritis and Relationship with Disease Activity 60.3.abstract|
|Toxicological Sciences||Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring pubmed:27803384|
|Chronobiology of the immune reponse||OP0020 The Association of Gonadotropin-Releasing Hormone and Cytokines in Rheumatoid Arthritis content:75|
|Environmental Science & Technology||Pyrethroid Insecticide Cypermethrin Accelerates Pubertal Onset in Male Mice via Disrupting Hypothalamic-Pituitary-Gonadal Axis pubmed:28731686|
|Toxicological Sciences||Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons 10.1093:toxsci|
|Catalog No.||Related products for research use of Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA843Ra01||Recombinant Gonadotropin Releasing Hormone (GnRH)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA843Ra01||Polyclonal Antibody to Gonadotropin Releasing Hormone (GnRH)||WB; IHC; ICC; IP.|
|LAA843Ra81||FITC-Linked Polyclonal Antibody to Gonadotropin Releasing Hormone (GnRH)||WB; IHC; ICC; IF.|
|LAA843Ra71||Biotin-Linked Polyclonal Antibody to Gonadotropin Releasing Hormone (GnRH)||WB; IHC; ICC.|
|MAA843Ra21||Monoclonal Antibody to Gonadotropin Releasing Hormone (GnRH)||WB; IHC; ICC; IP.|
|CEA843Ra||ELISA Kit for Gonadotropin Releasing Hormone (GnRH)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSA843Ra11||ELISA Kit DIY Materials for Gonadotropin Releasing Hormone (GnRH)||Main materials for "Do It (ELISA Kit) Yourself".|