ELISA Kit for Von Willebrand Factor (vWF)
F8VWF; VWD; von Willebrand antigen 2
- Product No.CEA833Ca
- Organism SpeciesCanis familiaris; Canine (Dog) Same name, Different species.
- Sample TypePlasma
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range9.88-800ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 3.84ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 720
For more details, please contact local distributors! US$ 3240
For more details, please contact local distributors! US$ 6120
For more details, please contact local distributors! US$ 50400
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Von Willebrand Factor (vWF).
No significant cross-reactivity or interference between Von Willebrand Factor (vWF) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Von Willebrand Factor (vWF) and the recovery rates were calculated by comparing the measured value to the expected amount of Von Willebrand Factor (vWF) in samples.
|Matrix||Recovery range (%)||Average(%)|
|sodium citrate plasma(n=5)||89-97||92|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Von Willebrand Factor (vWF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Von Willebrand Factor (vWF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Von Willebrand Factor (vWF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|sodium citrate plasma(n=5)||78-90%||96-103%||86-101%||85-103%|
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Reagent Diluent||1×300µL||Stop Solution||1×6mL|
|TMB Substrate||1×9mL||Instruction manual||1|
|Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
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|Asian Pac J Trop Med||Blood pH in coronary artery microthrombosis of rats PubMed: 26522304|
|Chemical Research in Toxicology||Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal Instillation PubMed: 25580880|
|Sci Rep.||Differential integrative omic analysis for mechanism insights and biomarker discovery of abnormal Savda syndrome and its unique Munziq prescription Pubmed:27296761|
|American Journal of Reproductive Immunology||Circulating endothelial cells, circulating endothelial progenitor cells, and von Willebrand factor in pregnancies complicated by hypertensive disorders doi:10.1111|
|Shock||Low-Dose Sodium Nitrite Fluid Resuscitation Prevents Lethality From Crush Syndrome by Improving Nitric Oxide Consumption and Preventing Myoglobin Cytotoxicity in Kidney in A Rat Model. pubmed:27941593|
|Regenerative Biomaterials||The directional migration and differentiation of mesenchymal stem cells toward vascular endothelial cells stimulated by biphasic calcium phosphate ceramic 10.1093/rb/rbx028|
|Acta Anaesthesiologica Scandinavica||Early prognostic factors in septic shock cancer patients: a prospective study with a proteomic approach Pubmed:29315472|
|Gynecologic and obstetric investigation||Relationship between the von Willebrand Factor Plasma Concentration and Ultrasonographic Doppler Findings in Pregnancies Complicated by Hypertensive … Pubmed:29621786|
|Biological Trace Element Research||Role of Pigment Epithelium-Derived Factor in Arsenic-Induced Vascular Endothelial Dysfunction in a Rat Model Pubmed: 30392020|
|Experimental and Therapeutic Medicine||Risk stratification and prognostic evaluation of endothelial cell‑specific molecule1, von Willebrand factor, and a disintegrin‑like and metalloprotease with …|
|Catalog No.||Related products for research use of Canis familiaris; Canine (Dog) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA833Ca01||Recombinant Von Willebrand Factor (vWF)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA833Ca01||Polyclonal Antibody to Von Willebrand Factor (vWF)||WB; IHC; ICC; IP.|
|MAA833Ca21||Monoclonal Antibody to Von Willebrand Factor (vWF)||WB; IHC; ICC; IP.|
|CEA833Ca||ELISA Kit for Von Willebrand Factor (vWF)||Enzyme-linked immunosorbent assay for Antigen Detection.|