ELISA Kit for Cholecystokinin (CCK)
- Product No.CEA802Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample TypeSerum, plasma and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range9.88-800pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 4.22pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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For more details, please contact local distributors! US$ 47880
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This assay has high sensitivity and excellent specificity for detection of Cholecystokinin (CCK).
No significant cross-reactivity or interference between Cholecystokinin (CCK) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Cholecystokinin (CCK) and the recovery rates were calculated by comparing the measured value to the expected amount of Cholecystokinin (CCK) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cholecystokinin (CCK) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cholecystokinin (CCK) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cholecystokinin (CCK) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Reagent Diluent||1×300µL||Stop Solution||1×6mL|
|TMB Substrate||1×9mL||Instruction manual||1|
|Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Journal of Obstetrics and Gynaecology Research?||Disturbed release of cholecystokinin in pregnant women with hyperemesis gravidarum Pubmed:25331205|
|World J Gastroenterol||Effects of sphincter of Oddi motility on the formation of cholesterol gallstones Pubmed:27350732|
|Journal of Ethnopharmacology||Luhong formula inhibits myocardial fibrosis in a paracrine manner by activating thegp130/JAK2/STAT3 pathway in cardiomyocytes. pubmed:28115285|
|Cellular physiology and biochemistry||Cholecystokinin Expression in the Development of Postinfarction Heart Failure. pubmed:29130944|
|JOURNAL OF PHARMACEUTICAL SCIENCES||Assessment of the role of cholecystokinin in hyperemesis gravidarum and correlation with its severity :|
|Journal of Dairy Science||Effects of dietary leucine and phenylalanine on pancreas development, enzyme activity, and relative gene expression in milk-fed Holstein dairy calves Pubmed:29477524|
|Food and Nutrition Research||Alignments of endocrine, anthropometric, and metabolic parameters in type 2 diabetes after intervention with an Okinawa-based Nordic diet Pubmed:29599686|
|International Heart Journal||The Relationship of Appetite-Regulating Hormones in the Development of Cardiac Cachexia Pubmed: 30799377|
|Catalog No.||Related products for research use of Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA802Ra01||Recombinant Cholecystokinin (CCK)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA802Ra01||Polyclonal Antibody to Cholecystokinin (CCK)||WB; IHC; ICC; IP.|
|LAA802Ra71||Biotin-Linked Polyclonal Antibody to Cholecystokinin (CCK)||WB; IHC; ICC.|
|MAA802Ra21||Monoclonal Antibody to Cholecystokinin (CCK)||WB; IHC; ICC; IP.|
|CEA802Ra||ELISA Kit for Cholecystokinin (CCK)||Enzyme-linked immunosorbent assay for Antigen Detection.|