ELISA Kit for Cholecystokinin (CCK)

CCK-PZ

Specificity

This assay has high sensitivity and excellent specificity for detection of Cholecystokinin (CCK).
No significant cross-reactivity or interference between Cholecystokinin (CCK) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Cholecystokinin (CCK) and the recovery rates were calculated by comparing the measured value to the expected amount of Cholecystokinin (CCK) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 79-94 83
EDTA plasma(n=5) 79-91 81
heparin plasma(n=5) 85-94 89

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cholecystokinin (CCK) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cholecystokinin (CCK) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cholecystokinin (CCK) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-104% 78-101% 80-102% 94-105%
EDTA plasma(n=5) 83-102% 78-101% 98-105% 95-105%
heparin plasma(n=5) 92-99% 86-101% 85-103% 89-98%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1 Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Reagent Diluent 1×300µL Stop Solution 1×6mL
TMB Substrate 1×9mL Instruction manual 1
Wash Buffer (30 × concentrate) 1×20mL

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

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Magazine Citations
Journal of Obstetrics and Gynaecology Research? Disturbed release of cholecystokinin in pregnant women with hyperemesis gravidarum Pubmed:25331205
World J Gastroenterol Effects of sphincter of Oddi motility on the formation of cholesterol gallstones Pubmed:27350732
Journal of Ethnopharmacology Luhong formula inhibits myocardial fibrosis in a paracrine manner by activating thegp130/JAK2/STAT3 pathway in cardiomyocytes. pubmed:28115285
Cellular physiology and biochemistry Cholecystokinin Expression in the Development of Postinfarction Heart Failure. pubmed:29130944
JOURNAL OF PHARMACEUTICAL SCIENCES Assessment of the role of cholecystokinin in hyperemesis gravidarum and correlation with its severity :
Journal of Dairy Science Effects of dietary leucine and phenylalanine on pancreas development, enzyme activity, and relative gene expression in milk-fed Holstein dairy calves Pubmed:29477524
Food and Nutrition Research Alignments of endocrine, anthropometric, and metabolic parameters in type 2 diabetes after intervention with an Okinawa-based Nordic diet Pubmed:29599686
International Heart Journal The Relationship of Appetite-Regulating Hormones in the Development of Cardiac Cachexia Pubmed: 30799377
Catalog No. Related products for research use of Canis familiaris; Canine (Dog) Organism species Applications (RESEARCH USE ONLY!)
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