ELISA Kit for Insulin (INS)
- Product No.CEA448Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range123.5-10,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 48.5pg/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 648
For more details, please contact local distributors! US$ 2916
For more details, please contact local distributors! US$ 5508
For more details, please contact local distributors! US$ 45360
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Insulin (INS).
No significant cross-reactivity or interference between Insulin (INS) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Insulin (INS) and the recovery rates were calculated by comparing the measured value to the expected amount of Insulin (INS) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin (INS) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin (INS) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Insulin (INS) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Reagent Diluent||1×300µL||Stop Solution||1×6mL|
|TMB Substrate||1×9mL||Instruction manual||1|
|Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Neuroscience Research||Impaired neural stem/progenitor cell proliferation in Streptozotocin-induced and spontaneous diabetic mice PubMed: 20832431|
|International Journal of Nanomedicine||Nicotinamide-functionalized multiwalled carbon nanotubes increase insulin production in pancreatic beta cells via MIF pathway PubMed: PMC3770514|
|Mediators of Inflammation||Metformin Inhibits Expression and Secretion of PEDF in Adipocyte and Hepatocyte via Promoting AMPK Phosphorylation Pubmed: 24288442|
|American Journal of Research Communication||Polo Like Kinase 1 (Plk1) Expression in Visceral Adipose Tissue of Morbidly Obese Non Diabetic Women Usa-journals: Source|
|Nutr Hosp.||Effect of the “protein diet” and bone tissue. Pubmed: 24483972|
|Endocrinology||Rosiglitazone Inhibits Expression and Secretion of PEDF in Adipose Tissue and Liver of Male SD Rats Via a PPAR-γ Independent Mechanism Endocrine: Source|
|CORRELA??ES ENTRE METABóLITOS E HORM?NIOS NO PERíODO DE TRANSI??O DE VACAS DE CORTE DE DIFERENTES GENóTIPOS CRIADAS NO SUL DO BRASIL Cnptia:Source|
|CONCENTRA??ES SéRICAS DE INSULINA NO PóS-PARTO RECENTE DE VACAS DE CORTE DE DIFERENTES GENóTIPOS CRIADAS NO SUL DO BRASIL Alice:Source|
|Evid Based Complement Alternat Med.||Tinospora crispa Ameliorates Insulin Resistance Induced by High Fat Diet in Wistar Rats Pubmed:Pmc4363645|
|Iran J Basic Med Sci||Zataria multiflora increases insulin sensitivity and PPARγ gene expression in high fructose fed insulin resistant rats Pubmed:Pmc4046234|
|Biometals.?||Perinatal low-dose iron treatment influences susceptibility to diet-induced adipogenesis in early-aged male Wistar rats Pubmed:24519243|
|Asian Pac J Cancer Prev.||Effect of NUCKS-1 Overexpression on cytokine profiling in obese women with breast cancer Pubmed:24568505|
|J Ethnopharmacol.?||Antioxidative effects of Korean red ginseng in postmenopausal women: A double-blind randomized controlled trial Pubmed:24814037|
|Obes Surg||Effect of Sleeve Gastrectomy Plus Side-to-Side Jejunoileal Anastomosis for Type 2 Diabetes Control in an Obese Rat Model PubMed: 26202420|
|Evid Based Complement Alternat Med||Tinospora crispa PubMed: 25821506|
|international journal of biological and chemical sciences||Taraxacum officinale Index.Php: Ijbcs|
|Nutrition||Structural and functional abnormalities of hepatic tissues in male Wistar rats fed hyperwhey and super amino anabolic protein Pubmed:27158055|
|European Journal of Pharmacology||Subcutaneous administration of liraglutide ameliorates learning and memory impairment by modulating tau hyperphosphorylation via the glycogen synthase kinase-3尾 pathway in an amyloid 尾 protein induced alzheimer disease mouse model Pubmed:27131827|
|Archives of Physiology and Biochemistry||Improvement of oral contraceptive-induced glucose dysreCavia (Guinea pig )lation and dyslipidemia by valproic acid is independent of circulating corticosterone Pubmed:26855320|
|Archives of Physiology and Biochemistry||Activation of cardiac renin–angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorder Pubmed:26934364|
|Cell and Tissue Research||The dynamic three-dimensional culture of islet-like clusters in decellularized liver scaffolds Pubmed:26796204|
|Adv Biomed Res.||Trigonella foenum-graecum water extract improves insulin sensitivity and stimulates PPAR and γ gene expression in high fructose-fed insulin-resistant rats pmc:PMC4817393|
|Mech Ageing Dev.||Central injection of GALR1 agonist M617 attenuates diabetic rat skeletal muscle insulin resistance through the Akt/AS160/GLUT4 pathway Pubmed:27041232|
|J Bone Miner Res||Fat and Sucrose Intake Induces Obesity‐Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult Rats Pubmed:26175082|
|Scientific Reports||Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus pubmed:27461373|
|International Journal of Medical Research Professionals||Low Circulating Vaspin Level is not associated with Insulinemic Status in Impaired Glucose Tolerant Subjects profile:Muhammad_Saiedullah|
|Journal of Pharmacy and Nutrition Sciences||Metformin Reduces Oxidative Stress Status and Improves Plasma Insulin Level in Streptozotocin-Induced Diabetic Rats pms:index.php|
|EBioMedicine.||Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. pubmed:28336389|
|Journal of Endocrinology and Metabolism||Circulating Betatrophin and Hepatocyte Growth Factor in Type 2 Diabetic Patients: Their Relationship With Disease Prognosis 405|
|American Journal of Translational Research||Subcutaneous liraglutide ameliorates methylglyoxal-induced Alzheimer-like tau pathology and cognitive impairment by modulating tau hyperphosphorylation and glycogen synthase kinase-3β. pubmed:28337257|
|Journal of Molecular and Cellular Cardiology||Irisin improves perivascular adipose tissue dysfunction via regulation of the heme oxygenase-1/adiponectin axis in diet-induced obese mice pubmed:27638193|
|Scientific Reports||The anti-hyperglycemic efficacy of a lipid-lowering drug Daming capsule and the underlyingsignaling mechanisms in a rat model of diabetes mellitus. pubmed:27721485|
|Scientific Reports||Adiponectin protects the rats liver against chronic intermittent hypoxia induced injury throughAMP-activated protein kinase pathway. pubmed:27678302|
|Molecular and Cellular Endocrinology||Baicalin against obesity and insulin resistance through activation of AKT/AS160/GLUT4 pathway pubmed:28359800|
|Diabetologia||Ginsenoside Rg5 attenuates hepatic glucagon response via suppression of succinate-associated HIF-1α induction in HFD-fed mice pubmed:28280902|
|Journal of Diabetes Research||High plasma glucagon levels correlate with waist-to-hip ratio, supra-iliac skinfold thickness, deep subcutaneous abdominal and intra-peritoneal adipose tissue … 2376016.pdf|
|Catalog No.||Related products for research use of Mus musculus (Mouse) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA448Mu02||Recombinant Insulin (INS)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA448Mu01||Recombinant Insulin (INS)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APA448Mu01||Active Insulin (INS)||Cell culture; Activity Assays.|
|PAA448Mu02||Polyclonal Antibody to Insulin (INS)||WB; IHC; ICC; IP.|
|PAA448Mu01||Polyclonal Antibody to Insulin (INS)||WB; IHC; ICC; IP.|
|CEA448Mu||ELISA Kit for Insulin (INS)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSA448Mu11||ELISA Kit DIY Materials for Insulin (INS)||Main materials for "Do It (ELISA Kit) Yourself".|