ELISA Kit for Vasoactive Intestinal Peptide (VIP)
PHM27; Intestinal peptide PHV-42; Peptide histidine valine 42; Peptide histidine methioninamide 27
- Product No.CEA380Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample TypeSerum, plasma and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range6.17-500pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 2.58pg/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Vasoactive Intestinal Peptide (VIP).
No significant cross-reactivity or interference between Vasoactive Intestinal Peptide (VIP) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Vasoactive Intestinal Peptide (VIP) and the recovery rates were calculated by comparing the measured value to the expected amount of Vasoactive Intestinal Peptide (VIP) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vasoactive Intestinal Peptide (VIP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vasoactive Intestinal Peptide (VIP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Vasoactive Intestinal Peptide (VIP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Clinical Genetics||VIP gene variants related to idiopathic pulmonary arterial hypertension in Chinese population Wiley: source|
|Human immunology||Low levels of vasoactive intestinal peptide are associated with Chagas disease cardiomyopathy Pubmed: 23800435|
|Headache: The Journal of Head and Face Pain||CGRP and VIP Levels as Predictors of Efficacy of Onabotulinumtoxin Type A in Chronic Migraine Onlinelibrary: head.12372|
|World J Gastroenterol||Roles of sphincter of Oddi motility and serum vasoactive intestinal peptide, gastrin and cholecystokinin octapeptide NCBI: PMC4000510|
|Cephalalgia?||Increased VIP levels in peripheral blood outside migraine attacks as a potential biomarker of cranial parasympathetic activation in chronic migraine Pubmed:24847167|
|J Huazhong Univ Sci Technolog Med Sci.||Clinical study of electro-acupuncture treatment with different intensities for functional constipation patients Pubmed:25318892|
|PLOS ONE||Aqueous Fraction of Beta vulgaris Ameliorates Hyperglycemia in Diabetic Mice due to Enhanced Glucose Stimulated Insulin Secretion, Mediated by Acetylcholine and GLP-1, and Elevated Glucose Uptake via Increased Membrane Bound GLUT4 Transporters Pubmed:25647228|
|PLOS ONE||Aqueous Fraction of PubMed: 25647228|
|Pharmacol Res||Oral curcumin has anti-arthritic efficacy through somatostatin generation PubMed: 25836921|
|World J Gastroenterol||Effects of sphincter of Oddi motility on the formation of cholesterol gallstones Pubmed:27350732|
|Annals of Allergy, Asthma and Immunology||Effect of acupuncture on house dust mite specific IgE, substance P, and symptoms in persistent allergic rhinitis Pubmed:27156748|
|Cephalalgia.||Relationship between serum levels of VIP, but not of CGRP, and cranial autonomic parasympathetic symptoms: A study in chronic migraine patients Pubmed:27250233|
|Headache||No Change in Interictal PACAP Levels in Peripheral Blood in Women With Chronic Migraine pubmed:27634731|
|Sci Rep.||Using the "target constituent removal combined with bioactivity assay" strategy to investigate the optimum arecoline content in charred areca nut. pubmed:28054652|
|BMC Complementary and Alternative Medicine||-Gingerol, from Zingiber officinale, potentiates GLP-1 mediated glucose-stimulated insulin secretion pathway in pancreatic β-cells and increases RAB8/RAB10-regulated membrane presentation of GLUT4 transporters in skeletal muscle to improve hyperglycemia in Leprdb/db type 2 diabetic mice 10.1186/s12906-017-1903-0|
|Applied Biological Chemistry||Positive enhancement of Lactobacillus fermentum HY01 on intestinal movements of mice having constipation 10.1007/s13765-017-0327-3|
|Catalog No.||Related products for research use of Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA380Ra01||Recombinant Vasoactive Intestinal Peptide (VIP)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA380Ra01||Polyclonal Antibody to Vasoactive Intestinal Peptide (VIP)||WB; IHC; ICC; IP.|
|MAA380Ra21||Monoclonal Antibody to Vasoactive Intestinal Peptide (VIP)||WB; IHC; ICC; IP.|
|CEA380Ra||ELISA Kit for Vasoactive Intestinal Peptide (VIP)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSA380Ra11||ELISA Kit DIY Materials for Vasoactive Intestinal Peptide (VIP)||Main materials for "Do It (ELISA Kit) Yourself".|