ELISA Kit for Anti-Mullerian Hormone (AMH)
MIF; MIH; MIS; Müllerian Inhibiting Factor; Müllerian Inhibiting Hormone; Müllerian Inhibiting Substance
- Product No.CEA228Bo
- Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range61.73-5,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 24.77pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 756
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For more details, please contact local distributors! US$ 52920
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Anti-Mullerian Hormone (AMH).
No significant cross-reactivity or interference between Anti-Mullerian Hormone (AMH) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Anti-Mullerian Hormone (AMH) and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-Mullerian Hormone (AMH) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Mullerian Hormone (AMH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Mullerian Hormone (AMH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-Mullerian Hormone (AMH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Journal of Zhejiang University SCIENCE B||Influence of gonadotropin-releasing hormone agonist on the effect of chemotherapy upon ovarian cancer and the prevention of chemotherapy-induced ovarian damage: an experimental study with nu/nu athymic mice. PubMed: 23125082|
|In Vivo.(Athens,Greece)||Association Between AMH, Oocyte Number and Availability of Embryos for Cryopreservation in IVF Pubmed: 24292596|
|Gynecological Endocrinology||The effects of isotretinoin on the ovarian reserve of females with acne Informahealthcare: Source|
|Fertility and Sterility||Increased expression of anti mullerian hormone and its receptor in endometriosis Ingentaconnect: Source|
|Reproductive BioMedicine Online||Decreased ovarian reserve in female Sprague–Dawley rats induced by isotretinoin (retinoic acid) exposure Rbmojournal: Source|
|Reproductive Biology and Endocrinology||The influence of circulating anti-Müllerian hormone on ovarian responsiveness to ovulation induction with gonadotrophins in women with polycystic ovarian syndrome: a pilot study. Pubmed: 24341292|
|Biomed Res Int.||Oxytocin Improves Follicular Reserve in a Cisplatin-Induced Gonadotoxicity Model in Rats Pubmed:Pmc4053297|
|Fertil Steril.||Increased expression of antimüllerian hormone and its receptor in endometriosis Pubmed:24613539|
|The Journal of Maternal-Fetal & Neonatal Medicine||Maternal serum anti-Müllerian hormone at 11-13 weeks' gestation in the prediction of preeclampsia Pubmed:24953352|
|J Gynecol Oncol.||Granulocyte-colony stimulating factor decreases the extent of ovarian damage caused by cisplatin in an experimental rat model Pubmed:25142624|
|Reproductive BioMedicine Online||The effect of childhood cancer therapy on ovarian reserve and pubertal development Pubmed:25498597|
|Reproductive Sciences||Effects of Resveratrol on Ovarian Morphology, Plasma Anti-Mullerian Hormone, IGF-1 Levels, and Oxidative Stress Parameters in a Rat Model of Polycystic Ovary Syndrome Pubmed:25667201|
|Yonsei Medical Journal||Anti-Proliferative and Apoptotic Activities of Müllerian Inhibiting Substance Combined with Calcitriol in Ovarian Cancer Cell Lines PubMed: 26632380|
|oncology research||Mechanistic Study on Triptorelin Action in Protecting From 5-FU-Induced Ovarian Damage in Rats PubMed: 26629940|
|Reprod Biomed Online||Protective effect of resveratrol against oxidative damage to ovarian reserve in female Sprague–Dawley rats PubMed: 26206282|
|PLoS One||Growth Hormone Ameliorates the Radiotherapy-Induced Ovarian Follicular Loss in Rats: Impact on Oxidative Stress, Apoptosis and IGF-1/IGF-1R Axis PubMed: 26465611|
|Toxicol Lett||Long-term effects of methamphetamine exposure in adolescent mice on the future ovarian reserve in adulthood PubMed: 26657179|
|Journal of Assisted Reproduction and Genetics||Can Coenzyme Q10 supplementation protect the ovarian reserve against oxidative damage? Pubmed:27255570|
|Anticancer Res.||Prognostic Value of Anti-Müllerian Hormone and Inhibin B in Patients with Premenopausal Hormone Receptor-positive Breast Cancer Pubmed:26976997|
|Chemosphere.||Gestational and lactational exposure to bisphenol AF in maternal rats increases testosterone levels in 23-day-old male offspring. pubmed:27567155|
|Theriogenology||Age-related changes in transcriptional abundance and circulating levels of anti-Mullerian hormone and Sertoli cell count in crossbred and Zebu bovine males. pubmed:28043339|
|environmental pollution||Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum. pubmed:27838061|
|Journal of Assisted Reproduction and Genetics||Intrafollicular inflammatory cytokines but not steroid hormone concentrations are increased in naturally matured follicles of women with proven endometriosis. pubmed:28074436|
|Journal Pakistan Medical Association||Serum anti-mullerian hormone: Correlation with the ovarian follicular dynamics in healthy mice pubmed:27654725|
|Advance Medical Associates||Are anti-Müllerian hormone and its receptor polymorphism associated with the hormonal condition of undescended testes? pubmed:27162065|
|Yonsei Med J Volume||Anti-Proliferative and Apoptotic Activities of M? llerian Inhibiting Substance Combined with Calcitriol in Ovarian Cancer Cell Lines. pubmed:26632380|
|Journal of Endocrinological Investigation||The use of serum anti-Mullerian hormone (AMH) levels and antral follicle count (AFC) to predictthe number of oocytes collected and availability of embryos for cryopreservation in IVF. pubmed:27465668|
|Journal of the Pakistan Medical Association||Predictive accuracy of anti mullerian hormone as indicator of ovarian follicle loss in cyclophosphamide treated mice pubmed:28955058|
|International Journal of Reproduction, Contraception, Obstetrics and Gynecology||Can Co-enzyme Q10 improve the chances of conception after the age of 35? 10.18203/2320-1770.ijrcog20172902|
|Animal Production Science||Can serum levels of anti-Müllerian hormone and oestradiol in juvenile gilts be used to predict future reproductive performance? 10.1071/ANv57n12Ab065|
|Reproduction.||Effect of controlled ovarian hyperstimulation on puberty and estrus in mice offspring pubmed:28687593|
|Theriogenology||Relationship among circulating anti-Müllerian hormone, insulin like growth factor 1, cadmium and superovulatory response in dairy cows. pubmed:28708536|
|Journal of ovarian research||Rapamycin Prevents cyclophosphamide-induced Over-activation of Primordial Follicle pool through PI3K/Akt/mTOR Signaling Pathway in vivo. pubmed:28814333|
|Annals of Rheumatic Diseases||FRI0390 Impaired ovarian reserve in patients affected by systemic lupus erythematosus: a case-control study Suppl_2:728.1.abstract|
|Free Radical Biology & Medicine||Peroxiredoxin 2 deficiency accelerates age-related ovarian failure through the reactive oxygen species-mediated JNK pathway in mice Pubmed:29778464|
|Environmental Pollution||Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population Pubmed:29525625|
|Biology of Reproduction||Maternal nutrient restriction impairs young adult offspring ovarian signaling resulting in reproductive dysfunction and follicle loss Pubmed:29351580|
|Endocrine Connections||Post-chemotherapy serum anti-müllerian hormone level predicts ovarian function recovery Pubmed:30012648|
|Reproductive BioMedicine Online||Dienogest suppresses the activation of primordial follicles and preserves the primordial follicle stockpile for fertility in mice Pubmed:29609766|
|Journal of Ovarian Research||Comparison of the influence of three fibroid treatment options: supracervical hysterectomy, ulipristal acetate and uterine artery embolization on ovarian … Pubmed:29859107|
|Animal Reproduction Science||Anti-Müllerian hormone and Oestradiol as markers of future reproductive success in juvenile gilts Pubmed:29859701|
|Stem Cell Research & Therapy||Endometrial mesenchymal stem cells isolated from menstrual blood repaired epirubicin-induced damage to human ovarian granulosa cells by inhibiting the … Pubmed: 30606243|
|Biochemical and Biophysical Research Communications||Involvement of obesity-associated upregulation of chemerin/chemokine-like receptor 1 in oxidative stress and apoptosis in ovaries and granulosa cells Pubmed: 30722991|
|Lupus||Levels of anti-Müllerian hormone in premenopausal women with the antiphospholipid syndrome and its association with the risk of clinical complications Pubmed: 30717622|
|In Vitro Cellular & Developmental Biology - Animal||Transplantation of umbilical cord–derived mesenchymal stem cells on a collagen scaffold improves ovarian function in a premature ovarian failure model of mice Pubmed: 30820812|
|Biomedicine & Pharmacotherapy||Protective properties of glycogen synthase kinase-3 inhibition against doxorubicin-induced oxidative damage to mouse ovarian reserve|
|Reproductive Biology and Endocrinology||Menstrual blood derived mesenchymal stem cells combined with Bushen Tiaochong recipe improved chemotherapy-induced premature ovarian failure in … Pubmed: 31311554|
|Oxidative Medicine and Cellular Longevity||Carvacrol and Thymol Modulate the Cross-Talk between TNF-α and IGF-1 Signaling in Radiotherapy-Induced Ovarian Failure|
|Acta Obstet Gynecol Scand||The impact of uterine artery embolization on ovarian reserve: a systematic review and meta‐analysis Pubmed: 31370100|
|Catalog No.||Related products for research use of Bos taurus; Bovine (Cattle) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA228Bo01||Recombinant Anti-Mullerian Hormone (AMH)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA228Bo01||Polyclonal Antibody to Anti-Mullerian Hormone (AMH)||WB; IHC; ICC; IP.|
|MAA228Bo21||Monoclonal Antibody to Anti-Mullerian Hormone (AMH)||WB; IHC; ICC; IP.|
|CEA228Bo||ELISA Kit for Anti-Mullerian Hormone (AMH)||Enzyme-linked immunosorbent assay for Antigen Detection.|