CLIA Kit for Gonadotropin Releasing Hormone (GnRH)

GNRH1; GRH; LNRH; LHRH; LH-RH I; Luliberin; Luteinizing-Hormone Releasing Hormone; Progonadoliberin-1; Gonadoliberin I; Gonadorelin; GnRH-associated peptide 1

Specificity

This assay has high sensitivity and excellent specificity for detection of Gonadotropin Releasing Hormone (GnRH).
No significant cross-reactivity or interference between Gonadotropin Releasing Hormone (GnRH) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Gonadotropin Releasing Hormone (GnRH) and the recovery rates were calculated by comparing the measured value to the expected amount of Gonadotropin Releasing Hormone (GnRH) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 86-96 91
EDTA plasma(n=5) 98-105 102
heparin plasma(n=5) 82-104 85

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gonadotropin Releasing Hormone (GnRH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gonadotropin Releasing Hormone (GnRH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Gonadotropin Releasing Hormone (GnRH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 96-104% 78-91% 88-103% 95-105%
EDTA plasma(n=5) 94-103% 88-95% 89-103% 85-96%
heparin plasma(n=5) 93-104% 91-101% 88-103% 78-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
7. Read RLU value immediately.

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Toxicological Sciences Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring pubmed:27803384
Chronobiology of the immune reponse OP0020 The Association of Gonadotropin-Releasing Hormone and Cytokines in Rheumatoid Arthritis content:75
Environmental Science & Technology Pyrethroid Insecticide Cypermethrin Accelerates Pubertal Onset in Male Mice via Disrupting Hypothalamic-Pituitary-Gonadal Axis pubmed:28731686
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