CLIA Kit for Insulin (INS)
- Product No.CCA448Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range78.1-20,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 28.3pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 912
For more details, please contact local distributors! US$ 4104
For more details, please contact local distributors! US$ 7752
For more details, please contact local distributors! US$ 63840
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Insulin (INS).
No significant cross-reactivity or interference between Insulin (INS) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Insulin (INS) and the recovery rates were calculated by comparing the measured value to the expected amount of Insulin (INS) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin (INS) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin (INS) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Insulin (INS) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1||Assay Diluent A||1×12mL|
|Detection Reagent B||1×260µL||Assay Diluent B||1×12mL|
|Reagent Diluent||1×300µL||Substrate B||1×2mL|
|Substrate A||1×10mL||Instruction manual||1|
|Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
7. Read RLU value immediately.
|Neuroscience Research||Impaired neural stem/progenitor cell proliferation in Streptozotocin-induced and spontaneous diabetic mice PubMed: 20832431|
|International Journal of Nanomedicine||Nicotinamide-functionalized multiwalled carbon nanotubes increase insulin production in pancreatic beta cells via MIF pathway PubMed: PMC3770514|
|Mediators of Inflammation||Metformin Inhibits Expression and Secretion of PEDF in Adipocyte and Hepatocyte via Promoting AMPK Phosphorylation Pubmed: 24288442|
|American Journal of Research Communication||Polo Like Kinase 1 (Plk1) Expression in Visceral Adipose Tissue of Morbidly Obese Non Diabetic Women Usa-journals: Source|
|Nutr Hosp.||Effect of the “protein diet” and bone tissue. Pubmed: 24483972|
|Endocrinology||Rosiglitazone Inhibits Expression and Secretion of PEDF in Adipose Tissue and Liver of Male SD Rats Via a PPAR-γ Independent Mechanism Endocrine: Source|
|CORRELA??ES ENTRE METABóLITOS E HORM?NIOS NO PERíODO DE TRANSI??O DE VACAS DE CORTE DE DIFERENTES GENóTIPOS CRIADAS NO SUL DO BRASIL Cnptia:Source|
|CONCENTRA??ES SéRICAS DE INSULINA NO PóS-PARTO RECENTE DE VACAS DE CORTE DE DIFERENTES GENóTIPOS CRIADAS NO SUL DO BRASIL Alice:Source|
|Evid Based Complement Alternat Med.||Tinospora crispa Ameliorates Insulin Resistance Induced by High Fat Diet in Wistar Rats Pubmed:Pmc4363645|
|Iran J Basic Med Sci||Zataria multiflora increases insulin sensitivity and PPARγ gene expression in high fructose fed insulin resistant rats Pubmed:Pmc4046234|
|Biometals.?||Perinatal low-dose iron treatment influences susceptibility to diet-induced adipogenesis in early-aged male Wistar rats Pubmed:24519243|
|Asian Pac J Cancer Prev.||Effect of NUCKS-1 Overexpression on cytokine profiling in obese women with breast cancer Pubmed:24568505|
|J Ethnopharmacol.?||Antioxidative effects of Korean red ginseng in postmenopausal women: A double-blind randomized controlled trial Pubmed:24814037|
|Obes Surg||Effect of Sleeve Gastrectomy Plus Side-to-Side Jejunoileal Anastomosis for Type 2 Diabetes Control in an Obese Rat Model PubMed: 26202420|
|Evid Based Complement Alternat Med||Tinospora crispa PubMed: 25821506|
|international journal of biological and chemical sciences||Taraxacum officinale Index.Php: Ijbcs|
|FPO||IDENTIFICATION OF MODULATORS OF BINDING PROPERTIES OF ANTIBODIES REACTIVE WITH A MEMBER OF THE INSULIN RECEPTOR FAMILY Y2015: 0301040.Html|
|Nutrition||Structural and functional abnormalities of hepatic tissues in male Wistar rats fed hyperwhey and super amino anabolic protein Pubmed:27158055|
|European Journal of Pharmacology||Subcutaneous administration of liraglutide ameliorates learning and memory impairment by modulating tau hyperphosphorylation via the glycogen synthase kinase-3尾 pathway in an amyloid 尾 protein induced alzheimer disease mouse model Pubmed:27131827|
|Archives of Physiology and Biochemistry||Improvement of oral contraceptive-induced glucose dysreCavia (Guinea pig )lation and dyslipidemia by valproic acid is independent of circulating corticosterone Pubmed:26855320|
|Archives of Physiology and Biochemistry||Activation of cardiac renin–angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorder Pubmed:26934364|
|Cell and Tissue Research||The dynamic three-dimensional culture of islet-like clusters in decellularized liver scaffolds Pubmed:26796204|
|Adv Biomed Res.||Trigonella foenum-graecum water extract improves insulin sensitivity and stimulates PPAR and γ gene expression in high fructose-fed insulin-resistant rats pmc:PMC4817393|
|Mech Ageing Dev.||Central injection of GALR1 agonist M617 attenuates diabetic rat skeletal muscle insulin resistance through the Akt/AS160/GLUT4 pathway Pubmed:27041232|
|J Bone Miner Res||Fat and Sucrose Intake Induces Obesity‐Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult Rats Pubmed:26175082|
|Scientific Reports||Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus pubmed:27461373|
|International Journal of Medical Research Professionals||Low Circulating Vaspin Level is not associated with Insulinemic Status in Impaired Glucose Tolerant Subjects profile:Muhammad_Saiedullah|
|Journal of Pharmacy and Nutrition Sciences||Metformin Reduces Oxidative Stress Status and Improves Plasma Insulin Level in Streptozotocin-Induced Diabetic Rats pms:index.php|
|33||Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. pubmed:28336389|
|Journal of Endocrinology and Metabolism||Circulating Betatrophin and Hepatocyte Growth Factor in Type 2 Diabetic Patients: Their Relationship With Disease Prognosis 405|
|American Journal of Translational Research||Subcutaneous liraglutide ameliorates methylglyoxal-induced Alzheimer-like tau pathology and cognitive impairment by modulating tau hyperphosphorylation and glycogen synthase kinase-3β. pubmed:28337257|
|Journal of Molecular and Cellular Cardiology||Irisin improves perivascular adipose tissue dysfunction via regulation of the heme oxygenase-1/adiponectin axis in diet-induced obese mice pubmed:27638193|
|Scientific Reports||The anti-hyperglycemic efficacy of a lipid-lowering drug Daming capsule and the underlyingsignaling mechanisms in a rat model of diabetes mellitus. pubmed:27721485|
|Scientific Reports||Adiponectin protects the rats liver against chronic intermittent hypoxia induced injury throughAMP-activated protein kinase pathway. pubmed:27678302|
|Biomedicine & Pharmacotherapy||Vitamin D3 intake as regulator of insulin degrading enzyme and insulin receptor phosphorylationin diabetic rats. pubmed:27930980|
|Eur Neuropsychopharmacol.||Regulation of insulin receptor phosphorylation in the brains of prenatally stressed rats: New insight into the benefits of antidepressant drug treatment. pubmed:28063625|
|Molecular and Cellular Endocrinology||Baicalin against obesity and insulin resistance through activation of AKT/AS160/GLUT4 pathway pubmed:28359800|
|Diabetologia||Ginsenoside Rg5 attenuates hepatic glucagon response via suppression of succinate-associated HIF-1α induction in HFD-fed mice pubmed:28280902|
|Biochimica et Biophysica Acta (BBA)-General Subjects||Modulation of gut microbiota contributes to curcumin-mediated attenuation of hepatic steatosis in rats pubmed:28341485|
|Journal of Diabetes Research||High plasma glucagon levels correlate with waist-to-hip ratio, supra-iliac skinfold thickness, deep subcutaneous abdominal and intra-peritoneal adipose tissue … 2376016.pdf|
|IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES||Berberis integerrima ameliorates insulin resistance in high-fructose-fed insulin-resistant rats pubmed:29147484|
|Journal of Functional Foods||Red paprika (Capsicum annuum L.) and its main carotenoid capsanthin ameliorate impaired lipid metabolism in the liver and adipose tissue of high-fat diet-induced obese mice 10.1016:j.jff.2017.01.044|
|BMJ Open Diab Res Care||High circulating plasma dipeptidyl peptidase- 4 levels in non-obese Asian Indians with type 2 diabetes correlate with fasting insulin and LDL-C levels, triceps skinfolds, total intra-abdominal adipose tissue volume and presence of diabetes: a case–control study 10.1136:bmjdrc-2017-000393|
|Archives of Oral Biology||Chronic high-protein diet induces oxidative stress and alters the salivary gland function in rats pubmed:28926744|
|clinical and experimental pharmacology and physiology||C1q/TNF‐related protein 9 improves the anti‐contractile effects of perivascular adipose tissue via the AMPK‐eNOS pathway in diet‐induced obese mice pubmed:28902432|
|Cellular Physiology and Biochemistry||Irisin Regulates Heme Oxygenase-1/ Adiponectin Axis in Perivascular Adipose Tissue and Improves Endothelial Dysfunction in Diet-Induced Obese Mice pubmed:28595178|
|Peptides||Activiated Galanin receptor 2 attenuates insulin resistance in skeletal muscle of obese mice pubmed:29183756|
|Journal of Diabetes Research||High Plasma Glucagon Levels Correlate with Waist-to-Hip Ratio, Suprailiac Skinfold Thickness, and Deep Subcutaneous Abdominal and Intraperitoneal Adipose Tissue Depots in Nonobese Asian Indian Males with Type 2 Diabetes in North India 10.1155/2017/2376016|
|Mechanisms of Ageing and Development||Central injection of GALR1 agonist M617 attenuates diabetic rat skeletal muscle insulin resistance through the Akt/AS160/GLUT4 pathway pubmed:27041232|
|Iranian Journal of Pharmaceutical Research||Antihyperglycemic Effect of Rosa Damascena is Mediated by PPAR.γ Gene Expression in Animal Model of Insulin Resistance pubmed:29201096|
|Obesity Surgery||Effects of Bariatric Surgery on Change of Brown Adipocyte Tissue and Energy Metabolism in Obese Mice pubmed:28853013|
|Journal of Current Medical Research and Practice||Relationship between serum leptin concentration and insulin resistance syndrome in patients with type 2 diabetes mellitus issn/2357-0121|
|Nanomedicine (Lond)||Nanoformulated natural therapeutics for management of streptozotocin-induced diabetes: potential use of curcumin nanoformulation pubmed:28635562|
|Journal of Inorganic Biochemistry||Synthesis and anti-diabetic activity of new N,N-dimethylphenylenediamine-derivatized nitrilotriacetic acid vanadyl complexes. pubmed:28709620|
|Obesity surgery||The Effects of Duodenojejunal Omega Switch in Combination with High-Fat Diet and Control Diet on Incretins, Body Weight, and Glucose Tolerance in Sprague-Dawley Rats. pubmed:28840471|
|Oman Medical Journal||The Role of Insulin Therapy in Correcting Hepcidin Levels in Patients with Type 2 Diabetes Mellitus. pubmed:28584599|
|International Journal of Molecular Medicine||Insulin restores UCP3 activity and decreases energy surfeit to alleviate lipotoxicity in skeletal muscle pubmed:29039450|
|BMC Complementary and Alternative Medicine||Changes in pancreatic histology, insulin secretion and oxidative status in diabetic rats following treatment with Ficus deltoidea and vitexin pubmed:28576138|
|Nutrients||Nypa fruticans Wurmb. Vinegar's Aqueous Extract Stimulates Insulin Secretion and Exerts Hepatoprotective Effect on STZ-Induced Diabetic Rats pubmed:28832548|
|DARU Journal of Pharmaceutical Sciences||Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway pubmed:29020999|
|Journal of Traditional and Complementary Medicine||Improvement of spatial learning and memory, cortical gyrification patterns and brain oxidative stress markers in diabetic rats treated with Ficus deltoidea leaf extract and vitexin. pubmed:29322009|
|World Journal of Pharmaceutical Sciences||The potential role of sitagliptin as an oral treatment regimen for type I diabetes mellitus ISSN: 2321-3086|
|Journal of Endocrinology||Peripheral insulin resistance in ILK-depleted mice by reduction of GLUT4 expression. pubmed:28490443|
|Catalog No.||Related products for research use of Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
|NPA448Ra01||Native Insulin (INS)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA448Ra01||Recombinant Insulin (INS)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA448Ra01||Polyclonal Antibody to Insulin (INS)||WB; IHC; ICC; IP.|
|MAA448Ra21||Monoclonal Antibody to Insulin (INS)||WB; IHC; ICC; IP.|
|CEA448Ra||ELISA Kit for Insulin (INS)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|CCA448Ra||CLIA Kit for Insulin (INS)||Chemiluminescent immunoassay for Antigen Detection.|