CLIA Kit for Insulin (INS)

Specificity

This assay has high sensitivity and excellent specificity for detection of Insulin (INS).
No significant cross-reactivity or interference between Insulin (INS) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Insulin (INS) and the recovery rates were calculated by comparing the measured value to the expected amount of Insulin (INS) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 88-96 92
EDTA plasma(n=5) 96-105 101
heparin plasma(n=5) 79-97 86

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin (INS) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin (INS) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Insulin (INS) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-94% 92-99% 79-96% 87-102%
EDTA plasma(n=5) 80-105% 88-101% 98-105% 81-101%
heparin plasma(n=5) 82-96% 85-99% 80-99% 87-97%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1 Assay Diluent A 1×12mL
Detection Reagent B 1×260µL Assay Diluent B 1×12mL
Reagent Diluent 1×300µL Substrate B 1×2mL
Substrate A 1×10mL Instruction manual 1
Wash Buffer (30 × concentrate) 1×20mL

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
7. Read RLU value immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Neuroscience Research Impaired neural stem/progenitor cell proliferation in Streptozotocin-induced and spontaneous diabetic mice PubMed: 20832431
International Journal of Nanomedicine Nicotinamide-functionalized multiwalled carbon nanotubes increase insulin production in pancreatic beta cells via MIF pathway PubMed: PMC3770514
Mediators of Inflammation Metformin Inhibits Expression and Secretion of PEDF in Adipocyte and Hepatocyte via Promoting AMPK Phosphorylation Pubmed: 24288442
American Journal of Research Communication Polo Like Kinase 1 (Plk1) Expression in Visceral Adipose Tissue of Morbidly Obese Non Diabetic Women Usa-journals: Source
Nutr Hosp. Effect of the “protein diet” and bone tissue. Pubmed: 24483972
Endocrinology Rosiglitazone Inhibits Expression and Secretion of PEDF in Adipose Tissue and Liver of Male SD Rats Via a PPAR-γ Independent Mechanism Endocrine: Source
CORRELA??ES ENTRE METABóLITOS E HORM?NIOS NO PERíODO DE TRANSI??O DE VACAS DE CORTE DE DIFERENTES GENóTIPOS CRIADAS NO SUL DO BRASIL Cnptia:Source
CONCENTRA??ES SéRICAS DE INSULINA NO PóS-PARTO RECENTE DE VACAS DE CORTE DE DIFERENTES GENóTIPOS CRIADAS NO SUL DO BRASIL Alice:Source
Evid Based Complement Alternat Med. Tinospora crispa Ameliorates Insulin Resistance Induced by High Fat Diet in Wistar Rats Pubmed:Pmc4363645
Iran J Basic Med Sci Zataria multiflora increases insulin sensitivity and PPARγ gene expression in high fructose fed insulin resistant rats Pubmed:Pmc4046234
Biometals.? Perinatal low-dose iron treatment influences susceptibility to diet-induced adipogenesis in early-aged male Wistar rats Pubmed:24519243
Asian Pac J Cancer Prev. Effect of NUCKS-1 Overexpression on cytokine profiling in obese women with breast cancer Pubmed:24568505
J Ethnopharmacol.? Antioxidative effects of Korean red ginseng in postmenopausal women: A double-blind randomized controlled trial Pubmed:24814037
Obes Surg Effect of Sleeve Gastrectomy Plus Side-to-Side Jejunoileal Anastomosis for Type 2 Diabetes Control in an Obese Rat Model PubMed: 26202420
Evid Based Complement Alternat Med Tinospora crispa PubMed: 25821506
international journal of biological and chemical sciences Taraxacum officinale Index.Php: Ijbcs
Nutrition Structural and functional abnormalities of hepatic tissues in male Wistar rats fed hyperwhey and super amino anabolic protein Pubmed:27158055
European Journal of Pharmacology Subcutaneous administration of liraglutide ameliorates learning and memory impairment by modulating tau hyperphosphorylation via the glycogen synthase kinase-3尾 pathway in an amyloid 尾 protein induced alzheimer disease mouse model Pubmed:27131827
Archives of Physiology and Biochemistry Improvement of oral contraceptive-induced glucose dysreCavia (Guinea pig )lation and dyslipidemia by valproic acid is independent of circulating corticosterone Pubmed:26855320
Archives of Physiology and Biochemistry Activation of cardiac renin–angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorder Pubmed:26934364
Cell and Tissue Research The dynamic three-dimensional culture of islet-like clusters in decellularized liver scaffolds Pubmed:26796204
Adv Biomed Res. Trigonella foenum-graecum water extract improves insulin sensitivity and stimulates PPAR and γ gene expression in high fructose-fed insulin-resistant rats pmc:PMC4817393
Mech Ageing Dev.  Central injection of GALR1 agonist M617 attenuates diabetic rat skeletal muscle insulin resistance through the Akt/AS160/GLUT4 pathway Pubmed:27041232
J Bone Miner Res Fat and Sucrose Intake Induces Obesity‐Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult Rats Pubmed:26175082
Scientific Reports Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus pubmed:27461373
International Journal of Medical Research Professionals Low Circulating Vaspin Level is not associated with Insulinemic Status in Impaired Glucose Tolerant Subjects profile:Muhammad_Saiedullah
Journal of Pharmacy and Nutrition Sciences Metformin Reduces Oxidative Stress Status and Improves Plasma Insulin Level in Streptozotocin-Induced Diabetic Rats pms:index.php
EBioMedicine. Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. pubmed:28336389
Journal of Endocrinology and Metabolism Circulating Betatrophin and Hepatocyte Growth Factor in Type 2 Diabetic Patients: Their Relationship With Disease Prognosis 405
American Journal of Translational Research Subcutaneous liraglutide ameliorates methylglyoxal-induced Alzheimer-like tau pathology and cognitive impairment by modulating tau hyperphosphorylation and glycogen synthase kinase-3β. pubmed:28337257
Journal of Molecular and Cellular Cardiology Irisin improves perivascular adipose tissue dysfunction via regulation of the heme oxygenase-1/adiponectin axis in diet-induced obese mice pubmed:27638193
Scientific Reports The anti-hyperglycemic efficacy of a lipid-lowering drug Daming capsule and the underlyingsignaling mechanisms in a rat model of diabetes mellitus. pubmed:27721485
Scientific Reports Adiponectin protects the rats liver against chronic intermittent hypoxia induced injury throughAMP-activated protein kinase pathway. pubmed:27678302
Molecular and Cellular Endocrinology Baicalin against obesity and insulin resistance through activation of AKT/AS160/GLUT4 pathway pubmed:28359800
Diabetologia Ginsenoside Rg5 attenuates hepatic glucagon response via suppression of succinate-associated HIF-1α induction in HFD-fed mice pubmed:28280902
Journal of Diabetes Research High plasma glucagon levels correlate with waist-to-hip ratio, supra-iliac skinfold thickness, deep subcutaneous abdominal and intra-peritoneal adipose tissue … 2376016.pdf
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
NPA448Ra01 Native Insulin (INS) Positive Control; Immunogen; SDS-PAGE; WB.
RPA448Ra01 Recombinant Insulin (INS) Positive Control; Immunogen; SDS-PAGE; WB.
PAA448Ra01 Polyclonal Antibody to Insulin (INS) WB; IHC; ICC; IP.
MAA448Ra21 Monoclonal Antibody to Insulin (INS) WB; IHC; ICC; IP.
CEA448Ra ELISA Kit for Insulin (INS) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA448Ra CLIA Kit for Insulin (INS) Chemiluminescent immunoassay for Antigen Detection.