Active Interleukin 24 (IL24)

C49A; FISP; IL10B; MDA7; Mob-5; ST16; Mda-7; Melanoma Differentiation Association Protein 7; Suppression Of Tumorigenicity 16

ACTIVITY TEST

IL24 (interleukin 24) is a cytokine that belongs to IL10 family. This protein can induce apoptosis selectively in various cancer cells, including ECV304. Thus, inhibition of cell proliferation assay of IL24 was conducted using ECV-304 cells. Briefly, ECV-304 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard 1640 prior to the addition of various concentrations of IL24. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Inhibition of ECV-304 cells proliferation after incubation with IIL24 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with various concentrations of IL24 for 48h. The mean OD value of ECV-304 assessed by CCK-8 was shown in Figure 2. It was obvious that IL24 significantly decreased cell viability of ECV-304 cells.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Experimental & Clinical Cancer Research Oncolytic adenovirus armed with IL-24 Inhibits the growth of breast cancer in vitro and in vivo PubMed: PMC3511263
Bioresources and Bioprocessing Improving the mda-7/IL-24 refolding and purification process using optimized culture conditions based on the structure characteristics of inclusion bodies Springer:Source
Clin Exp Pharmacol Physiol. Inhibition of ALDH2 protects PC12 cells against formaldehyde-induced cytotoxicity: involving the protection of hydrogen sulphide. pubmed:28251688
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC064Hu01 Recombinant Interleukin 24 (IL24) Positive Control; Immunogen; SDS-PAGE; WB.
APC064Hu01 Active Interleukin 24 (IL24) Cell culture; Activity Assays.
PAC064Hu01 Polyclonal Antibody to Interleukin 24 (IL24) WB; IHC; ICC; IP.
LAC064Hu71 Biotin-Linked Polyclonal Antibody to Interleukin 24 (IL24) WB; IHC; ICC.
MAC064Hu22 Monoclonal Antibody to Interleukin 24 (IL24) WB; IHC; ICC; IP.
SEC064Hu ELISA Kit for Interleukin 24 (IL24) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC064Hu CLIA Kit for Interleukin 24 (IL24) Chemiluminescent immunoassay for Antigen Detection.