Active Interleukin 24 (IL24)
C49A; FISP; IL10B; MDA7; Mob-5; ST16; Mda-7; Melanoma Differentiation Association Protein 7; Suppression Of Tumorigenicity 16
- Product No.APC064Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point8.6
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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IL24 (interleukin 24) is a cytokine that belongs to IL10 family. This protein can induce apoptosis selectively in various cancer cells, including ECV304. Thus, inhibition of cell proliferation assay of IL24 was conducted using ECV-304 cells. Briefly, ECV-304 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard 1640 prior to the addition of various concentrations of IL24. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Inhibition of ECV-304 cells proliferation after incubation with IIL24 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with various concentrations of IL24 for 48h. The mean OD value of ECV-304 assessed by CCK-8 was shown in Figure 2. It was obvious that IL24 significantly decreased cell viability of ECV-304 cells.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- BCA Protein Quantification Kit
- Molecular Mass Marker for Protein
- Monoclonal Antibody Customized Service
- Polyclonal Antibody Customized Service
- Protein Activity Test Experiment Service
- Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
- Lentivirus Packaging Experiment Service
- Adenovirus Packaging Experiment Service
- Real Time PCR Experimental Service
|Experimental & Clinical Cancer Research||Oncolytic adenovirus armed with IL-24 Inhibits the growth of breast cancer in vitro and in vivo PubMed: PMC3511263|
|Bioresources and Bioprocessing||Improving the mda-7/IL-24 refolding and purification process using optimized culture conditions based on the structure characteristics of inclusion bodies Springer:Source|
|Clin Exp Pharmacol Physiol.||Inhibition of ALDH2 protects PC12 cells against formaldehyde-induced cytotoxicity: involving the protection of hydrogen sulphide. pubmed:28251688|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPC064Hu01||Recombinant Interleukin 24 (IL24)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APC064Hu01||Active Interleukin 24 (IL24)||Cell culture; Activity Assays.|
|PAC064Hu01||Polyclonal Antibody to Interleukin 24 (IL24)||WB; IHC; ICC; IP.|
|LAC064Hu71||Biotin-Linked Polyclonal Antibody to Interleukin 24 (IL24)||WB; IHC; ICC.|
|MAC064Hu22||Monoclonal Antibody to Interleukin 24 (IL24)||WB; IHC; ICC; IP.|
|SEC064Hu||ELISA Kit for Interleukin 24 (IL24)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCC064Hu||CLIA Kit for Interleukin 24 (IL24)||Chemiluminescent immunoassay for Antigen Detection.|