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Active Interleukin 34 (IL34)

C16orf77

ACTIVITY TEST

Interleukin 34 (IL-34) is a protein belonging to a group of cytokines called interleukins. IL-34 increases growth or survival of immune cells known as monocytes; it elicits its activity by binding the Colony stimulating factor 1 receptor. To test the effect of IL-34 on cell proliferation, THP-1 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with 2% serum standard 1640 which contains various concentrations of recombinant human IL-34. After incubated for 4 days, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 2-4 hours at 37℃. Proliferation of THP-1 cells after incubation with IL-34 for 4 days observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant IL-34 for 4 days. The result was shown in Figure 2. It was obvious that IL-34 significantly increased cell viability of THP-1 cells. The ED50 is 0.54ng/mL. (A) THP-1 cells cultured in 1640, stimulated with 10ng/mL IL-34 for 4 days;
(B) Unstimulated THP-1 cells cultured in 1640 for 4days.
Figure 1. Cell proliferation of THP-1 cells after stimulated with IL-34.

Figure. Cell proliferation of THP-1 cells after stimulated with IL-34.

USAGE

Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine Citations
J Int Med Res. Increased Serum Interleukin-34 in Patients with Coronary Artery Disease PubMed: 23206468
Transplantation IL-34 inhibits acute rejection of rat liver transplantation by inducing Kupffer cell M2 polarization Pubmed:29570162
cyberleninka КЛИНИКО-ЭПИДЕМИОЛОГИЧЕСКАЯ ХАРАКТЕРИСТИКА И ОСОБЕННОСТИ БАЛЛАНСА ЦИТОКИНОВ ПРИ КЛЕЩЕВОМ ЭНЦЕФАЛИТЕ В …
Revista da Associacao Medica Brasileira A comparison of IL-17 and IL-34 concentrations in the cerebrospinal fluid of patients with acute inflammatory demyelinating neuropathy and chronic …
Rev Assoc Med Bras A comparison of IL-17 and IL-34 concentrations in the cerebrospinal fluid of patients with acute inflammatory demyelinating neuropathy and chronic?¡­ 33295414
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APC007Hu61 Active Interleukin 34 (IL34) Cell culture; Activity Assays.
APC007Hu01 Active Interleukin 34 (IL34) Cell culture; Activity Assays.
RPC007Hu02 Recombinant Interleukin 34 (IL34) Positive Control; Immunogen; SDS-PAGE; WB.
EPC007Hu62 Eukaryotic Interleukin 34 (IL34) Positive Control; Immunogen; SDS-PAGE; WB.
RPC007Hu01 Recombinant Interleukin 34 (IL34) Positive Control; Immunogen; SDS-PAGE; WB.
PAC007Hu01 Polyclonal Antibody to Interleukin 34 (IL34) WB; IHC; ICC; IP.
MAC007Hu21 Monoclonal Antibody to Interleukin 34 (IL34) WB; IHC; ICC; IP.
MAC007Hu23 Monoclonal Antibody to Interleukin 34 (IL34) WB; IHC; ICC; IP.
MAC007Hu22 Monoclonal Antibody to Interleukin 34 (IL34) WB; IHC; ICC; IP.
SEC007Hu ELISA Kit for Interleukin 34 (IL34) Enzyme-linked immunosorbent assay for Antigen Detection.
SCC007Hu CLIA Kit for Interleukin 34 (IL34) Chemiluminescent immunoassay for Antigen Detection.
LMC007Hu Multiplex Assay Kit for Interleukin 34 (IL34) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.