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Active S100 Calcium Binding Protein A9 (S100A9)

MRP14; MIF; 60B8AG; CFAG; NIF; CAGB; CGLB; L1AG; P14; Calgranulin B; Migration inhibitory factor-related protein 14; Calprotectin L1H subunit; Leukocyte L1 complex heavy chain

ACTIVITY TEST

S100 calcium-binding protein 9 (S100A9) also known as calgranulin B, is a member of the S100 family of secreted calcium binding proteins containing 2 EF-hand calcium-binding motifs.S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Besides, it has been reported that S100A9 can inhibit Hela cells proliferation. To test the effect of S100A9 on cell apoptosis, Hela cells were seeded into 96-well plates at a density of 4,000 cells/well with 1% serum standard DMEM including various concentrations of recombinant human S100A9. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1h at 37℃. Proliferation of Hela cells after incubation with S100A9 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant human S100A9 for 48h. The result was shown in Figure 2. It was obvious that S100A9 significantly inhibit cell viability of Hela cells. The ED50 is 4.8μg/mL.

Figure 2. Inhibition of Hela cells proliferation after stimulated with S100A9.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
J. Proteome Res. Differential Proteomic Analysis Distinguishes Tissue Repair Biomarker Signatures in Wound Exudates Obtained from Normal Healing and Chronic Wounds Pubmed: source
Biochemical and Biophysical Research Communications High circulating levels of S100A8/A9 complex (calprotectin) in male Japanese with abdominal adiposity and dysregulated expression of S100A8 and S100A9 in adipose tissues of obese mice ScienceDirect: S0006291X1200352X
PLoS One. Proteins with Altered Levels in Plasma from Glioblastoma Patients as Revealed by iTRAQ-Based Quantitative Proteomic Analysis PubMed: 23029420
Mainz Identifizierung neuer potentieller Biomarker für das Kolonkarzinom sowie dessen Vorstufen Info:Source
PLOS ONE Susceptibility to COPD: Differential Proteomic Profiling after Acute Smoking Pubmed:25036363
the faseb journal Soluble receptor for advanced glycation end products inhibits disease progression in autosomal dominant polycystic kidney disease by down-regulating cell proliferation PubMed: 25934702
Sci Rep.  S100A9 and ORM1 serve as predictors of therapeutic response and prognostic factors in advanced extranodal NK/T cell lymphoma patients treated with pegaspargase/gemcitabine pmc:PMC4810364
Journal of Alzheimer's Disease Proteomics Analysis of Blood Serums from Alzheimer's Disease Patients Using iTRAQ LabelingTechnology. pubmed:27911324
International journal of molecular sciences Immune Modulating Topical S100A8/A9 Inhibits Growth of Pseudomonas aeruginosa and Mitigates Biofilm Infection in Chronic Wounds. pubmed:28672877
Gastroenterology Tu1938-Micronutrients Assessment in Patients with Cirrhosis 10.1016:S0016-5085(18)33544-3
Gastroenterology Tu1942-PI3K/AKT-Mediated Upregulation of C7 Inhibits Colorectal Cancer Proliferation and Metastasis 10.1016:S0016-5085(18)33544-5
Journal of Biological Chemistry Signaling between pancreatic β-cells and macrophages via S100 calcium-binding protein A8 exacerbates β-cell apoptosis and islet inflammation Pubmed:29496993
Cancer Management and Research The role of calgranulin B gene on the biological behavior of squamous cervical cancer in vitro and in vivo Pubmed:29497331
European review for medical and pharmacological sciences S100A8/A9 promotes MMP-9 expression in the fibroblasts from cardiac rupture after myocardial infarction by inducing macrophages secreting TNFα. Pubmed:29949169
Estudio de células inmunosupresoras y biomarcadores circulantes en carcinoma renal
Lung Mass Spectrometry Analysis of the Exhaled Breath Condensate and Proposal of Dermcidin and S100A9 as Possible Markers for Lung Cancer Prognosis Pubmed: 31115649
中国医药导报 降钙素原, 超敏C 反应蛋白, 红细胞沉降率及S100A9 对肺结核合并肺部细菌感染的影响
scandinavian journal of clinical & laboratory investigation Serum S100A6, S100A8, S100A9 and S100A11 proteins in colorectal neoplasia: results of a single centre prospective study Pubmed: 31856598
PLoS One Adverse reproductive effects of S100A9 on bovine sperm and early embryonic development in vitro Pubmed: 31945120
Sci Rep Overexpression of S100A9 in tumor stroma contribute to immune evasion of NK/T cell lymphoma and predict poor response rate 34045609
university of copenhagen Adjunctive S100A8/A9 Immunomodulation Hinders Cipro fl oxacin Resistance in
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