Active Myostatin (MSTN)

GDF8; Growth Differentiation Factor 8


Figure. The binding activity of MSTN with FSTL3.
Myostatin (MSTN) also known as growth differentiation factor 8 (GDF-8) a myokine, a protein produced and released by myocytes. It inhibit myogenesis including muscle cell growth and differentiation. Myostatin is a secreted growth differentiation factor that is a member of the TGF beta protein family. Besides, Follistatin Like Protein 3 (FSTL3) has been identified as an interactor of MSTN, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat MSTN and recombinant rat FSTL3. Briefly, MSTN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to FSTL3-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-MSTN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of MSTN and FSTL3 was shown in Figure 1, and this effect was in a dose dependent manner.


Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.


Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.


The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.



Magazine Citations
Clinical Medicine and Diagnostics Anti-Myostatin Reduces Bone Mineral Loss in Ovariectomized Rats Sapub:Source
Nutr Metab (Lond) Short-term, high-fat diet accelerates disuse atrophy and protein degradation in a muscle-specific manner in mice PubMed: 26539241
International Journal of Cancer Tumor inoculation site affects the development of cancer cachexia and muscle wasting PubMed: 26016447
Connect Tissue Res Induction of transient tenogenic phenotype of high density cultured human dermal fibroblasts PubMed: 25748814
PLoS One Higher Plasma Myostatin Levels in Cor Pulmonale Secondary to Chronic Obstructive Pulmonary Disease Pubmed:26998756
Calcif Tissue Int. The Effect of Vibration Treatments Combined with Teriparatide or Strontium Ranelate on Bone Healing and Muscle in Ovariectomized Rats Pubmed:27272029
Archives of medical research Irisin and Myostatin Levels in Patients with Graves' Disease. pubmed:27986127
Calcified Tissue International Effect of Strontium Ranelate on the Muscle and Vertebrae of Ovariectomized Rats pubmed:29242963
Scientific Reports Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis. pubmed:28740214
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPB653Ra01 Recombinant Myostatin (MSTN) Positive Control; Immunogen; SDS-PAGE; WB.
APB653Ra01 Active Myostatin (MSTN) Cell culture; Activity Assays.
CPB653Ra21 OVA Conjugated Myostatin (MSTN) Immunogen; SDS-PAGE; WB.
PAB653Ra08 Polyclonal Antibody to Myostatin (MSTN) WB; IHC; ICC; IP.
PAB653Ra01 Polyclonal Antibody to Myostatin (MSTN) WB; IHC; ICC; IP.
LAB653Ra71 Biotin-Linked Polyclonal Antibody to Myostatin (MSTN) WB; IHC; ICC.
MAB653Ra21 Monoclonal Antibody to Myostatin (MSTN) WB; IHC; ICC; IP.
SEB653Ra ELISA Kit for Myostatin (MSTN) Enzyme-linked immunosorbent assay for Antigen Detection.
CEB653Ra ELISA Kit for Myostatin (MSTN) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB653Ra CLIA Kit for Myostatin (MSTN) Chemiluminescent immunoassay for Antigen Detection.
KSB653Ra11 ELISA Kit DIY Materials for Myostatin (MSTN) Main materials for "Do It (ELISA Kit) Yourself".