Active Myostatin (MSTN)
GDF8; Growth Differentiation Factor 8
- Product No.APB653Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 95%
- Isoelectric Point6.8
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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Figure. The binding activity of MSTN with FSTL3.
Myostatin (MSTN) also known as growth differentiation factor 8 (GDF-8) a myokine, a protein produced and released by myocytes. It inhibit myogenesis including muscle cell growth and differentiation. Myostatin is a secreted growth differentiation factor that is a member of the TGF beta protein family. Besides, Follistatin Like Protein 3 (FSTL3) has been identified as an interactor of MSTN, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat MSTN and recombinant rat FSTL3. Briefly, MSTN were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to FSTL3-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-MSTN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of MSTN and FSTL3 was shown in Figure 1, and this effect was in a dose dependent manner.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- BCA Protein Quantification Kit
- Monoclonal Antibody Customized Service
- Polyclonal Antibody Customized Service
- Protein Activity Test Experiment Service
- Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
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|Clinical Medicine and Diagnostics||Anti-Myostatin Reduces Bone Mineral Loss in Ovariectomized Rats Sapub:Source|
|Nutr Metab (Lond)||Short-term, high-fat diet accelerates disuse atrophy and protein degradation in a muscle-specific manner in mice PubMed: 26539241|
|International Journal of Cancer||Tumor inoculation site affects the development of cancer cachexia and muscle wasting PubMed: 26016447|
|Connect Tissue Res||Induction of transient tenogenic phenotype of high density cultured human dermal fibroblasts PubMed: 25748814|
|PLoS One||Higher Plasma Myostatin Levels in Cor Pulmonale Secondary to Chronic Obstructive Pulmonary Disease Pubmed:26998756|
|Calcif Tissue Int.||The Effect of Vibration Treatments Combined with Teriparatide or Strontium Ranelate on Bone Healing and Muscle in Ovariectomized Rats Pubmed:27272029|
|Archives of medical research||Irisin and Myostatin Levels in Patients with Graves' Disease. pubmed:27986127|
|Calcified Tissue International||Effect of Strontium Ranelate on the Muscle and Vertebrae of Ovariectomized Rats pubmed:29242963|
|Scientific Reports||Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis. pubmed:28740214|
|Catalog No.||Related products for research use of Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
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|SEB653Ra||ELISA Kit for Myostatin (MSTN)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|CEB653Ra||ELISA Kit for Myostatin (MSTN)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCB653Ra||CLIA Kit for Myostatin (MSTN)||Chemiluminescent immunoassay for Antigen Detection.|
|KSB653Ra11||ELISA Kit DIY Materials for Myostatin (MSTN)||Main materials for "Do It (ELISA Kit) Yourself".|