Active Chitinase-3-like Protein 1 (CHI3L1)

GP39; CHI3-L1; YKL40; Chondrex; Chitinase 3-Like 1; Chondrocyte Protein YKL40; 39 kDa synovial protein

ACTIVITY TEST

Figure. Cell proliferation of spleen cells after stimulated with GP39.<br/>Glycoprotein 39, Cartilage (GP39) also known as Chitinase-3-like protein 1 (CHI3L1), YKL-40 is a secreted glycoprotein that is approximately 40kDa in size that in humans is encoded by the CHI3L1 gene. GP39 may play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. To test the effect of GP39 on cell proliferation, rat spleen cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with 2% serum standard 1640 including various concentrations of recombinant rat GP39. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37℃. Proliferation of spleen cells after incubation with GP39 for 96h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant GP39 for 96h. The result was shown in Figure 2. It was obvious that GP39 significantly increased cell viability of spleen cells.(A) Spleen cells cultured in 1640 stimulated with 10ng/ml GP39 for 96h; (B) Unstimulated spleen cells cultured in 1640 for 96h.

Figure. Cell proliferation of spleen cells after stimulated with GP39.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine Citations
Molecular and Cellular Biochemistry Serum YKL-40: a potential biomarker for psoriasis or endothelial dysfunction in psoriasis? Pubmed:25421412
BMC Pulmonary Medicine YKL-40 is correlated with FEV1 and the asthma control test (ACT) in asthmatic patients: influence of treatment Pubmed:25578181
International Journal of Rheumatic Diseases Assessment of pulsed electromagnetic field therapy with Serum YKL‐40 and ultrasonography in patients with knee osteoarthritis PubMed: 25955771
Respiratory Research YKL-40 expression in chronic obstructive pulmonary disease: relation to acute exacerbations and airway remodeling Pubmed:27013031
United States Patent Application Compositions and Methods for Diagnosing Lung Cancer y2016:0077095.html
Chronic obstructive pulmonary disease (COPD)--from biomarkers to clinical phenotypes ISBN:978-951-51-4039-5
EYE YKL-40 is a local marker for inflammation in patients with pseudoexfoliation syndrome Pubmed: 30560917
European Review for Medical and Pharmacological Sciences Astrocytes induce proliferation of oligodendrocyte progenitor cells via connexin 47-mediated activation of Chi3l1 expression Pubmed: 31002152
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
APB463Ra01 Active Chitinase-3-like Protein 1 (CHI3L1) Cell culture; Activity Assays.
RPB463Ra01 Recombinant Chitinase-3-like Protein 1 (CHI3L1) Positive Control; Immunogen; SDS-PAGE; WB.
PAB463Ra01 Polyclonal Antibody to Chitinase-3-like Protein 1 (CHI3L1) WB; IHC; ICC; IP.
MAB463Ra21 Monoclonal Antibody to Chitinase-3-like Protein 1 (CHI3L1) WB; IHC; ICC; IP.
SEB463Ra ELISA Kit for Chitinase-3-like Protein 1 (CHI3L1) Enzyme-linked immunosorbent assay for Antigen Detection.