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Active Granzyme K (GZMK)

GZM-K; TRYP2; PRSS; Serine Protease,Granzyme 3; Tryptase II; Fragmentin-3; Granzyme-3; NK-tryptase-2

ACTIVITY TEST

Granzyme K is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Mouse granzyme K is synthesized as a precursor (263 residues) with a signal peptide (residues 121), a propeptide (residues 22-25) and a mature chain (residues 26-263). The activity of recombinant mouse Granzyme K was measured by its ability to cleaves a thioester substrate Z-Lys-SBzl•HCl. The reaction was performed in 0.05 M Tris, 0.15 M NaCl, 0.01% Triton X-100, pH 8.0 (assay buffer), initiated by addition 50 μL of various concentrations of GZMK (diluted by assay buffer) to 50 µL of 1.2 mM substrate and DTNB mixture. The final well serves as a negative control with no GZMK, replace with 50 μL assay buffer. Incubated at 25℃ for 5min, then read at a wavelength of 405 nm. The specific activity of recombinant mouse Granzyme K is >70 pmol/min/µg.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Immunobiology. Granzyme M and K release in human experimental endotoxemia science:S0171298516300225
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB209Mu01 Recombinant Granzyme K (GZMK) Positive Control; Immunogen; SDS-PAGE; WB.
APB209Mu01 Active Granzyme K (GZMK) Cell culture; Activity Assays.
RPB209Mu02 Recombinant Granzyme K (GZMK) Positive Control; Immunogen; SDS-PAGE; WB.
PAB209Mu01 Polyclonal Antibody to Granzyme K (GZMK) WB; IHC; ICC; IP.
PAB209Mu02 Polyclonal Antibody to Granzyme K (GZMK) WB; IHC; ICC; IP.
MAB209Mu25 Monoclonal Antibody to Granzyme K (GZMK) WB.
MAB209Mu22 Monoclonal Antibody to Granzyme K (GZMK) WB; IHC; ICC; IP.
MAB209Mu23 Monoclonal Antibody to Granzyme K (GZMK) WB; IHC; ICC; IP.
MAB209Mu27 Monoclonal Antibody to Granzyme K (GZMK) WB; IHC; ICC; IP.
MAB209Mu29 Monoclonal Antibody to Granzyme K (GZMK) WB; IHC; ICC; IP.
MAB209Mu24 Monoclonal Antibody to Granzyme K (GZMK) WB; IHC; ICC; IP.
MAB209Mu21 Monoclonal Antibody to Granzyme K (GZMK) WB; IHC; ICC; IP.
SEB209Mu ELISA Kit for Granzyme K (GZMK) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB209Mu Multiplex Assay Kit for Granzyme K (GZMK) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.