Active Lactoferrin (LTF)
HLF2; LF; GIG12; Lactotransferrin; Growth-inhibiting protein 12; Talalactoferrin; Lfcin-H; Kaliocin-1
- Product No.APA780Cp01
- Organism SpeciesCapra hircus; Caprine (Goat) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 97%
- Isoelectric Point8.9
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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Figure. The binding activity of LTF with CLU.
Lactotransferrin (LTF), also known as actoferrin (LF) , is a multifunctional protein of the transferrin family. Lactoferrin belongs to the innate immune system. Apart from its main biological function, namely binding and transport of iron ions, lactoferrin also has antibacterial, antiviral, antiparasitic, catalytic, anti-cancer, and anti-allergic functions and properties. LTF is widely represented in various secretory fluids, such as milk, saliva, tears, and nasal secretions. It also present in secondary granules of PMN and is secreted by some acinar cells. Besides, Clusterin (CLU) has been identified as an interactor of LTF, thus a binding ELISA assay was conducted to detect the interaction of recombinant goat LTF and recombinant goat CLU. Briefly, LTF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100µL were then transferred to CLU-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-LTF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of LTF and CLU was shown in Figure 1, and this effect was in a dose dependent manner.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- BCA Protein Quantification Kit
- Molecular Mass Marker for Protein
- Monoclonal Antibody Customized Service
- Polyclonal Antibody Customized Service
- Protein Activity Test Experiment Service
- Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
- Lentivirus Packaging Experiment Service
- Adenovirus Packaging Experiment Service
- Real Time PCR Experimental Service
|The International Journal of Biochemistry & Cell Biology||Changes of hepatic lactoferrin gene expression in two mouse models of the acute phase reaction ScienceDirect: S1357272511002524|
|BMC Veterinary Research||Changes in composition of colostrum of Egyptian buffaloes and Holstein cows BioMed: 17466148|
|Plos One||p38MAPK, ERK and PI3K Signaling Pathways Are Involved in C5a-Primed Neutrophils for ANCA-Mediated Activation PlosOne: source|
|The Journal of Biological Chemistry||Sphingolipid Pathway Regulates Innate Immune Responses at the Fetomaternal Interface during Pregnancy Pubmed:25505239|
|PLoS One||Control of Methicillin-Resistant Staphylococcus aureus Pneumonia Utilizing TLR2 Agonist Pam3CSK4 Pubmed:26974438|
|Bioscience, Biotechnology, and Biochemistry||Salivary lactoferrin is transferred into the brain via the sublingual route pubmed:28351211|
|Pulmonary Pharmacology & Therapeutics||Effects of fluticasone propionate and budesonide on the expression of immune defense genes in bronchial epithelial cells Pubmed:29627483|
|Microbial Pathogenesis||TLR2 agonist Pam3CSK4 enhances the antibacterial functions of GM-CSF induced neutrophils to methicillin-resistant Staphylococcus aureus|
|Catalog No.||Related products for research use of Capra hircus; Caprine (Goat) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA780Cp01||Recombinant Lactoferrin (LTF)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APA780Cp01||Active Lactoferrin (LTF)||Cell culture; Activity Assays.|
|PAA780Cp01||Polyclonal Antibody to Lactoferrin (LTF)||WB; IHC; ICC; IP.|
|LAA780Cp71||Biotin-Linked Polyclonal Antibody to Lactoferrin (LTF)||WB; IHC; ICC.|