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Active Nesfatin 1 (NES1)

ACTIVITY TEST

Nucb2 (Nucleobindin-2), a calcium-binding protein, is further cleaved into NES1 (Nesfatin-1). NES1 is an anorexigenic peptide and seems to participate in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. GADD45A (Growth arrest and DNA damage-inducible protein GADD45 alpha) has been identified as an interactor of Nucb2. Besides, Growth Arrest And DNA Damage Inducible Protein Beta (GADD45b) has been identified as an interactor of NES1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat NES1 and recombinant rat GADD45b. Briefly, NES1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to GADD45b-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-NES1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of NES1 and GADD45b was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of NES1 with GADD45b.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Bulletin of Clinical Psychopharmacology Plasma Nesfatin-1 Level May Be Associated with Disease Severity in Patients with Panic Disorder Psikofarmakoloji: 2043
Progress in Neuro-Psychopharmacology & Biological Psychiatry High plasma nesfatin-1 level in patients with major depressive disorder PubMed: 21163317
Endocrine Cord blood nesfatin-1 and apelin-36 levels in gestational diabetes mellitus SpringerLink: h978088657555w16
Bulletin of Clinical Psychopharmacology Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsive Disorder Psikofarmakoloji: 2212
Psychoneuroendocrinology. Decreased plasma nesfatin-1 levels in patients with generalized anxiety disorder PubMed: 22595767
Iranian Journal of Health and Physical activity Effects of Aerobic Training, with or without Zizyphus Jujuba Water Extraction, on Fundus Nesfatin-1, ATP, HDL-C, and LDL-C Concentrations in Female Rats Ijvst: Source
European Journal of Obstetrics & Gynecology and Reproductive Biology Decreased serum nesfatin-1 levels in endometriosis Pubmed: 24702902
Bulletin of Clinical Psychopharmacology Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsie Disorder. Psikofarmakoloji: Source
Current Pharmaceutical Design Role of NUCB2/nesfatin-1 as a possible biomarker. Pubmed: 23537082
Regulatory, Integrative and Comparative Physiology A role of nesfatin-1/NucB2 in dehydration-induced anorexia Pubmed:24829503
J Endocrinol Invest. Plasma nesfatin-1 levels are increased in patients with polycystic ovary syndrome Pubmed:24920281
Int J Surg. The potential role of BMI, plasma leptin, nesfatin-1 and ghrelin levels in the early detection of pancreatic necrosis and severe acute pancreatitis: a prospective cohort study. Pubmed:25448651
Hormone molecular biology and clinical investigation The effects of short-term high-intensity interval training vs. moderate-intensity continuous training on plasma levels of nesfatin-1 and inflammatory markers Pubmed:25581765
Turk J Gastroenterol. New hormones to predict the severity of gallstone-induced acute pancreatitis Pubmed:25599787
Respiration Is There a Relationship between Obstructive Sleep Apnea Syndrome Severity and Nesfatin-1? PubMed: 26088934
J Clin Res Pediatr Endocninol Correlation of Brain Neuropeptide (Nesfatin-1 and Orexin-A) Concentrations with Anthropometric and Biochemical Parameters in Malnourished Children PubMed: 26831553
Bosn J Basic Med Sci Nesfatin-1 and Vitamin D levels may be associated with systolic and diastolic blood pressure values and hearth rate in polycystic ovary syndrome PubMed: 26295295
Original Article / Orijinal Makale Relationship and Interaction between Anaerobic Sports Branches and Serum Nesfatin-1 Sayilar: 294
Gynecol Endocrinol Nesfatin-1 levels and metabolic markers in polycystic ovary syndrome PubMed: 26062107
International Journal of Neuroscience Serum nesfatin-1 levels: a potential new biomarker in patients with subarachnoid hemorrhage* Pubmed:26872406
未找到 Comparing the nesfatin-1 levels between individuals with subjective cognitive decline and mild cognitive impairment P.1.e.012.pdf
International Journal of Developmental Serum nesfatin-1 levels: a potential new biomarker in patients with subarachnoid hemorrhage. pubmed:26872406
Current Pharmaceutical Design Metabolic and Cardiovascular Actions of Nesfatin-1: Implications in Health and Disease pubmed:28137218
Arch Endocrinol Metab Serum nesfatin-1 levels are decreased in pregnant women newly diagnosed with gestational diabetes pubmed:28977160
Indian Journal of Psychiatry Serum NUCB2/nesfatin-1 levels in different stages of alcohol dependence: Is there a relationship with craving? pubmed:28529367
Biomedicine & Pharmacotherapy Nesfatin-1 promotes VSMC migration and neointimal hyperplasia by upregulating matrix metalloproteinases and downregulating PPARγ Pubmed:29604590
Biochimica et Biophysica Acta Nesfatin-1 functions as a switch for phenotype transformation and proliferation of VSMCs in hypertensive vascular remodeling Pubmed:29627363
BMC Psychiatry Plasma nesfatin-1 level is associated with severity of depression in Chinese depressive patients Pubmed:29615007
Probiotics and Antimicrobial Proteins Mitra Zarrati, Mahsa Raji Lahiji, Eisa Salehi, Bahareh Yazdani, Elham Razmpoosh, Raheleh Shokouhi Shoormasti & Farzad Shidfar Pubmed: 30232744
An elevated serum level of nesfatin-1 may predict poor outcomes in patients with aneurysmal subarachnoid hemorrhages
clinical neurology and neurosurgery Relationshıp of spontaneous subarachnoid haemorrhage and cerebral aneurysm to serum Visfatin and Nesfatin-1 levels Pubmed: 32311618
Ann Nutr Metab Copeptin and Nesfatin-1 Are Interrelated Biomarkers with Roles in the Pathogenesis of Insulin Resistance in Chinese Children with Obesity Pubmed: 33027789
Endokrynologia Polska Assessing the blood concentration of new adipocytokines in patients with ischaemic stroke 33283259
Increased serum nesfatin-1 levels among adolescents diagnosed with major depressive disorder
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