Active Nesfatin 1 (NES1)
- Product No.APA242Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 97%
- Isoelectric Point5.8
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
For more details, please contact local distributors! US$ 765
For more details, please contact local distributors! US$ 1530
For more details, please contact local distributors! US$ 4590
For more details, please contact local distributors! US$ 11475
For more details, please contact local distributors!
Nucb2 (Nucleobindin-2), a calcium-binding protein, is further cleaved into NES1 (Nesfatin-1). NES1 is an anorexigenic peptide and seems to participate in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. GADD45A (Growth arrest and DNA damage-inducible protein GADD45 alpha) has been identified as an interactor of Nucb2. Besides, Growth Arrest And DNA Damage Inducible Protein Beta (GADD45b) has been identified as an interactor of NES1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat NES1 and recombinant rat GADD45b. Briefly, NES1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to GADD45b-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-NES1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of NES1 and GADD45b was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of NES1 with GADD45b.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
|Bulletin of Clinical Psychopharmacology||Plasma Nesfatin-1 Level May Be Associated with Disease Severity in Patients with Panic Disorder Psikofarmakoloji: 2043|
|Progress in Neuro-Psychopharmacology & Biological Psychiatry||High plasma nesfatin-1 level in patients with major depressive disorder PubMed: 21163317|
|Endocrine||Cord blood nesfatin-1 and apelin-36 levels in gestational diabetes mellitus SpringerLink: h978088657555w16|
|Bulletin of Clinical Psychopharmacology||Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsive Disorder Psikofarmakoloji: 2212|
|Psychoneuroendocrinology.||Decreased plasma nesfatin-1 levels in patients with generalized anxiety disorder PubMed: 22595767|
|Iranian Journal of Health and Physical activity||Effects of Aerobic Training, with or without Zizyphus Jujuba Water Extraction, on Fundus Nesfatin-1, ATP, HDL-C, and LDL-C Concentrations in Female Rats Ijvst: Source|
|European Journal of Obstetrics & Gynecology and Reproductive Biology||Decreased serum nesfatin-1 levels in endometriosis Pubmed: 24702902|
|Bulletin of Clinical Psychopharmacology||Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsie Disorder. Psikofarmakoloji: Source|
|Current Pharmaceutical Design||Role of NUCB2/nesfatin-1 as a possible biomarker. Pubmed: 23537082|
|Regulatory, Integrative and Comparative Physiology||A role of nesfatin-1/NucB2 in dehydration-induced anorexia Pubmed:24829503|
|J Endocrinol Invest.||Plasma nesfatin-1 levels are increased in patients with polycystic ovary syndrome Pubmed:24920281|
|Int J Surg.||The potential role of BMI, plasma leptin, nesfatin-1 and ghrelin levels in the early detection of pancreatic necrosis and severe acute pancreatitis: a prospective cohort study. Pubmed:25448651|
|Hormone molecular biology and clinical investigation||The effects of short-term high-intensity interval training vs. moderate-intensity continuous training on plasma levels of nesfatin-1 and inflammatory markers Pubmed:25581765|
|Turk J Gastroenterol.||New hormones to predict the severity of gallstone-induced acute pancreatitis Pubmed:25599787|
|Respiration||Is There a Relationship between Obstructive Sleep Apnea Syndrome Severity and Nesfatin-1? PubMed: 26088934|
|J Clin Res Pediatr Endocninol||Correlation of Brain Neuropeptide (Nesfatin-1 and Orexin-A) Concentrations with Anthropometric and Biochemical Parameters in Malnourished Children PubMed: 26831553|
|Bosn J Basic Med Sci||Nesfatin-1 and Vitamin D levels may be associated with systolic and diastolic blood pressure values and hearth rate in polycystic ovary syndrome PubMed: 26295295|
|Original Article / Orijinal Makale||Relationship and Interaction between Anaerobic Sports Branches and Serum Nesfatin-1 Sayilar: 294|
|Gynecol Endocrinol||Nesfatin-1 levels and metabolic markers in polycystic ovary syndrome PubMed: 26062107|
|International Journal of Neuroscience||Serum nesfatin-1 levels: a potential new biomarker in patients with subarachnoid hemorrhage* Pubmed:26872406|
|未找到||Comparing the nesfatin-1 levels between individuals with subjective cognitive decline and mild cognitive impairment P.1.e.012.pdf|
|International Journal of Developmental||Serum nesfatin-1 levels: a potential new biomarker in patients with subarachnoid hemorrhage. pubmed:26872406|
|Current Pharmaceutical Design||Metabolic and Cardiovascular Actions of Nesfatin-1: Implications in Health and Disease pubmed:28137218|
|Arch Endocrinol Metab||Serum nesfatin-1 levels are decreased in pregnant women newly diagnosed with gestational diabetes pubmed:28977160|
|Indian Journal of Psychiatry||Serum NUCB2/nesfatin-1 levels in different stages of alcohol dependence: Is there a relationship with craving? pubmed:28529367|
|Biomedicine & Pharmacotherapy||Nesfatin-1 promotes VSMC migration and neointimal hyperplasia by upregulating matrix metalloproteinases and downregulating PPARγ Pubmed:29604590|
|Biochimica et Biophysica Acta||Nesfatin-1 functions as a switch for phenotype transformation and proliferation of VSMCs in hypertensive vascular remodeling Pubmed:29627363|
|BMC Psychiatry||Plasma nesfatin-1 level is associated with severity of depression in Chinese depressive patients Pubmed:29615007|
|Probiotics and Antimicrobial Proteins||Mitra Zarrati, Mahsa Raji Lahiji, Eisa Salehi, Bahareh Yazdani, Elham Razmpoosh, Raheleh Shokouhi Shoormasti & Farzad Shidfar Pubmed: 30232744|
|An elevated serum level of nesfatin-1 may predict poor outcomes in patients with aneurysmal subarachnoid hemorrhages|
|Catalog No.||Related products for research use of Rattus norvegicus (Rat) Organism species||Applications (RESEARCH USE ONLY!)|
|APA242Ra01||Active Nesfatin 1 (NES1)||Cell culture; Activity Assays.|
|RPA242Ra01||Recombinant Nesfatin 1 (NES1)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA242Ra01||Polyclonal Antibody to Nesfatin 1 (NES1)||WB; IHC; ICC; IP.|
|LAA242Ra81||FITC-Linked Polyclonal Antibody to Nesfatin 1 (NES1)||WB; IHC; ICC; IF.|
|LAA242Ra71||Biotin-Linked Polyclonal Antibody to Nesfatin 1 (NES1)||WB; IHC; ICC.|
|SEA242Ra||ELISA Kit for Nesfatin 1 (NES1)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|CEA242Ra||ELISA Kit for Nesfatin 1 (NES1)||Enzyme-linked immunosorbent assay for Antigen Detection.|