Active Superoxide Dismutase 3, Extracellular (SOD3)

SOD-3; EC-SOD; Extracellular Superoxide Dismutase Cu-Zn

ACTIVITY TEST

Extracellular superoxide dismutase [Cu-Zn] is an enzyme that in humans is encoded by the SOD3 gene. This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. Acroding to the report, in a weakly alkaline buffer solution (pH=8.2) with N-tris (hydroxymethyl) amino methane-HCL, pyrogallol can occur autoxidation in the air, then SOD can inhibit this reaction. Thus, we use this way to measure the activity of recombinant human SOD3. The reaction was performed in adding 30μL 50mmol/L pyrogallol to 900μL 50mmol/L Tris-HCl in 1.5ml cuvette (1.0cm light path), rapidly mixing at 25℃,then read at 325nm (zero the spectrophotometer using 50mmol/L Tris-HCl) , reacod the OD value every 30 second for 6 times. Control the pyrogallol autoxidation rate at 0.70 OD/min. After, adding various concentrations of recombinant of SOD3 to 900μL 50mmol/L Tris-HCl, incubated for 20min at 25℃, then adding 30μL 50mmol/L pyrogallol to each tube, rapidly mixing and read at 325nm, record the OD value every 30 second for 6 times. Under these conditions, the enzyme amount of 50% per minute inhibition of pyrogallol autooxidation is defined as a unit.

USAGE

Reconstitute in 10mM PBS (pH7.6) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Current Alzheimer Research Comparison of Extracellular and Intracellular Blood Compartments Highlights Redox Alterations in Alzheimer's and Mild Cognitive Impairment Patients. pubmed:27748187
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Nature Communications SOD3 improves the tumor response to chemotherapy by stabilizing endothelial HIF-2α Pubmed:29422508
BMC Neuroscience Neuroprotective dobutamine treatment upregulates superoxide dismutase 3, anti-oxidant and survival genes and attenuates genes mediating inflammation Pubmed:29523072
Diabetes-Metabolism Research and Reviews Myeloperoxidase, superoxide dismutase‐3, cardiometabolic risk factors, and distal sensorimotor polyneuropathy: The KORA F4/FF4 study Pubmed:29577557
Advances in Medical Sciences Selected elements of extracellular matrix of the skin in diabetes and insulin resistance Pubmed: 31146169
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