Active Nerve Growth Factor (NGF)
NGFB; Beta-NGF; HSAN5; NGF-B; Beta-Nerve Growth Factor
- Product No.APA105Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 97%
- Isoelectric Point10.1
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
For more details, please contact local distributors! US$ 600
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For more details, please contact local distributors! US$ 9000
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Nerve growth factor (NGF) is a neurotrophic factor and neuropeptide primarily involved in the regulation of growth, maintenance, proliferation, and survival of certain target neurons. As reported, when the pheochromocytoma cell line PC12 is exposed to nerve growth factor (NGF), the cells respond over a period of a week by ceasing cell division and extending neurites (Greene and Tischler, 1976). The cells were grown in Ham's F12K containing 5% fetal calf serum and 10% horse serum on polylysine or collagen coated plates. When cells reached log phase growth , fresh medium was added together with 10ng/mL of NGF, then cells were observed by inverted microscope everyday.
Cell division ceasing and differentiation of PC12 cells after incubation with NGF (10ng/mL) for 6 days was shown in Figure1.Control group which received no NGF displayed no neurite outgrowth and cells multiply rapidly.
NGF is also involved in pathways besides those regulating the life cycle of neurons. Besides, Alpha-2-Macroglobulin (a2M) has been identified as an interactor of NGF, thus a binding ELISA assay was conducted to detect the interaction of recombinant human NGF and recombinant human a2M. Briefly, NGF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to a2M-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-NGF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of NGF and a2M was shown in Figure 2, and this effect was in a dose dependent manner.
Figure. The binding activity of NGF with a2M
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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|The Tohoku Journal of Experimental Medicine||Low-intensity aerobic exercise mitigates exercise-induced bronchoconstriction by improving the function of adrenal medullary chromaffin cells in asthmatic rats Pubmed:25212248|
|International Journal of Pharmaceutics||Neurotrophic and antioxidant effects of silymarin comparable to 4-methylcatechol in protection against gentamicin-induced ototoxicity in guinea pigs Pubmed:25445537|
|Yonsei Medical Journal||NGF and HB-EGF: Potential Biomarkers that Reflect the Effects of Fesoterodine in Patients with Overactive Bladder Syndrome Pubmed:25510766|
|Molecular and Cellular Endocrinology||High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 to increase NGF secretion in a rat Schwann cell line RSC96 Pubmed:25614971|
|Mol Cell Endocrinol||High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 PubMed: 25614971|
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|Psychoneuroendocrinology||Chronic mild stress influences nerve growth factor through a matrix metalloproteinase-dependent mechanism Pubmed:26771945|
|Genet Mol Res||Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells Pubmed:27323064|
|The Journal of Maternal-Fetal & Neonatal Medicine||Differential expression of cord blood neurotrophins in gestational diabetes: the impact of fetal growth abnormalities pubmed:28081639|
|IOS Press Content Library||An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … articles:journal-of-alzheimers-disease|
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|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA105Hu01||Recombinant Nerve Growth Factor (NGF)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APA105Hu01||Active Nerve Growth Factor (NGF)||Cell culture; Activity Assays.|
|EPA105Hu61||Eukaryotic Nerve Growth Factor (NGF)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA105Hu01||Polyclonal Antibody to Nerve Growth Factor (NGF)||WB; IHC; ICC; IP.|
|PAA105Hu06||Polyclonal Antibody to Nerve Growth Factor (NGF)||WB; IHC; ICC; IP.|
|LAA105Hu71||Biotin-Linked Polyclonal Antibody to Nerve Growth Factor (NGF)||WB; IHC; ICC.|
|MAA105Hu21||Monoclonal Antibody to Nerve Growth Factor (NGF)||WB; IHC; ICC; IP.|
|MAA105Hu22||Monoclonal Antibody to Nerve Growth Factor (NGF)||WB; IHC; ICC; IP.|
|SEA105Hu||ELISA Kit for Nerve Growth Factor (NGF)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|HEA105Hu||High Sensitive ELISA Kit for Nerve Growth Factor (NGF)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA105Hu||CLIA Kit for Nerve Growth Factor (NGF)||Chemiluminescent immunoassay for Antigen Detection.|
|KSA105Hu01||ELISA Kit DIY Materials for Nerve Growth Factor (NGF)||Main materials for "Do It (ELISA Kit) Yourself".|