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Active Interleukin 11 (IL11)

AGIF; Adipogenesis Inhibitory Factor; Oprelvekin

ACTIVITY TEST

IL11 (Interleukin-11) is a multifunctional cytokine first isolated from bone marrow-derived stromal cells. It stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. Besides, IL11 is reported to induce the proliferation of human T-cells, thus, a proliferation assay was conducted to detect the bioactivity of human recombinant IL11 using Jurkat cells. Briefly, Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 10, 000 cells/well in RPMI-1640 with the addition of various concentrations of IL11. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37℃. Cell proliferation of Jurkat cells after incubation with IL11 for 72h observed by inverted microscope was shown in Figure 1. The CCK-8 data was shown in Figure 2. It was obvious that IL11 significantly promoted cell proliferation of Jurkat cells.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
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Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
EPA057Hu61 Eukaryotic Interleukin 11 (IL11) Positive Control; Immunogen; SDS-PAGE; WB.
APA057Hu01 Active Interleukin 11 (IL11) Cell culture; Activity Assays.
EPA057Hu62 Eukaryotic Interleukin 11 (IL11) Positive Control; Immunogen; SDS-PAGE; WB.
RPA057Hu01 Recombinant Interleukin 11 (IL11) Positive Control; Immunogen; SDS-PAGE; WB.
APA057Hu62 Active Interleukin 11 (IL11) Cell culture; Activity Assays.
APA057Hu61 Active Interleukin 11 (IL11) Cell culture; Activity Assays.
PAA057Hu01 Polyclonal Antibody to Interleukin 11 (IL11) WB; IHC; ICC; IP.
MAA057Hu22 Monoclonal Antibody to Interleukin 11 (IL11) WB; IHC; ICC; IP.
MAA057Hu21 Monoclonal Antibody to Interleukin 11 (IL11) WB; IHC; ICC; IP.
SEA057Hu ELISA Kit for Interleukin 11 (IL11) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA057Hu Multiplex Assay Kit for Interleukin 11 (IL11) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.