ELISA Kit for Anti-Somatostatin Antibody (Anti-SST)

GHIH; SRIF; SMST; GHRIH; GHRIF; Growth Hormone Releasing Inhibiting Hormone; Somatotropin Release-Inhibiting Factor; Somatostatin-14; Somatostatin-28


This assay has high sensitivity and excellent specificity for detection of Anti-Somatostatin Antibody (Anti-SST).
No significant cross-reactivity or interference between Anti-Somatostatin Antibody (Anti-SST) and analogues was observed.


Matrices listed below were spiked with certain level of Anti-Somatostatin Antibody (Anti-SST) and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-Somatostatin Antibody (Anti-SST) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 87-95 91
EDTA plasma(n=5) 87-94 91
heparin plasma(n=5) 92-102 97


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Somatostatin Antibody (Anti-SST) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Somatostatin Antibody (Anti-SST) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-Somatostatin Antibody (Anti-SST) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 92-99% 85-101% 80-105% 98-105%
EDTA plasma(n=5) 87-104% 93-101% 84-91% 82-98%
heparin plasma(n=5) 79-104% 91-105% 79-103% 94-102%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 5 times;
5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
6. Add 50µL Stop Solution. Read at 450nm immediately.



Magazine Citations
JOP. Journal of the Pancreas Pancreatic Somatostatinoma Diagnosed Preoperatively: Report of a Case Pubmed: 24413789
PloS one Circadian Modulation of Anxiety: A Role for Somatostatin in the Amygdala Pubmed: 24376834
Peptides Enhanced expression of neuropeptide S (NPS) receptor in eosinophils from severe asthmatics and subjects with total IgE above 100IU/ml Pubmed:24239856
Journal of Endocrinology Inhibitory effect of somatostatin on insulin secretion is not mediated via the CNS Pubmed:25630331
Pharmacol Res Oral curcumin has anti-arthritic efficacy through somatostatin generation PubMed: 25836921
Acta Neurobiologiae Experimentalis Aberrant changes of somatostatin and neuropeptide Y in brain of a genetic rat model for epilepsy: tremor rat pubmed:27685769
Applied Biological Chemistry Positive enhancement of Lactobacillus fermentum HY01 on intestinal movements of mice having constipation 10.1007/s13765-017-0327-3
Biomed Research International Colonic Transit Time and Gut Peptides in Adult Patients with Slow and Normal Colonic Transit Constipation pubmed:29082242
Molecular Neurobiology Somatostatin Maintains Permeability and Integrity of Blood-Brain Barrier in β-Amyloid Induced Toxicity Pubmed:29700775
Evidence-Based Complementary and Alternative Medicine Home-Based Transcutaneous Neuromodulation Improved Constipation via Modulating Gastrointestinal Hormones and Bile Acids Pubmed:29853946
Pancreatology Relationship between somatostatin and interleukin-6: A cross-sectional study in patients with acute pancreatitis Pubmed: 30279074
Cell Reports Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in Mice Pubmed: 30404008
Science of Food and Agriculture Protective Effect of Silkworm Pupa Oil on Hydrochloric Acid/Ethanol‐Induced Gastric Ulcer Pubmed: 30479041
Catalog No. Related products for research use of Sus scrofa; Porcine (Pig) Organism species Applications (RESEARCH USE ONLY!)
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