Cloud-Clone Active Transforming Growth Factor Beta 1 (TGFb1)

Transforming growth factor beta 1 or TGF-β1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation, and apoptosis.

To test the effect of TGF-β1 on cell apoptosis, A549 cells were seeded into 96-well plates at a density of 5,000 cells/well with 1% serum standard DMEM including various concentrations of recombinant humanTGF-β1. Proliferation of A549 cells after incubation with TGF-β1 for 48h observed by inverted microscope was shown in Figure 1. 

Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant human TGF-β1 for 48h.The result was shown in Figure 2. It was obvious thatTGF-β1 significantly inhibit cell viability of  A549 cells. The ED50 is 6.7μg/ml.

TGF beta 1 has been shown to interact with TGF beta receptor 1, Decorin, LTBP1 and so on. Thus we have conducted a binding ELISA assay to detect the interaction of recombinant human TGF-β1 with recombinant human LTBP1.