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Antibody Pairs Support Pack 1

Instruction manual

First Edition (Revised on April, 2016)

[PRODUCT INFORMATION]

Antibody Pairs Support Pack is intended to use together with Antibody Pairs for ELISA/CLIA kit preparation. This pack provides sufficient reagents for preparation of 10 sandwich ELISA/CLIA plates. All reagents should be used at room temperature and the pack is intended for research purposes only.

[REAGENTS AND MATERIALS PROVIDED]

Reagents

Quantity

Coating Buffer

1 bottle of powder

Blocking Buffer

1 bottle of powder

Standard Diluent 

1 bottle of powder

Detection Antibody Diluent 

1 bottle of powder

Streptavidin-HRP Diluent

1 bottle of powder

Wash Buffer 

2 bottles of powder

Streptavidin-HRP

1 bottle, 1.2ml

Substrate Solution

2 bottles, 48 mL each

Stop Solution

2 bottles, 30 mL each

[APPLICATIONS]

Reagents

Applications

Coating Buffer

Used for coating antibody on the wells of 96-well strip plate. Prepare a working solution by diluting the Coating Buffer with deionized or distilled water before the experiment. 1 bottle of Coating Buffer can be used for preparing 220ml working solution. 

Blocking Buffer

Used for blocking of unspecific binding sites of the plate. Prepare a working solution by diluting the Blocking Buffer with deionized or distilled water before the experiment. 1 bottle of Blocking Buffer can be used for preparing 240ml working solution.

Standard Diluent

Used for dilution of Standard. Prepare a working solution by diluting the Standard Diluent with deionized or distilled water before the experiment. 1 bottle of Standard Diluent can be used for preparing 160ml working solution.

Detection Antibody Diluent

Used for dilution of Detection Antibody. Prepare a working solution by diluting the Detection Antibody Diluent  with deionized or distilled water before the experiment. 1 bottle of Detection Antibody Diluent can be used for preparing 120ml working solution.

Streptavidin-HRP Diluent

Used for dilution of Streptavidin-HRP. Prepare a working solution by diluting the Streptavidin-HRP Diluent  with deionized or distilled water before the experiment. 1 bottle of Streptavidin-HRP Diluent can be used for preparing 120ml working solution.

Wash Buffer

For plate washing. Prepare a working solution by diluting the Wash Buffer with deionized or distilled water before the experiment. 1 bottle of Wash Buffer can be used for preparing 2000ml working solution.

Streptavidin-HRP

Steptavidin-HRP can specifically bind biotinylated antibodies. Prepare a working solution by diluting the Streptavidin-HRP with Streptavidin-HRP Diluent working solution. The dilution ratio was 1:100. 1 bottle can be used for preparing 120ml working solution.

Substrate Solution

Used for chromogenic substrate.

Stop Solution

Used for stopping chromogenic reaction.

NOTE: *All of the working solutions should be clear.

      *If the working solution is prepared, please store at 2-8ºC.


[STORAGE]

Streptavidin-HRP should be stored at -20℃, others are valid for 6 months at 4℃.Store diluted (reconstituted powder) or working solutions at 4 ℃ for up to one week for use in subsequent experiments.

[IMPORTANT NOTES]

Appropriate care should be exercised when using these reagents, including gloves, glasses, lab coats, and good laboratory procedures.