Editing Nucleoside Diphosphate Linked Moiety X-Type Motif 5(NUDT5)

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Summary	Nudix hydrolases, such as NUDT5, eliminate toxic nucleotide derivatives from the cell and regulate the levels of important signaling nucleotides and their metabolites.The deduced 219-amino acid protein has a calculated molecular mass of 24.3 kD. NUDT5 contains a central Nudix motif. Northern blot analysis detected a 1.4-kb transcript in all tissues examined. Minor transcripts of about 5.0 and 1.1 kb were also detected. RNA dot blot analysis revealed NUDT5 expression in all tissues examined. Highest levels were in liver, kidney, pituitary, placenta, and thymus, and lowest levels were in bone marrow, lymph node, and certain areas of brain. Western blot analysis detected NUDT5 at an apparent molecular mass of 33 kD in human nasopharyngeal carcinoma cells, promonocytic cells, and erythrocytes.
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