Editing Nuclear Cap Binding Protein Subunit 1(NCBP1)

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Summary	There is also evidence that the cap can play a role in nuclear events such as splicing, where in vitro assays indicate that it is required for the formation of the spliceosome. Further, it has been suggested that the cap facilitates export of RNAs transcribed by RNA polymerase II from the nucleus to the cytoplasm. Proteins that bind to the cap structure are likely to be involved in these processes. Partially purified protein was sequenced, from which PCR primers were designed and used to isolate a cDNA from a HeLa cell library. The cDNA encodes a predicted 790-amino acid protein. Recombinant epitope-tagged NCBP was exclusively localized to the nucleus and the nuclear localization activity was demonstrated to be restricted to the N-terminal 70 amino acids.
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