Wide-range ELISA Kit for Complement 1q (C1q)
C1-q; Autoantigen Clq
- Product No.WEA747Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample TypeSerum, plasma and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range31.2-2,000ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 13.3ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Wide-range Complement 1q (C1q).
No significant cross-reactivity or interference between Wide-range Complement 1q (C1q) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Wide-range Complement 1q (C1q) and the recovery rates were calculated by comparing the measured value to the expected amount of Wide-range Complement 1q (C1q) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Complement 1q (C1q) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Complement 1q (C1q) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Wide-range Complement 1q (C1q) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Journal of Clinical Immunology||Complement activation contributes to the injury and outcome of kidney in human anti-glomerular basement membrane disease. PubMed: 22941511|
|Fish and Shellfish Immunology||Characterization of complement 1q binding protein of tiger shrimp, Penaeus monodon, and its C1q binding activity Pubmed: 23085472|
|Journal of Neuroimmunology||Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis Pubmed:27235358|
|Archives of Gynecology and Obstetrics||The role of complement components C1q, MBL and C1 inhibitor in pathogenesis of endometriosis Pubmed:29572748|
|Journal of NeuroVirology||Intrathecal complement activation by the classical pathway in tick-borne encephalitis Pubmed: 30850976|
|Catalog No.||Related products for research use of Mus musculus (Mouse) Organism species||Applications (RESEARCH USE ONLY!)|
|WEA747Mu||Wide-range ELISA Kit for Complement 1q (C1q)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SEA747Mu||ELISA Kit for Complement 1q (C1q)||Enzyme-linked immunosorbent assay for Antigen Detection.|