Wide-range ELISA Kit for Corticosterone (Cort)

Corticosrone

Specificity

This assay has high sensitivity and excellent specificity for detection of Wide-range Corticosterone (Cort).
No significant cross-reactivity or interference between Wide-range Corticosterone (Cort) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of Wide-range Corticosterone (Cort) and the recovery rates were calculated by comparing the measured value to the expected amount of Wide-range Corticosterone (Cort) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-99 90
EDTA plasma(n=5) 81-92 88
heparin plasma(n=5) 87-97 93

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Corticosterone (Cort) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Corticosterone (Cort) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Wide-range Corticosterone (Cort) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 84-96% 81-102% 88-95% 87-102%
EDTA plasma(n=5) 90-101% 78-93% 78-97% 93-102%
heparin plasma(n=5) 84-96% 98-105% 81-104% 89-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Respir Res. Intracerebroventricular injection of leukotriene B4 attenuates antigen-induced asthmatic response via BLT1 receptor stimulating HPA-axis in sensitized rats. PubMed: 20403205
Natural Product Sciences Effects of Gypenosides on Acute Stress in Mice Papersearch: Source
Neuropeptides.? Role of adrenomedullin in the cerebrospinal fluid-contacting nucleus in the modulation of immobilization stress Pubmed:25911494
EXPERIMENTAL &amp; CLINICAL CARDIOLOGY Sick Sinus Syndrome May Attribute to the Decrease of ?±-klotho Protein in Mice Com:Source
Journal of Pharmacological Sciences Organ-Specific Activation of the Gastric Branch of the Efferent Vagus Nerve by Ghrelin in Urethane-Anesthetized Rats Pubmed:24366191
Toxicology Letters Regulation of corticosterone secretion is modified by PFOS exposure at different levels of the hypothalamic–pituitary–adrenal axis in adult male rats Pubmed:24440345
Stress. Restraint stress alters immune parameters and induces oxidative stress in the mouse uterus during embryo implantation Pubmed:25238024
Int J Clin Exp Med Diabetes alters the blood glucose response to ketamine in streptozotocin-diabetic rats PubMed: 26379948
Pakistan Veterinary Journal Trivalent Chromium Attenuated Corticosterone Secretion and Actions in Adrenocorticotropic Hormone-Stimulated Rats In_Press: 15-058.Pdf
BMC Ameliorating effects of gypenosides on chronic stress-induced anxiety disorders in mice PubMed: 26370834
Behav Brain Res Rats with differential self-grooming expression in the elevated plus-maze do not differ in anxiety-related behaviors PubMed: 26142783
Archives of Physiology and Biochemistry Improvement of oral contraceptive-induced glucose dysreCavia (Guinea pig )lation and dyslipidemia by valproic acid is independent of circulating corticosterone Pubmed:26855320
Archives of Physiology and Biochemistry Activation of cardiac renin–angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorder Pubmed:26934364
Neuroscience Letters Electroacupuncture reCavia (Guinea pig )late hypothalamic–pituitary–adrenal axis and enhance hippocampal serotonin system in a rat model of depression Pubmed:26773866
Neural Regeneration Research Moxibustion upreCavia (Guinea pig )lates hippocampal progranulin expression. Pubmed:27212922
Environmental Science and Pollution Research Lycopene ameliorates atrazine-induced oxidative damage in adrenal cortex of male rats by activation of the Nrf2/HO-1 pathway Pubmed:27102619
Eur Neuropsychopharmacol Apelin-13 exerts antidepressant-like and recognition memory improving activities in stressed rats Pubmed:26853763
experimental brain research Dopamine D2-like receptors modulate freezing response, but not the activation of HPA axis, during the expression of conditioned fear. pubmed:27766352
brain research bulletin Activation of ATP-sensitive potassium channel by iptakalim normalizes stress-induced HPA axis disorder and depressive behaviour by alleviating inflammation and oxidative stress in mouse hypothalamus. pubmed:28161195
Neurochemical Research  Effects of (-)-Sesamin on Chronic Stress-Induced Anxiety Disorders in Mice pubmed:27995494
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
CPA540Ge11 BSA Conjugated Corticosterone (Cort) Immunogen; SDS-PAGE; WB.
CPA540Ge21 OVA Conjugated Corticosterone (Cort) Immunogen; SDS-PAGE; WB.
PAA540Ge01 Polyclonal Antibody to Corticosterone (Cort) WB; IHC; ICC; IP.
LAA540Ge71 Biotin-Linked Polyclonal Antibody to Corticosterone (Cort) WB; IHC; ICC.
LAA540Ge81 FITC-Linked Polyclonal Antibody to Corticosterone (Cort) WB; IHC; ICC; IF.
MAA540Ge22 Monoclonal Antibody to Corticosterone (Cort) WB; IHC; ICC; IP.
LAA540Ge72 Biotin-Linked Monoclonal Antibody to Corticosterone (Cort) WB; IHC; ICC.
CEA540Ge ELISA Kit for Corticosterone (Cort) Enzyme-linked immunosorbent assay for Antigen Detection.
WEA540Ge Wide-range ELISA Kit for Corticosterone (Cort) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA540Ge CLIA Kit for Corticosterone (Cort) Chemiluminescent immunoassay for Antigen Detection.
KSA540Ge11 ELISA Kit DIY Materials for Corticosterone (Cort) Main materials for "Do It (ELISA Kit) Yourself".