Wide-range ELISA Kit for Corticosterone (Cort)
- Product No.WEA540Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- Sample Typeserum, plasma, urine and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length3h
- Detection Range6.17-500ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 2.73ng/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Wide-range Corticosterone (Cort).
No significant cross-reactivity or interference between Wide-range Corticosterone (Cort) and analogues was observed.
Matrices listed below were spiked with certain level of Wide-range Corticosterone (Cort) and the recovery rates were calculated by comparing the measured value to the expected amount of Wide-range Corticosterone (Cort) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Corticosterone (Cort) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Corticosterone (Cort) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Wide-range Corticosterone (Cort) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Respir Res.||Intracerebroventricular injection of leukotriene B4 attenuates antigen-induced asthmatic response via BLT1 receptor stimulating HPA-axis in sensitized rats. PubMed: 20403205|
|Natural Product Sciences||Effects of Gypenosides on Acute Stress in Mice Papersearch: Source|
|Neuropeptides.?||Role of adrenomedullin in the cerebrospinal fluid-contacting nucleus in the modulation of immobilization stress Pubmed:25911494|
|EXPERIMENTAL & CLINICAL CARDIOLOGY||Sick Sinus Syndrome May Attribute to the Decrease of ?±-klotho Protein in Mice Com:Source|
|Journal of Pharmacological Sciences||Organ-Specific Activation of the Gastric Branch of the Efferent Vagus Nerve by Ghrelin in Urethane-Anesthetized Rats Pubmed:24366191|
|Toxicology Letters||Regulation of corticosterone secretion is modified by PFOS exposure at different levels of the hypothalamic–pituitary–adrenal axis in adult male rats Pubmed:24440345|
|Stress.||Restraint stress alters immune parameters and induces oxidative stress in the mouse uterus during embryo implantation Pubmed:25238024|
|Int J Clin Exp Med||Diabetes alters the blood glucose response to ketamine in streptozotocin-diabetic rats PubMed: 26379948|
|Pakistan Veterinary Journal||Trivalent Chromium Attenuated Corticosterone Secretion and Actions in Adrenocorticotropic Hormone-Stimulated Rats In_Press: 15-058.Pdf|
|BMC||Ameliorating effects of gypenosides on chronic stress-induced anxiety disorders in mice PubMed: 26370834|
|Behav Brain Res||Rats with differential self-grooming expression in the elevated plus-maze do not differ in anxiety-related behaviors PubMed: 26142783|
|Archives of Physiology and Biochemistry||Improvement of oral contraceptive-induced glucose dysreCavia (Guinea pig )lation and dyslipidemia by valproic acid is independent of circulating corticosterone Pubmed:26855320|
|Archives of Physiology and Biochemistry||Activation of cardiac renin–angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorder Pubmed:26934364|
|Neuroscience Letters||Electroacupuncture reCavia (Guinea pig )late hypothalamic–pituitary–adrenal axis and enhance hippocampal serotonin system in a rat model of depression Pubmed:26773866|
|Neural Regeneration Research||Moxibustion upreCavia (Guinea pig )lates hippocampal progranulin expression. Pubmed:27212922|
|Environmental Science and Pollution Research||Lycopene ameliorates atrazine-induced oxidative damage in adrenal cortex of male rats by activation of the Nrf2/HO-1 pathway Pubmed:27102619|
|Eur Neuropsychopharmacol||Apelin-13 exerts antidepressant-like and recognition memory improving activities in stressed rats Pubmed:26853763|
|experimental brain research||Dopamine D2-like receptors modulate freezing response, but not the activation of HPA axis, during the expression of conditioned fear. pubmed:27766352|
|brain research bulletin||Activation of ATP-sensitive potassium channel by iptakalim normalizes stress-induced HPA axis disorder and depressive behaviour by alleviating inflammation and oxidative stress in mouse hypothalamus. pubmed:28161195|
|Neurochemical Research||Effects of (-)-Sesamin on Chronic Stress-Induced Anxiety Disorders in Mice pubmed:27995494|
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