ELISA Kit for Procollagen II C-Terminal Propeptide (PIICP)

P2CP; C-Propeptide Of Type II Procollagen; Procollagen II Carboxy Terminal Propeptide

Specificity

This assay has high sensitivity and excellent specificity for detection of Procollagen II C-Terminal Propeptide (PIICP).
No significant cross-reactivity or interference between Procollagen II C-Terminal Propeptide (PIICP) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Procollagen II C-Terminal Propeptide (PIICP) and the recovery rates were calculated by comparing the measured value to the expected amount of Procollagen II C-Terminal Propeptide (PIICP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 85-101 91
EDTA plasma(n=5) 78-103 98
heparin plasma(n=5) 97-104 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Procollagen II C-Terminal Propeptide (PIICP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Procollagen II C-Terminal Propeptide (PIICP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Procollagen II C-Terminal Propeptide (PIICP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-102% 87-95% 78-104% 95-105%
EDTA plasma(n=5) 88-98% 93-105% 99-105% 83-99%
heparin plasma(n=5) 88-95% 93-102% 97-105% 88-98%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Experimental and Therapeutic Medicine Evaluation of the effect of N-acetyl-glucosamine administration on biomarkers for cartilage metabolism in healthy individuals without symptoms of arthritis: A … 10.3892
Functional Foods in Health & Disease Evaluation of the effect of administering N-acetyl-glucosamine-containing green tea supplement on biomarkers for cartilage metabolism in healthy individuals without symptoms of arthritis: a randomized double-blind placebo-controlled clinical study 309
EXPERIMENTAL AND THERAPEUTIC MEDICINE Effect of N-acetylglucosamine administration on cartilage metabolism and safety in healthy subjects without symptoms of arthritis: A case report pubmed:28413518
Experimental and Therapeutic Medicine Evaluation of the effect of salmon nasal proteoglycan on biomarkers for cartilage metabolism in individuals with knee joint discomfort: A randomized double‑blind placebo‑controlled clinical study etm:14
Experimental and Therapeutic Medicine Evaluation of the efficacy of Ajuga decumbens extract supplement in individuals with knee discomfort associated with physical activity: A randomized, double‑blind, placebo‑controlled study 10.3892/etm.2017.5064
EXPERIMENTAL AND THERAPEUTIC MEDICINE Evaluation of the chondroprotective action of N‑acetylglucosamine in a rat experimental osteoarthritis pubmed:28912864
Functional Foods In Health And Disease Evaluation of the effect of N-acetyl-glucosamine administration on biomarkers for cartilage metabolism in healthy individuals: a randomized double-blind placebo-controlled clinical study view/366
Spine No effects of hyperosmolar culture medium on tissue regeneration by human degenerated nucleus pulposus cells despite upregulation extracellular matrix genes fulltext:2018/03010
Advances in Clinical and Experimental Medicine Fibulin-3 and other cartilage metabolism biomarkers in relationship to calprotectin (MRP8/14) and disease activity in rheumatoid arthritis patients treated with … 68362.pdf
Functional Food Research サケ鼻軟骨由来プロテオグリカン摂取による関節保護効果
Spine No Effects of Hyperosmolar Culture Medium on Tissue Regeneration by Human Degenerated Pubmed: 25856264
Applied Physiology Nutrition and Metabolism Effectiveness of collagen supplementation on pain scores in healthy individuals with self-reported knee pain; A randomized controlled trial Pubmed: 31990581
International Journal of Molecular Sciences Bone Morphogenetic Proteins for Nucleus Pulposus Regeneration Pubmed: 32295299
Biomolecules Concerted Actions by PIICP, CTXII, and TNF-¦Á in Patients with Juvenile Idiopathic Arthritis 33924892
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
CPA964Ra21 OVA Conjugated Procollagen II C-Terminal Propeptide (PIICP) Immunogen; SDS-PAGE; WB.
PAA964Ra08 Polyclonal Antibody to Procollagen II C-Terminal Propeptide (PIICP) WB; IHC; ICC; IP.
SEA964Ra ELISA Kit for Procollagen II C-Terminal Propeptide (PIICP) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA964Ra ELISA Kit for Procollagen II C-Terminal Propeptide (PIICP) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA964Ra Multiplex Assay Kit for Procollagen II C-Terminal Propeptide (PIICP) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA964Ra11 ELISA Kit DIY Materials for Procollagen II C-Terminal Propeptide (PIICP) Main materials for "Do It (ELISA Kit) Yourself".