ELISA Kit for Secretory Immunoglobulin A (sIgA)



This assay has high sensitivity and excellent specificity for detection of Secretory Immunoglobulin A (sIgA).
No significant cross-reactivity or interference between Secretory Immunoglobulin A (sIgA) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Secretory Immunoglobulin A (sIgA) and the recovery rates were calculated by comparing the measured value to the expected amount of Secretory Immunoglobulin A (sIgA) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 84-105 92
EDTA plasma(n=5) 88-96 93
heparin plasma(n=5) 94-103 99


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Secretory Immunoglobulin A (sIgA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Secretory Immunoglobulin A (sIgA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Secretory Immunoglobulin A (sIgA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 81-94% 89-103% 81-90% 82-95%
EDTA plasma(n=5) 98-105% 85-93% 78-93% 91-101%
heparin plasma(n=5) 96-104% 96-104% 79-104% 78-91%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.



Magazine Citations
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PLoS One. Repeated Oronasal Exposure to Lipopolysaccharide Induced Mucosal IgA Responses in Periparturient Dairy Cows Pubmed:25061754
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Front Microbiol Fecal microbiota transplantation and bacterial consortium transplantation have comparable effects on the re-establishment of mucosal barrier function in mice with intestinal dysbiosis PubMed: 26217323
J Hum Lact. Role of Maternal Allergy on Immune Markers in Colostrum and Secretory Immunoglobulin A in Stools of Breastfed Infants PubMed: 26243755
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Biomed Res Int. Salivary Secretory Immunoglobulin (SIgA) and Lysozyme in Malignant Tumor Patients journals:8701423
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Sci Rep.  Long-term use of ceftriaxone sodium induced changes in gut microbiota and immune system. pubmed:28220870
Acta Vet Hung. Imbalance of intestinal immune function in piglets infected by porcine circovirus type 2 during the fetal period. pubmed:28244338
Food & Function Immunomodulatory effects of Hericium erinaceus derived polysaccharides are mediated by intestinal immunology pubmed:28266682
Pathogens and Disease Saccharomyces boulardiiadministration can inhibit the formation of gastric lymphoid follicles induced by Helicobacter suis infection pubmed:28115360
poultry science Effects of dietary threonine supplementation on intestinal barrier function and gut microbiota of laying hens pubmed:28938780
Journal of Functional Foods Novel dietary polysaccharide SIP promotes intestinal secretory immunoglobulin A secretion in mice under chemotherapy 10.1016/j.jff.2017.08.012
International Journal of Pharmacology Evaluation of Susceptibility and Innate Immune Response to Candida albicans in Mice with Sub-health ijp.2018.689.697
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