Packages (Simulation)
![ELISA Kit for Interleukin 17 (IL17)](/upload/static/product/SE.jpg)
Reagent Preparation
![ELISA Kit for Interleukin 17 (IL17)](/upload/static/reagent/TB.jpg)
Image (I)
Image (II)
Certificate
![Certificate](/upload/static/ISO13485.jpg)
![Certificate](/upload/static/ce-1.jpg)
ELISA Kit for Interleukin 17 (IL17)
IL17A; CTLA8; IL-17A; Cytotoxic T-Lymphocyte-Associated Protein 8
- Product No.SEA063Po
- Organism SpeciesSus scrofa; Porcine (Pig) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range15.6-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 5.9pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 529
For more details, please contact local distributors! US$ 756 US$ 3402 US$ 6426 US$ 52920
Specificity
This assay has high sensitivity and excellent specificity for detection of Interleukin 17 (IL17).
No significant cross-reactivity or interference between Interleukin 17 (IL17) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Interleukin 17 (IL17) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 17 (IL17) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 85-96 | 91 |
EDTA plasma(n=5) | 85-93 | 89 |
heparin plasma(n=5) | 79-94 | 84 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 17 (IL17) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 17 (IL17) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 17 (IL17) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 95-102% | 81-101% | 84-99% | 78-90% |
EDTA plasma(n=5) | 89-98% | 81-96% | 90-101% | 78-99% |
heparin plasma(n=5) | 89-96% | 78-89% | 78-95% | 81-94% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
GIVEAWAYS
INCREMENT SERVICES
-
Single-component Reagents of Assay Kit
-
Lysis Buffer Specific for ELISA / CLIA
-
Quality Control of Kit
-
ELISA Kit Customized Service
-
Disease Model Customized Service
-
Serums Customized Service
-
TGFB1 Activation Reagent
-
Real Time PCR Experimental Service
-
Streptavidin
-
Fast blue Protein Stain solution
-
Single-component Reagents of FLIA Kit
-
Streptavidin-Agarose Beads
Magazine | Citations |
PLOS ONE | Impaired Autoimmune T Helper 17 Cell Responses Following DNA Vaccination against Rat Experimental Autoimmune Encephalomyelitis PubMed: PMC2577303 |
Cellular Immunology | Atorvastatin ameliorates experimental autoimmune neuritis by decreased Th1/Th17 cytokines and up-regulated T regulatory cells ScienceDirect: S0008874911002164 |
The Journal of Urology | Improvement by Phytotherapeutic Agent of Detrusor Overactivity, Down-Regulation of Pharmacological Receptors and Urinary Cytokines in Rats with Cyclophosphamide Induced Cystitis PubMed: S0022534712049087 |
Critical Care Medicine | Systemic release of cytokines and heat shock proteins in porcine models of polytrauma and hemorrhage PubMed: PMC3383056 |
Lower Urinary Tract Symptoms | Effects of Saw Palmetto Extract on Urodynamic Parameters, Bladder Muscarinic and Purinergic Receptors and Urinary Cytokines in Rats with Cyclophosphamide-Induced Cystitis Onlinelibrary: luts.12015 |
Shock | Expression of Hemopexin in Acute Rejection of Rat Liver Allograft Identified by Serum Proteomic Analysis. Lww: Source |
J Vet Med Sci. | CD4+ T Cell Cytokine Gene and Protein Expression in Duodenal Mucosa of Dogs with Inflammatory Bowel Disease Pubmed:24270804 |
Journal of Dairy Science | Increased blood-circulating interferon-γ, interleukin-17, and osteopontin levels in bovine paratuberculosis Pubmed:24679935 |
Inflammation.? | Pemetrexed Ameliorates Experimental Arthritis in Rats Pubmed:25129058 |
Inflammation | Lapatinib Ameliorates Experimental Arthritis in Rats Pubmed:25283886 |
Molecular and Cellular Biochemistry | Beta-arrestin-2 negatively modulates inflammation response in mouse chondrocytes induced by 4-mer hyaluronan oligosaccharide Pubmed:25318610 |
Molecular Medicine Reports | Function of interleukin?17 and ?35 in the blood of patients with hepatitis B?related liver cirrhosis Pubmed:25323532 |
Genet Mol Res | Protective effect and mechanism of probucol in the treatment of spinal cord injury PubMed: 26214485 |
veterinary research forum | Evaluation of proliferation and cytokines production by mitogen-stimulated bovine peripheral blood mononuclear cells Article_14936_6.Html: |
Tumor Biology | Hypoxia-induced secretion of IL-20 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma PubMed: 26695151 |
Clinical & Experimental Immunology | MicroRNA‐155 may be involved in the pathogenesis of atopic dermatitis by modulating the differentiation and function of T helper type 17 (Th18) cells PubMed: 25761610 |
J Reprod Immunol | The immunomodulating effect of seminal plasma on T cells PubMed: 25799173 |
Biomarkers | Interleukin-17 levels correlate with poor prognosis and vascular endothelial growth factor concentration in the serum of patients with non-small cell lung cancer PubMed: 26301881 |
Molecular Medicine Reports | Tanshinone IIA attenuates experimental autoimmune encephalomyelitis in rats Pubmed:27357729 |
SpringerPlus | Ethyl pyruvate attenuated coxsackievirus B3-induced acute viral myocarditis by suppression ofHMGB1/RAGE/NF-ΚB pathway. Pubmed:27026909 |
International Immunopharmacology | Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery Pubmed:26802602 |
Journal of Agricultural Biotechnology | Gln Attenuates LPS-induced Inflammatory Injury of Bos taurus Testis Sustentacular Cells 10610 |
Veterinary Research Communications | Immunological and pathological investigations in equine experimental uveitis pubmed:27344152 |
Journal of Neuroinflammation | Th17-skewed immune response and cluster of differentiation 40 ligand expression in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis. pubmed:28114998 |
American Journal of Translational Research | Anti-inflammatory effects of Boletus edulis polysaccharide on asthma pathology pubmed:27830033 |
Springerplus | Ethyl pyruvate attenuated coxsackievirus B3-induced acute viral myocarditis by suppression of HMGB1/RAGE/NF-ΚB pathway pubmed:27026909 |
Journal of Virology | Characterization of Simian Immunodeficiency Virus Variants Anatomically Compartmentalized inPlasma and Milk in Chronically Infected African Green Monkeys. pubmed: |
J Nephropathol | Relationship between IL-17 serum level and ambulatory blood pressure in women with polycystic ovary syndrome pubmed:28042549 |
Journal of Nephropathology | Relationship between IL-17 and ambulatory blood pressure in polycystic ovary syndrome 8b168a09f5832df1b5c2f48631612458b2d8.pdf |
Basic Clin Pharmacol Toxicol. | Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4+ T Helper 17 (Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling. pubmed:28032440 |
Gut Pathog. | Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in mice pubmed:28250847 |
Procalcitonin und proinflammatorische Zytokine in der bronchoalveolären Lavage beim lungenkranken Pferd unter inhalativer Glukokortikoidtherapie FUDISS_thesis_000000103830 | |
Scientific Report | Anti-asthmatic efect of pitavastatin through aerosol inhalation is associated with CD4+ CD25+ Foxp3+ T cells in an asthma mouse model pubmed:28729731 |
Basic & Clinical Pharmacology & Toxicology | Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4 + T Helper 17(Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling doi:10.1111 |
Academic Journal | Antiinflammatory and antioxidant effects of gemcitabine in collagen-induced arthritis model pubmed:28618762 |
International Journal of Clinical and Experimental Pathology | Low-density neutrophils in severe fever with thrombocytopenia syndrome (SFTS) display decreased function to phagocytose SFTS virus and enhanced capacity to synthesize cytokines ISSN:1936-2625/IJCEP0045334 |
Asian Pacific Society of Respirology | Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in mice pubmed:28122397 |
MYOPAIN | Increased Levels of IL-17, IL-23, MIP-1α, MCP-1 and Global Leukocytes in Fibromyalgia Patients 10.1080/24708593.2017.1357664 |
Molecular Medicine Reports | Lower level of IL‑35 and its reduced inhibition in Th17 cells in patients with bone marrow mononuclear cells Coombs test‑positive hemocytopenia. pubmed:29257310 |
Molecular Medicine Reports | Interleukin-17 antagonist attenuates lung inflammation through inhibition of the ERK1/2 and NF-κB pathway in LPS-induced acute lung injury 10.3892/mmr.2017.6837 |
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY | Dexamethasone reduces serum level of IL-17 in Bleomycin-A5-induced rats model of pulmonary fibrosis pubmed:28608724 |
International Immunopharmacology | Therapeutic effect of bone marrow mesenchymal stem cells pretreated with acetylsalicylic acid on experimental periodontitis in rats pubmed:29195233 |
Biomedical Research | Associations among interleukin-17, interferon-gamma, and acute coronary syndrome ISSN 0970-938X |
Cellular Physiology and Biochemistry | Activated Α7nachr Improves Postoperative Cognitive Dysfunction and Intestinal Injury Induced by Cardiopulmonary Bypass in Rats: Inhibition of the Proinflammatory … Pubmed:29672286 |
Frontiers in Pharmacology | Traditional Herbal Medicine-Derived Sulforaphene LFS-01 Reverses Colitis in Mice by Selectively Altering the Gut Microbiota and Promoting Intestinal … Pubmed:29375374 |
Bioscience Reports | Decreased miR-128 expression alleviated the progression of Rheumatoid arthritis by up-regulating the expression of TNFAIP3 Pubmed:29853534 |
Medical Science Monitor | Involvement of T-Helper 9 Activation in a Mouse Model of Allergic Rhinitis Pubmed:29982264 |
Journal of International Medical Research | Changes in Th1/Th2-producing cytokines during acute exacerbation chronic obstructive pulmonary disease Pubmed:29950127 |
Canadian Journal of Physiology and Pharmacology | Inhibition of microRNA-155 attenuates concanavalin A-induced autoimmune hepatitis by regulating Treg/Th17 cell differentiation Pubmed: 30290123 |
BMC Microbiology | Nystatin enhances the immune response against Candida albicans and protects the ultrastructure of the vaginal epithelium in a rat model of vulvovaginal … Pubmed: 30359236 |
Biology of Reproduction | Recombinant adiponectin alleviates abortion in mice by regulating Th17/Treg imbalance via p38MAPK-STAT5 pathway Pubmed: 30496353 |
BMC MICROBIOLOGY | Enhanced antifungal activity of bovine lactoferrin-producing probiotic Lactobacillus casei in the murine model of vulvovaginal candidiasis Pubmed: 30621597 |
CNS Neuroscience & Therapeutics | Negative regulation of glial Tim‐3 inhibits the secretion of inflammatory factors and modulates microglia to antiinflammatory phenotype after experimental intracerebral?… Pubmed: 30677253 |
OPEN ACCESS MACEDONIAN JOURNAL OF MEDICAL SCIENCES | Seluang Fish (Rasbora Spp.) Oil Decreases Inflammatory Cytokines Via Increasing Vitamin D Level in Systemic Lupus Erythematosus |
Frontiers in Microbiology | Local probiotic Lactobacillus crispatus and Lactobacillus delbrueckii exhibit strong antifungal effects against vulvovaginal candidiasis in a rat model |
中国免疫学杂志 | TRAF-6 调控TGF-β1-Smad2/Smad3 信号通路在Graves 病免疫发病机制中的作用 |
Microbial Pathogenesis | Therapeutic effectiveness of type I interferon in vulvovaginal candidiasis Pubmed: 31158491 |
Inflammation | Protein Kinase C Theta Inhibition Attenuates Lipopolysaccharide-Induced Acute Lung Injury through Notch Signaling Pathway via Suppressing Th17 Cell Response … Pubmed: 31297750 |
international immunopharmacology | Adenovirus-mediated down-regulation of miR-21-5p alleviates experimental autoimmune uveoretinitis in mice Pubmed: 31228813 |
Untersuchung einer Th17-vermittelten Immunantwort bei der kaninen idiopathischen Epilepsie | |
Free Radical Biology and Medicine | Vitamin D protects against oxidative stress, inflammation and hepatorenal damage induced by acute paracetamol toxicity in rat Pubmed: 31255668 |
BioMed Research International | Indoleamine 2, 3-Dioxygenase (IDO) Regulates Th17/Treg Immunity in Experimental IgA Nephropathy |
Animals | Anti-Inflammatory State in Arabian Horses Introduced to the Endurance Training Pubmed: 31462005 |
World Journal of Pediatrics | Regulation of JAK/STAT signal pathway by miR-21 in the pathogenesis of juvenile idiopathic arthritis Pubmed: 31641939 |
Biotechnology and Applied Biochemistry | Epigallocatechin 3‐gallate attenuates arthritis by regulating Nrf‐2, HO‐1 and cytokine levels in an experimental arthritis model Pubmed: 31746064 |
Microbial Pathogenesis | Tangeretin attenuates lipopolysaccharide-induced acute lung injury through Notch signaling pathway via suppressing Th17 cell response in mice Pubmed: 31676364 |
International Journal of Rheumatic Diseases | Effects of thalidomide on Th17, Treg cells and TGF‐β1/Smad3 pathway in a mouse model of systemic sclerosis Pubmed: 31840939 |
Cytokine | Levels of pro-and anti-inflammatory cytokines in cystic fibrosis patients with or without gingivitis Pubmed: 31927460 |
international urology and nephrology | The therapeutic effect of dendritic cells expressing indoleamine 2, 3-dioxygenase (IDO) on an IgA nephropathy mouse model Pubmed: 31894556 |
JOURNAL OF PERIODONTAL RESEARCH | Salivary Del‐1, IL‐17, and LFA‐1 levels in periodontal health and disease Pubmed: 32153040 |
journal of molecular endocrinology | LncRNA Profile in Hashimoto's Thyroiditis and Potential Function of NONHSAT079547. 2 Pubmed: 32229697 |
Toxicol Appl Pharmacol | Daphnetin ameliorates glucocorticoid-induced osteoporosis via activation of Wnt/GSK-3β/β-catenin signaling Pubmed: 33171191 |
CARDIOVASCULAR DRUGS AND THERAPY | Rhodiola crenulata reduces ventricular arrhythmia through mitigating the activation of IL-17 and inhibiting the MAPK signaling pathway Pubmed: 32946036 |
Biomed Research International | Effects of 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin on T Cell Differentiation in Primary Biliary Cholangitis Pubmed: 32908870 |
J Neurosurg | Covering the proximal nerve stump with chondroitin sulfate proteoglycans prevents traumatic painful neuroma formation by blocking axon regeneration after … Pubmed: 32470939 |
JOURNAL OF MICROBIOLOGY | Melatonin attenuates microbiota dysbiosis of jejunum in short-term sleep deprived mice Pubmed: 32424577 |
bioscience reports | Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in rat Pubmed: 32395767 |
Journal of Biochemical and Molecular Toxicology | Vitamin D3 and calcium cosupplementation alleviates cadmium hepatotoxicity in the rat: Enhanced antioxidative and anti‐inflammatory actions by remodeling cellular … |
Revista da Associacao Medica Brasileira | A comparison of IL-17 and IL-34 concentrations in the cerebrospinal fluid of patients with acute inflammatory demyelinating neuropathy and chronic … |
biosciences biotechnology research asia | Dietary Malted Barley Grain Improved Structure and Function of Spinal Cord of Mother Rats fed on a high cholesterol diet. |
ACS Biomaterials Science & Engineering | Tissue-Engineered Decellularized Allografts for Anterior Cruciate Ligament Reconstruction |
Effects of minocycline on neuroinflammation, gut microbiome and hippocampal neurogenesis in rats with Gulf War Illness | |
Minocycline Alleviate A Rat Model Of Gulf War Illness Via Altering Gut Microbiome, Attenuating Neuroinflammation And Enhancing Hippocampal Neurogenesis | |
中国应用生理学杂志 | 丹参对非酒精性脂肪肝Th17细胞及相关细胞因子的影响* |
Rev Assoc Med Bras | A comparison of IL-17 and IL-34 concentrations in the cerebrospinal fluid of patients with acute inflammatory demyelinating neuropathy and chronic?¡ 33295414 |
BEHAVIOURAL BRAIN RESEARCH | Minocycline alleviates Gulf War Illness rats via altering gut microbiome, attenuating neuroinflammation and enhancing hippocampal neurogenesis 34000339 |
Int J Clin Pract | Serum Galectin©\3 Levels in Patients with Psoriasis 34137138 |
cells | The Effect of the Clenbuterol¡ª¦Â2-Adrenergic Receptor Agonist on the Peripheral Blood Mononuclear Cells Proliferation, Phenotype, Functions, and Reactive Oxygen?¡ 33920705 |
Mediators of Inflammation | Sparstolonin B Exerts Therapeutic Effects on Collagen-Induced Arthritis by Inhibiting the NLRP3 Inflammasome and Reducing the Activity of ¦Á1, 3 ¡ |
CNS Neurosci Ther | Alterations in the gut microbiome with hemorrhagic transformation in experimental stroke 34591349 |
PLoS One | Evaluation of the involvement of Th17-cells in the pathogenesis of canine spinal cord injury 34591917 |
J Periodontol | Cytokine profile in serum and gingival crevicular fluid of children with inflammatory bowel disease: A case‐control study 34730850 |
Transl Psychiatry | Associations between expression of indoleamine 2, 3-dioxygenase enzyme and inflammatory cytokines in patients with first-episode drug-naive … 34802039 |
Viruses | Interleukin-10-Mediated Lymphopenia Caused by Acute Infection with Foot-and-Mouth Disease Virus in Mice 34960627 |
PLoS One | Th17 cell-mediated immune response in a subpopulation of dogs with idiopathic epilepsy Pubmed:35025939 |
Pathogens | Elevated Levels of IL-33, IL-17 and IL-25 Indicate the Progression from Chronicity to Hepatocellular Carcinoma in Hepatitis C Virus Patients Pubmed:35056005 |
Biomed Pharmacother | Jiangu granule ameliorated OVX rats bone loss by modulating gut microbiota-SCFAs-Treg/Th17 axis Pubmed:35453007 |
npj Biofilms and Microbiomes | The microbiota-gut-kidney axis mediates host osmoregulation in a small desert mammal Pubmed:35379849 |
Expert Opinion on Therapeutic Targets | Dapagliflozin mitigates ovalbumin-prompted airway inflammatory-oxidative successions and associated bronchospasm in a rat model of allergic asthma Pubmed:35549595 |
Catalog No. | Related products for research use of Sus scrofa; Porcine (Pig) Organism species | Applications (RESEARCH USE ONLY!) |
APA063Po61 | Active Interleukin 17 (IL17) | Cell culture; Activity Assays. |
RPA063Po01 | Recombinant Interleukin 17 (IL17) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA063Po01 | Polyclonal Antibody to Interleukin 17 (IL17) | WB; IHC; ICC; IP. |
MAA063Po21 | Monoclonal Antibody to Interleukin 17 (IL17) | WB; IHC; ICC; IP. |
SEA063Po | ELISA Kit for Interleukin 17 (IL17) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA063Po | Multiplex Assay Kit for Interleukin 17 (IL17) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |