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- Featured-Product
ELISA Kit for Interferon Gamma (IFNg)
IFN-G; IFG; IFI; INFr; IFN, Immune Interferon
- Product No.SEA049Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range15.6-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 6.1pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 326
For more details, please contact local distributors! US$ 465 US$ 2093 US$ 3953 US$ 32550
Specificity
This assay has high sensitivity and excellent specificity for detection of Interferon Gamma (IFNg).
No significant cross-reactivity or interference between Interferon Gamma (IFNg) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Interferon Gamma (IFNg) and the recovery rates were calculated by comparing the measured value to the expected amount of Interferon Gamma (IFNg) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 93-102 | 98 |
EDTA plasma(n=5) | 78-101 | 93 |
heparin plasma(n=5) | 99-105 | 102 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Gamma (IFNg) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Gamma (IFNg) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interferon Gamma (IFNg) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 91-98% | 96-103% | 81-99% | 94-102% |
EDTA plasma(n=5) | 78-96% | 83-94% | 88-103% | 79-97% |
heparin plasma(n=5) | 91-99% | 97-105% | 80-90% | 94-102% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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J Inflamm Res | Clenbuterol, a Selective β2-Adrenergic Receptor Agonist, Inhibits or Limits Post-Stroke Pneumonia, but Increases Infarct Volume in MCAO Mice Pubmed:35058704 |
Poult Sci | Salmonella phage CKT1 significantly relieves the body weight loss of chicks by normalizing the abnormal intestinal microbiome caused by hypervirulent … Pubmed:35063807 |
ACS Appl Mater Interfaces | Combinational Chemoimmunotherapy for Breast Cancer by Codelivery of Doxorubicin and PD-L1 siRNA Using a PAMAM-Incorporated Liposomal Nanoplatform Pubmed:35138103 |
Theranostics | Combating multidrug resistance and metastasis of breast cancer by endoplasmic reticulum stress and cell-nucleus penetration enhanced … Pubmed:35401832 |
Inflammopharmacology | Silymarin constrains diacetyl-prompted oxidative stress and neuroinflammation in rats: involvements of Dyn/GDNF and MAPK signaling pathway Pubmed:35366745 |
Journal of Nanobiotechnology | Biomimetic GBM-targeted drug delivery system boosting ferroptosis for immunotherapy of orthotopic drug-resistant GBM Pubmed:35351131 |
Infection and Drug Resistance | Sweet's Syndrome Accompanied by Coinfection with Multiple Pathogens and Disseminated Mycobacterium phlei Infection Presenting with Osteolytic … Pubmed:35592103 |
Sci Rep | Clinical findings and predictive factors for positive anti-interferon-γ autoantibodies in patients suffering from a non-tuberculosis mycobacteria or Talaromyces … Pubmed:35641599 |
Biomedicines | Deletion of Toxoplasma Rhoptry Protein 38 (PruΔrop38) as a Vaccine Candidate for Toxoplasmosis in a Murine Model Pubmed:35740356 |
Viruses | Synergistic Pathogenicity by Coinfection and Sequential Infection with NADC30-like PRRSV and PCV2 in Post-Weaned Pigs Pubmed:35215787 |
Antioxidants | Blue Light Alters the Composition of the Jejunal Microbiota and Promotes the Development of the Small Intestine by Reducing Oxidative Stress Pubmed:35204158 |
Oxidative Medicine and Cellular Longevity | The Role of Aeromonas-Goblet Cell Interactions in Melatonin-Mediated Improvements in Sleep Deprivation-Induced Colitis Pubmed:35355860 |
Journal of Integrative Medicine | Salvadora persica extract attenuates cyclophosphamide-induced hepatorenal damage by modulating oxidative stress, inflammation and apoptosis in rats Pubmed:35643766 |
researchsquare | MSC-EVs: A Promising Modulator for Immune Response in Systemic Lupus Erythematosus |
Mljekarstvo | Th1/Th2 cytokine polarization in milk according to different pathogens causing subclinical mastitis in cows |
Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
APA049Ra01 | Active Interferon Gamma (IFNg) | Cell culture; Activity Assays. |
EPA049Ra61 | Eukaryotic Interferon Gamma (IFNg) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA049Ra02 | Recombinant Interferon Gamma (IFNg) | Positive Control; Immunogen; SDS-PAGE; WB. |
APA049Ra61 | Active Interferon Gamma (IFNg) | Cell culture; Activity Assays. |
RPA049Ra01 | Recombinant Interferon Gamma (IFNg) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA049Ra01 | Polyclonal Antibody to Interferon Gamma (IFNg) | WB; IHC; ICC; IP. |
PAA049Ra02 | Polyclonal Antibody to Interferon Gamma (IFNg) | WB; IHC; ICC; IP. |
PAA049Ra06 | Polyclonal Antibody to Interferon Gamma (IFNg) | WB; IHC; ICC; IP. |
LAA049Ra81 | FITC-Linked Polyclonal Antibody to Interferon Gamma (IFNg) | WB; IHC; ICC; IF. |
LAA049Ra71 | Biotin-Linked Polyclonal Antibody to Interferon Gamma (IFNg) | WB; IHC; ICC. |
MAA049Ra22 | Monoclonal Antibody to Interferon Gamma (IFNg) | WB; IHC; ICC; IP. |
MAA049Ra23 | Monoclonal Antibody to Interferon Gamma (IFNg) | WB; IHC; ICC; IP. |
MAA049Ra21 | Monoclonal Antibody to Interferon Gamma (IFNg) | WB; IHC; ICC; IP. |
MEA049Ra | Mini Samples ELISA Kit for Interferon Gamma (IFNg) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SEA049Ra | ELISA Kit for Interferon Gamma (IFNg) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA049Ra | CLIA Kit for Interferon Gamma (IFNg) | Chemiluminescent immunoassay for Antigen Detection. |
LMA049Ra | Multiplex Assay Kit for Interferon Gamma (IFNg) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
KSA049Ra01 | ELISA Kit DIY Materials for Interferon Gamma (IFNg) | Main materials for "Do It (ELISA Kit) Yourself". |
CGA049Ra01 | cDNA of Interferon Gamma (IFNg) | PCR; Protein Expression; etc. |