CLIA Kit for Procollagen I C-Terminal Propeptide (PICP)

P1CP; C-Propeptide Of Type I Procollagen; Procollagen I Carboxy Terminal Propeptide

Specificity

This assay has high sensitivity and excellent specificity for detection of Procollagen I C-Terminal Propeptide (PICP).
No significant cross-reactivity or interference between Procollagen I C-Terminal Propeptide (PICP) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Procollagen I C-Terminal Propeptide (PICP) and the recovery rates were calculated by comparing the measured value to the expected amount of Procollagen I C-Terminal Propeptide (PICP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 79-103 85
EDTA plasma(n=5) 82-97 88
heparin plasma(n=5) 85-101 90

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Procollagen I C-Terminal Propeptide (PICP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Procollagen I C-Terminal Propeptide (PICP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Procollagen I C-Terminal Propeptide (PICP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 96-103% 84-99% 99-105% 78-94%
EDTA plasma(n=5) 88-95% 98-105% 90-98% 78-91%
heparin plasma(n=5) 97-104% 81-93% 81-97% 92-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

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Magazine Citations
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American Heart Journal Serum markers of deranged myocardial collagen turnover: their relation to malignant ventricular arrhythmias in cardioverter-defibrillator recipients with heart failure. PubMed: 23067911
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Scandinavian Journal of Rheumatology The association between insulin-like growth factor 1 (IGF-1), IGF-binding proteins (IGFBPs), and the carboxyterminal propeptide of type I procollagen (PICP) in pre- and postmenopausal women with rheumatoid arthritis. pubmed:27775453
Allergy and Asthma Proceedings Effect of montelukast on markers of airway remodeling in children withasthma. pubmed:27657514
Scandinavian Journal of Rheumatology The association between insulin-like growth factor 1 (IGF-1), IGF-binding proteins (IGFBPs), and the carboxyterminal propeptide of type I procollagen (PICP) in pre- and postmenopausal women with rheumatoid arthritis loi:irhe20
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Advanced Healthcare Materials Prognostic value of fibrosis-related markers in dilated cardiomyopathy: A link between osteopontin and cardiovascular events. pubmed:29120858
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Cardiovascular Journal of Africa Assessment of myocardial fibrosis by late gadolinium enhancement imaging and biomarkers of collagen metabolism in chronic rheumatic mitral regurgitation. Pubmed:29443354
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