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Recombinant Microphthalmia Associated Transcription Factor (MITF)

WS2A; MI; bHLHe32; Homolog Of Mouse Microphthalmia; Class E basic helix-loop-helix protein 32

  • Product No.RPH645Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationNucleus, Cytoplasm
  • Predicted Molecular Mass43.4kDa
  • Accurate Molecular Mass44kDa(Analysis of differences refer to the manual)
  • Residues & TagsAsn180~Gly290 with N-terminal His and GST Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 90%
  • Isoelectric Point11.0
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 148  US$ 370  US$ 740  US$ 2220  US$ 5550 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Toxicology in Vitro Melanogenesis and antioxidant defense system in normal human melanocytes cultured in the presence of chlorpromazine Pubmed:25449126
Naunyn Schmiedebergs Arch Pharmacol Effect of thioridazine on antioxidant status of HEMn-DP melanocytes PubMed: 26105001
Acta Poloniae Pharmaceutica ñ Drug Research FLUPHENAZINE AND PERPHENAZINE IMPACT ON MELANOGENESIS AND ANTIOXIDANT ENZYMES ACTIVITY IN NORMAL HUMAN MELANOCYTES 903.pdf
Die Pharmazie-An International Journal of Pharmaceutical Sciences Prochlorperazine interaction with melanin and melanocytes art00007
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPH645Mu01 Recombinant Microphthalmia Associated Transcription Factor (MITF) Positive Control; Immunogen; SDS-PAGE; WB.