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Recombinant Spondin 2 (SPON2)

DIL1; Mindin; M-spondin; Extracellular Matrix Protein; Differentially expressed in cancerous and non-cancerous lung cells 1

  • Product No.RPF396Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted, Extracellular matrix
  • Predicted Molecular Mass26.7kDa
  • Accurate Molecular Mass33kDa(Analysis of differences refer to the manual)
  • Residues & TagsVal119~Val330 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 95%
  • Isoelectric Point5.8
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 176  US$ 440  US$ 880  US$ 2640  US$ 6600 
    For more details, please contact local distributors!

SEQUENCE

Figure. Gene Sequencing (Extract)

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
The Journal of Urology Spondin-2, a secreted extracellular matrix protein, is a novel diagnostic biomarker for prostate cancer Pubmed: 23665271
Oxford Journals SP136EVALUATION OF SERUM SPONDIN 2 LEVELS IN THE DIFFERENT ETIOLOGIES OF GLOMERULAR DISEASES Content: 30
Nephrology (Carlton) Evaluation of Serum Spondin 2 Levels in the Different Stages of Type 2 Diabetic Nephropathy PubMed: 25973958
Oncogene The extracellular matrix protein mindin attenuates colon cancer progression by blocking angiogenesis via Egr-1-mediated regulation pubmed:28991232
Endocrinology Study of human T21 placenta suggests a potential role of mesenchymal spondin-2 in placental vascular development Pubmed: 30715257
Journal of Cellular and Molecular Medicine Mindin serves as a tumour suppressor gene during colon cancer progression through MAPK/ERK signalling pathway in mice Pubmed: 32614521
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPF396Mu01 Recombinant Spondin 2 (SPON2) Positive Control; Immunogen; SDS-PAGE; WB.
RPF396Mu02 Recombinant Spondin 2 (SPON2) Positive Control; Immunogen; SDS-PAGE; WB.
PAF396Mu01 Polyclonal Antibody to Spondin 2 (SPON2) WB; IHC; ICC; IP.
PAF396Mu02 Polyclonal Antibody to Spondin 2 (SPON2) WB; IHC; ICC; IP.
SEF396Mu ELISA Kit for Spondin 2 (SPON2) Enzyme-linked immunosorbent assay for Antigen Detection.
LMF396Mu Multiplex Assay Kit for Spondin 2 (SPON2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.